LOCUS       sequence-201618-       12646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of EGFP-SAP97beta fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FUG+p97W (C2)
FEATURES             Location/Qualifiers
     source          1..12646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3670..3689
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3879..3881,3882..3884,3885..4595)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3924..3945)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4185..4204)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4532..4553
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7338..7926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7391..7411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7809..7820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7929..7945)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7930..7946)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8451..8631
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8657..8674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8663..8887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8933..9361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9020..9039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9230..9251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9370..9390)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9375..9704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9555..9690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9617..9636
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9752..9799
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9818..10192
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10322..10443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10359..10378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10413..10432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10492..10508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10492..10508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10505..10527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10516..10532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10540..10546,10547..10564,10565..10570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10585..10606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10723..10740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10894..11482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10974..10993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11653..12444,12445..12513))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12276..12295
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12514..12618)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag aggatccacc ggtccaccat ggtgagcaag ggcgaggagc
     3901 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3961 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     4021 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     4081 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4141 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4201 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4261 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4321 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga
     4381 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     4441 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4501 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4561 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
     4621 cttcgaattc tatgccggtc cggaagcaag atacccagag agcattgcat ctgttggaag
     4681 aatatcggtc gaaactaagc caaaccgaag acagacagct caggagttcc atagagcggg
     4741 ttattagcat atttcagagc aacctctttc aggctttaat agacattcaa gaattttatg
     4801 aagtgacctt acttgataat ccaaaatgtg tggatcattc aaagcagtgt gaaccggtcc
     4861 aacccgggaa tccttgggag agtggcagcc tttcaagtgc tgctgtgact tcagagagcc
     4921 tgcccggcgg ccttagccct ccagtagaga aataccggta tcaggacgaa gaggtgctgc
     4981 cttcagagcg gatttccccg caagtcccaa atgaggtgct gggtccggag ctggttcacg
     5041 tctcggagaa gagcctgtca gagattgaga atgtccacgg gtttgtctct cactctcata
     5101 tctcacccat aaaggcaaat cctcctcctg tgctggtcaa cacagacagc ttggagacac
     5161 caacttatgt taatggtact gatgcagatt atgaatatga ggaaatcaca cttgaaaggg
     5221 gaaattcagg ccttggtttc agcattgcag gaggtacaga caacccacac attggagatg
     5281 actcaagtat tttcatcacc aaaattatca cgggaggagc agctgcccag gatggaagat
     5341 tgcgggtaaa tgactgtata ctgagagtaa atgaagcaga tgttcgtgat gtaacccaca
     5401 gcaaagcagt tgaagcattg aaagaagctg gatcgatagt gcgattatat gtgaaaaggc
     5461 ggaagccagc ctcagaaaaa atcatggaaa taaaacttat taaaggccct aaaggtcttg
     5521 ggttcagcat tgctggaggt gttggaaacc agcacattcc tggggataac agcatctatg
     5581 taaccaaaat aattgaagga ggtgcagcac acaaggacgg caaacttcag attggagaca
     5641 agcttctagc agtgaacagt gtctgtttag aagaagttac tcatgaagaa gcagtgactg
     5701 ccttaaagaa tacatctgat tttgtttatt tgaaagtggc aaaaccaaca agtatgtata
     5761 taaatgatgg ctatgcacca cccgacatca ctaattcttc ttctcagtct gttgacaacc
     5821 atgttagccc gtcttcatac ttgggccaga ctccagcgtc accagccaga tactcaccca
     5881 tttctaaagc agtgcttgga gatgatgaga tcactaggga acctagaaaa gttgttcttc
     5941 atcgtggctc aacgggactt ggtttcaaca ttgtgggagg cgaagatgga gaagggattt
     6001 ttatctcttt catccttgct gggggacctg ctgacctaag cggagagctc agaaaaggag
     6061 atcgcattat atcggtaaac agtgttgacc tcagagctgc aagtcatgaa caagcagctg
     6121 ccgcattaaa aaacgcaggc caagctgtca ctattgtcgc acagtatcgg cctgaagagt
     6181 acagtcgttt tgaagctaaa attcacgact tgcgggagca gatgatgaac agcagtgtca
     6241 gctcagggtc agggtctctc cgaaccagcc aaaagcggtc cctctatgtc agagcccttt
     6301 ttgattacga caagactaaa gacagtggcc ttcccagtca agggttgaac ttcaaattcg
     6361 gagatattct ccatgttatc aatgcttctg atgacgagtg gtggcaggcc agacaggtga
     6421 ccccagatgg tgagagtgac gaagttggag taattcctag taaacgcaga gttgagaaga
     6481 aagaacgagc ccgattaaaa acagtcaaat tcaattctaa aacaagagga gataaagggt
     6541 cattcaatga caagcgtaaa aagaacctct tttcccgaaa atttcccttc tacaagaaca
     6601 aggaccagag tgagcaggaa acaagtgatg ctgaccagca cgtaacttct aatgccagcg
     6661 atagcgaaag tagttaccgt ggtcaagaag aatatgtttt atcgtatgag ccggtgaatc
     6721 agcaagaagt taattatacc cgaccagtca tcatattagg acctatgaaa gacagagtaa
     6781 atgatgactt aatctcagaa tttcctgaca aatttggatc ctgtgtccct catacaacta
     6841 gaccaaagcg tgactatgag gtggatggac gagattacca ttttgtgact tcaagagaac
     6901 agatggagaa agatatccag gagcataaat tcattgaagc tggccagtat aacaaccatc
     6961 tatacggaac gagtgtccaa tctgtgcgag cagtagcaga gaagggcaag cattgtatcc
     7021 ttgatgtgtc tggaaatgcc atcaagaggt tgcagattgc acagctctat ccaatatcta
     7081 tttttattaa acccaaatcc atggaaaata tcatggaaat gaataaacgc ctaacagacg
     7141 aacaggccag aaaaacgttt gagagagcag tgagactgga gcaggagttc actgagcatt
     7201 tcacagctat tgtccaggga gacacgctgg aggacattta caatcaagtg aagcagatca
     7261 tagaagagca gtctggtcct tacatctggg tcccagcaaa agaaaaatta tgagaattcg
     7321 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7381 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7441 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7501 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7561 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7621 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7681 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7741 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7801 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7861 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7921 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     7981 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     8041 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8101 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8161 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     8221 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     8281 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     8341 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     8401 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     8461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     8641 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8881 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     8941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9361 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9421 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     9661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     9721 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     9781 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     9841 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     9901 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     9961 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
    10021 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
    10081 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
    10141 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
    10201 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10261 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10321 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10381 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10441 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    10501 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10561 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10621 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10681 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10741 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10801 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10861 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10921 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10981 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    11041 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    11101 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11161 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11221 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11281 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    11341 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11401 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11461 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11521 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11581 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11641 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11701 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11761 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11821 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11881 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11941 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    12001 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    12061 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    12121 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12181 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12241 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    12301 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    12361 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12421 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12481 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12541 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12601 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
