LOCUS       sequence-20160-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Schweitzer R, Chyung JH, Murtaugh LC, Brent AE, Rosen V, Olson EN, 
            Lassar A, Tabin CJ
  TITLE     Analysis of the tendon cell fate using Scleraxis, a specific marker 
            for tendons and ligaments.
  JOURNAL   Development. 2001 Oct . 128(19):3855-66.
  PUBMED    11585810
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2001 Oct . 128(19):3855-66."
COMMENT     Sequence Label: pBS cScx 3'UTR (CT#485)
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(575..593)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(627..649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    638..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgaggtcgac ggtatcgata agcttgatga tctgcacttt ctgcctcagc aaccagagga
       61 agctgagtaa agaccgcgac aggaagacgg cgatccggag ctaggcgctc ccggggaagg
      121 acggcacgga ggagcggaag gacggacgga cggacggaaa agccacacac accgaaccac
      181 ggacacactc ccgtcttcac cccgctgcca gggacaggac cctgccccta tggggccgtg
      241 aggctgggct gtggggacgc cccccgctgg ggactgagcc aagctgggga cgctgccgcc
      301 gggaccgacc gctgccgtgc tgttcccctc cagctttgag ccacgccatc ccctgaactg
      361 aggcccgggg tgctgcattt gttgagttta atcaatacat gctggtgttt catgtcattg
      421 atacgataat ataaagcctg agcagctcgt ataattaaaa atgagtacgg ttcaaaaaaa
      481 aaaaaaaaaa aaaagcggcc gcgaattcct gcagcccggg ggatccacta gttctagagc
      541 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta atttcnagct
      601 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      661 acaacatacg agccgg
//
