LOCUS       sequence-201620-        8121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDL17 is based on conditionally replicating backbone derived from 
            pQE-30 and expresses gam, bet and exo genes under control of PrhaB 
            promoter for efficient dsDNA integration into E. coli chromosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
  TITLE     Development of new versatile plasmid-based systems for 
            lambdaRed-mediated Escherichia coli genome engineering.
  JOURNAL   J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001.
  PUBMED    29885886
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001."
COMMENT     Sequence Label: pDL17
FEATURES             Location/Qualifiers
     source          1..8121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..154
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             188..604
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             610..1395
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             1392..2072
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      2073..2317
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     terminator      2478..2564
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2656..2683
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        complement(join(2718..2724,2725..2742,2743..2748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2763..2784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2797..3879)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3841..3860
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3880..3957)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(4004..4018,4019..4024,4025..4041,4042..4048)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    4024..4040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4056..4072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4091..4102
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4121..4138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4210..4227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4381..4969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4461..4480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5140..5931,5932..6000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5763..5782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6001..6105)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6131..6225
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6253..7101)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(7175..8011)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca aattgtgaac
       61 atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag
      121 gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcggatccc gtcgacgctt
      181 ataaaaaatg gatattaata ctgaaactga gatcaagcaa aagcattcac taaccccctt
      241 tcctgttttc ctaatcagcc cggcatttcg cgggcgatat tttcacagct atttcaggag
      301 ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca
      361 ctaccagcag ctcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
      421 cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag
      481 caaaaaatcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
      541 acacatccgg tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt
      601 ataaaacgaa tgagtactgc actcgcaacg ctggctggga agctggctga acgtgtcggc
      661 atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt
      721 gatgccagcg atgcgcagtt catcgcatta ctgatcgttg ccaaccagta cggccttaat
      781 ccgtggacga aagaaattta cgcctttcct gataagcaga atggcatcgt tccggtggtg
      841 ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt
      901 gagcaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc
      961 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga
     1021 gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt
     1081 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     1141 attgtcgaaa atactgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt
     1201 aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat
     1261 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa
     1321 ctgacacagg ccgaagcagt aaaagctctt ggattcctga aacagaaagc cgcagagcag
     1381 aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgat gtgagagctg
     1441 tcgaacaggg ggaagatgcg tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     1501 ttcacaacgt gatagcaaaa ccccgctccg gaaagaagtg gcctgacatg aaaatgtcct
     1561 acttccacac cctgcttgct gaggtttgca ccggtgtggc tccggaagtt aacgctaaag
     1621 cactggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg
     1681 gcgtgaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgt accgcctgct
     1741 ctcccgatgg tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc
     1801 gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaagtcagct tacatggccc
     1861 aggtgcagta cagcatgtgg gtgacgcgaa aaaatgcctg gtactttgcc aactatgacc
     1921 cgcgtatgaa gcgtgaaggc ctgcattatg tcgtgattga gcgggatgaa aagtacatgg
     1981 cgagttttga cgagatcgtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa
     2041 ttggttttgt atttggggag caatggcgat gacgcatcct cacgataata tccgggtagg
     2101 cgcaatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt
     2161 tatccgaaat ccactgaaag cacagcggct ggctgaggag ataaataata aacgaggggc
     2221 tgtatgcaca aagcatcttc tgttgagtta agaacgagta tcgagatggc acatagcctt
     2281 gctcaaattg gaatcaggtt tgtgccaata ccagtagaaa cagacgaaga attctaggct
     2341 ggagaatctc gatccggact ccctgacgcc acgccaggcg ctggagtgga tctatcggct
     2401 gaaaagtctg gtgtaaggat cgtggcagac aagcccgtca gggcgcgtcc atgcgagagt
     2461 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2521 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     2581 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     2641 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     2701 ttcctgccat ggccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2881 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     2941 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     3001 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     3061 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     3121 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     3181 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     3241 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     3301 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     3361 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     3421 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     3481 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     3541 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     3601 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     3661 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     3721 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     3781 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     3841 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     3901 ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga tggtgtcaac
     3961 gtaaatgcat gccgctaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
     4021 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
     4081 agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc accatcacgg
     4141 atctatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     4201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6121 agtgccacct gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     6181 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatgacgt ccgtcggcat
     6241 cggcatggcg aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc
     6301 ggtttcccgg gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga
     6361 aatatcactg attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac
     6421 ctgtcgcaga tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac
     6481 gcgctcactg cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct
     6541 agccgccagc cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaacga
     6601 atcactggtg taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactcagc
     6661 catttcgtta gcaaacggca catgctgact actttcatgc tcaagctgac cgataacctg
     6721 ccgcgcctgc gccatcccca tgctacctaa gcgccagtgt ggttgccctg cgctggcgtt
     6781 aaatcccgga atcgccccct gccagtcaag attcagcttc agacgctccg ggcaataaat
     6841 aatattctgc aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat
     6901 gtaaaagaga tcgccacggg taatgcgata agggcgatcg ttgagtacat gcaggccatt
     6961 accgcgccag acaatcacca gctcacaaaa atcatgtgta tgttcagcaa agacatcttg
     7021 cggataacgg tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag
     7081 aagttttaac tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc
     7141 aatatggcgt acaaatacgt tgagaagatt cgcgttattg cagaaagcca tcccgtccct
     7201 ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac
     7261 tgtcgctgaa tccacagcga taggcgatgt cagtaacgct ggcctcgctg tggcgtagca
     7321 gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg ctgaggcgtc agtcccgttt
     7381 gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga aaattgatcc gccacggcat
     7441 cccaattcac ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg
     7501 atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca
     7561 taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca
     7621 tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgagacg
     7681 gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc
     7741 gatccggcga gcgatacagc acattggtca gacacagatt atcggtatgt tcatacagat
     7801 gccgatcatg atcgcgtacg aaacagaccg tgccaccggt gatggtatag ggctgcccat
     7861 taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat
     7921 gttcaggaaa atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg
     7981 gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg
     8041 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa
     8101 acgtcgattt ttcaagatac a
//
