LOCUS       sequence-201601-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-B150101 cloned as a His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  TITLE     HLA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR-HLA-B15-His
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      135..162
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(321..338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(492..1080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(572..591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1227..2036)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1950..1969
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2028..2047
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2149..2165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2149..2165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2206..2305
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2331..2348)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(3455..3554)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3570..3587)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3570..3586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3579..3601)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      join(3650..3693,1..43)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
       61 gaacgttgtg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
      121 gcatcaaact aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
      181 tcctggctag cggtacgcgt attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      241 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      301 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      481 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      901 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1021 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1081 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc gtaactcacg
     1141 ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat gctctgccag
     1201 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     1261 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     1321 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     1381 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     1441 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     1501 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     1561 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     1621 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     1681 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt ttttccgggg
     1741 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     1801 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     1861 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga
     1921 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     1981 gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg gctcataaca
     2041 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     2101 tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct
     2161 atgaccatgt aatacgactc actatagggg atatcagctg gatggcaaat aatgatttta
     2221 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg
     2281 ccaactttgt acaagaaagc tgggtcggcg cgcccaccct tctcgagtca atgatggtga
     2341 tggtgatgag ctgtgagaga cacatcagag ccctgggcac tgtcgctgga cgcagcctga
     2401 gagtagctcc ctccttttcc acctgagctc ttcctcctac acatcacagt agcgaccaca
     2461 gctccgatga ccacaactgc taggacagcc aggccagcaa caatgcccac gatggggatg
     2521 gtggactggg aagatggctc ccatctcagg gtgaggggct tcggcagccc ctcatgctgt
     2581 acatggcatg tgtatctctg ctcttctcca gaaggcacca ccacagctgc ccacttctgg
     2641 aaggttctat ctcctgctgg tctggtctcc acaagctcgg tgtcctgagt ttggtcctcg
     2701 ccatcccgct gccaggtcag tgtgatctcc gcagggtaga agcccagggc ccagcacctc
     2761 agggtggcct catggtcaga gatggggtgg tgggtcacat gtgtctttgg ggggtccgcg
     2821 cgctgcagcg tctccttccc gttctccagg tatctgcgga gccactccac gcacaggccc
     2881 tccaggtagg ctctccactg ctccgcctca cgggccgcct cccacttgcg ctgggtgatc
     2941 tgagccgccg tgtccgccgc ggtccaggag ctcaggtcct cgttcagggc gatgtaatcc
     3001 ttgccgtcgt aggcggactg gtcatgcccg cggaggaggc gcccgtccgg ccccacgtcg
     3061 cagccgtaca tcctctggag ggtgtgagac ccggcctcgc tctggttgta gtagccgcgc
     3121 aggttccgca ggctctctcg gtaagtctgt gtgttggtct tggagatctg tgtctcccgg
     3181 tcccaatact ccggcccctc ctgctctatc catggcgccc ggggcgccat cctcggactc
     3241 gcggcgtcgc tgtcgaacct cacgaactgg gtgtcgtcca cgtagcccac tgcgatgaag
     3301 cggggctccc cgcggccggg ccgggacatg gcggtgtaga aatacctcat ggagtgggag
     3361 ccggcccagg tctcggtcag ggccagggct cccgagagca gcaggaggac ggttcggggc
     3421 gccatgacca gcatggtgaa gggggcggcc gcggagcctg cttttttgta caaagttggc
     3481 attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     3541 aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg
     3601 gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg
     3661 aaaggctcag tcggaagact gggcctttcg ttt
//
