LOCUS       sequence-201600-        6749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-B150101 cloned as a His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  TITLE     HLA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMP71-HLA-B15-His
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1036..1055
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1036..1054
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1086..1110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2217..2234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2260..2284)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2529..2932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3438..4028
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4053..4069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4053..4069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4066..4088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4077..4093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4101..4107,4108..4125,4126..4131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4146..4167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4284..4301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4455..5043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5214..6005,6006..6074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5837..5856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6075..6179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6247..6265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6303..6325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6425..6444
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6638..6660
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6652..6669
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6653..6669
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
       61 ctgccccggc tcagggccaa gaacagttgg aacagcagaa tatgggccaa acaggatatc
      121 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
      181 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
      241 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
      301 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
      361 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
      421 aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg
      481 gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc
      541 cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt
      601 gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttggctaa ctagatctgt
      661 atctggcggt cccgcggaag aactgacgag ttcgtattcc cggccgcagc ccctgggaga
      721 cgtcccagcg gcctcggggg cccgttttgt ggcccattct gtatcagtta acctacccga
      781 gtcggacttt ttggagctcc gccactgtcc gaggggtacg tggctttgtt gggggacgag
      841 agacagagac acttcccgcc cccgtctgaa tttttgcttt cggttttacg ccgaaaccgc
      901 gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
      961 tatttgtctg aaaattagct cgacaaagtt aagtaatagt ccctctctcc aagctcactt
     1021 acaggcggcc tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt
     1081 gatcaacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgctggtca
     1141 tggcgccccg aaccgtcctc ctgctgctct cgggagccct ggccctgacc gagacctggg
     1201 ccggctccca ctccatgagg tatttctaca ccgccatgtc ccggcccggc cgcggggagc
     1261 cccgcttcat cgcagtgggc tacgtggacg acacccagtt cgtgaggttc gacagcgacg
     1321 ccgcgagtcc gaggatggcg ccccgggcgc catggataga gcaggagggg ccggagtatt
     1381 gggaccggga gacacagatc tccaagacca acacacagac ttaccgagag agcctgcgga
     1441 acctgcgcgg ctactacaac cagagcgagg ccgggtctca caccctccag aggatgtacg
     1501 gctgcgacgt ggggccggac gggcgcctcc tccgcgggca tgaccagtcc gcctacgacg
     1561 gcaaggatta catcgccctg aacgaggacc tgagctcctg gaccgcggcg gacacggcgg
     1621 ctcagatcac ccagcgcaag tgggaggcgg cccgtgaggc ggagcagtgg agagcctacc
     1681 tggagggcct gtgcgtggag tggctccgca gatacctgga gaacgggaag gagacgctgc
     1741 agcgcgcgga ccccccaaag acacatgtga cccaccaccc catctctgac catgaggcca
     1801 ccctgaggtg ctgggccctg ggcttctacc ctgcggagat cacactgacc tggcagcggg
     1861 atggcgagga ccaaactcag gacaccgagc ttgtggagac cagaccagca ggagatagaa
     1921 ccttccagaa gtgggcagct gtggtggtgc cttctggaga agagcagaga tacacatgcc
     1981 atgtacagca tgaggggctg ccgaagcccc tcaccctgag atgggagcca tcttcccagt
     2041 ccaccatccc catcgtgggc attgttgctg gcctggctgt cctagcagtt gtggtcatcg
     2101 gagctgtggt cgctactgtg atgtgtagga ggaagagctc aggtggaaaa ggagggagct
     2161 actctcaggc tgcgtccagc gacagtgccc agggctctga tgtgtctctc acagctcatc
     2221 accatcacca tcattgactc gagaagggtg ggcgcgccga cccagctttc ttgtacaaag
     2281 tggttgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct caattcgagc
     2341 atcttaccgc catttattcc catatttgtt ctgtttttct tgatttgggt atacatttaa
     2401 atgttaataa aacaaaatgg tggggcaatc atttacattt tatgggatat gtaattacta
     2461 gttcaggtgt attgccacaa gacaaacatg ttaagaaact ttcccgttat ttacgctctg
     2521 ttcctgttaa tcaacctctg gattacaaaa tttgtgaaag attgactgat attcttaact
     2581 atgttgctcc ttttacgctg tgtggatatg ctgctttaat gcctctgtat catgctattg
     2641 cttcccgtac ggctttcgtt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2701 aggagttgtg gcccgttgtc cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa
     2761 cccccactgg ctggggcatt gccaccacct gtcaactcct ttctgggact ttcgctttcc
     2821 ccctcccgat cgccacggca gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2881 ctaggttgct gggcactgat aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     2941 ggctgctcgc ctgtgttgcc aactggatcc tgcgcgggac gtccttctgc tacgtccctt
     3001 cggctctcaa tccagcggac ctcccttccc gaggccttct gccggttctg cggcctctcc
     3061 cgcgtcttcg ctttcggcct ccgacgagtc ggatctccct ttgggccgcc tccccgcctg
     3121 tttcgcctcg gcgtccggtc cgtgttgctt ggtcgtcacc tgtgcagaat tgcgaaccat
     3181 ggattccacc gtgaactttg tctcctggca tgcaaatcgt caacttggca tgccaagaat
     3241 aattcggatc caagcttagg cctgctcgct ttcttgctgt cccatttcta ttaaaggttc
     3301 ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg
     3361 gattctgcct agcgctaagc ttaacacgag ccatagatag aataaaagat tttatttagt
     3421 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     3481 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     3541 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     3601 ccccggctca gggccaagaa cagttggaac agcagaatat gggccaaaca ggatatctgt
     3661 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     3721 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     3781 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     3841 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     3901 agactgcgtc gcccgggtac ccgtgttctc aataaaccct cttgcagttg catccgactc
     3961 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactgccca cctcgggggt
     4021 ctttcattct cgagcagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcgccctgga
     4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6181 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     6241 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6301 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6361 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6481 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     6541 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6601 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6661 acggccagtg aattagtact ctagcttaag taacgccatt ttgcaaggca tggaaaatac
     6721 ataactgaga atagagaagt tcagatcaa
//
