LOCUS       sequence-201622-       10934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH 
            domains using SapI restriction enzyme. Full Fc-mediated immune 
            effector functions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-5
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(991..1009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1077..1181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1182..1250,1251..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1400..1419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2213..2801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2702..2721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3232..3377
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3420..4457
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4455..4474)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4499..4547
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5201..5580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5581..5784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5735..5755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5781..5805
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5826..5835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6910..7044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6934..6953)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6988..7007
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7187..7209)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7769..9558
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct ccccctcctg
       61 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
      121 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
      181 ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
      241 ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt
      301 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
      361 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
      421 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
      481 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
      541 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
      601 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
      661 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
      721 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
      781 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
      841 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
      901 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
      961 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1021 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1081 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     1441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     1741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2341 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2581 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2821 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     2881 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     2941 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     3001 cggccccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     3061 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     3121 agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     3181 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     3241 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     3301 ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     3361 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata
     3421 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3481 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3541 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     3601 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     3661 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     3721 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     3781 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     3841 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     3901 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     3961 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     4021 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     4081 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     4141 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     4201 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4261 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4321 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4381 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga
     4441 tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4501 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     4561 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4621 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     4681 agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg
     4741 tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt
     4801 caggtccacg actggactga gcagacagac ccatggtttt tggatggcct gggcatggac
     4861 cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc
     4921 caaaaaccac cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac
     4981 agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag
     5041 gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     5101 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     5161 tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt
     5221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5461 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5521 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5581 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     5641 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5701 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     5761 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag aagctgggta
     5821 ccgccgccac catggactgg acctggaggg tgttctgcct gctggccgtg gcccccggcg
     5881 cccacagcga agagctacag agtgagctct tcgccagcac caagggtcca agtgtattcc
     5941 cactcgctcc aagctctaaa tccacgagcg gtggtacggc agccctcgga tgcctcgtca
     6001 aagattattt tccagaacct gttacagtat cttggaatag cggcgcgttg accagtggcg
     6061 tccatacatt tcccgcggta ttgcaatcat ctggcctcta tagtctttca tcagtggtca
     6121 ccgtcccgag ttctagcctg ggtacacaga cttacatttg caatgtaaac cataagccaa
     6181 gcaatactaa agttgacaag aaggtcgagc ccaaaagctg tgacaagact catacctgcc
     6241 cgccctgccc agcccccgaa cttcttggtg gaccgtccgt cttcctcttt cctcctaagc
     6301 cgaaggacac gctgatgatc tcaaggactc ccgaagttac atgtgtggtc gtagatgtct
     6361 cacacgagga ccccgaggtt aaattcaatt ggtacgtcga cggagtagaa gttcataacg
     6421 ccaaaactaa gccgagggag gagcaataca attcaacgta tcgggtcgta agcgttctta
     6481 ccgtcctcca tcaggattgg ttgaacggta aagaatacaa gtgcaaagtt agcaataaag
     6541 ccctccccgc cccgatagaa aagactataa gtaaggcgaa aggacaacca agagaacccc
     6601 aagtttacac tttgcccccc tcacgggatg aacttacaaa aaatcaagtc tcccttacat
     6661 gcctggtgaa gggtttctac ccatcagata tcgcagtgga atgggaatct aacggacaac
     6721 cagaaaataa ttacaagacg acgccgccag tcctcgactc tgatgggtcc ttttttttgt
     6781 actcaaaact tactgtggat aaaagtaggt ggcaacaagg caatgtgttc tcctgctcag
     6841 tgatgcatga ggcccttcac aatcattata cccagaaatc attgagcctg agccccggca
     6901 aatgataagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6961 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7021 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7081 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     7141 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7201 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7261 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     7321 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     7381 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     7441 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     7501 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     7561 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     7621 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     7681 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     7741 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     7801 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     7861 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     7921 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     7981 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     8041 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     8101 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     8161 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     8221 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     8281 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     8341 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     8401 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     8461 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     8521 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     8581 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     8641 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     8701 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     8761 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     8821 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     8881 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     8941 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9001 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     9061 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     9121 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     9181 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9241 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9301 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     9361 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     9421 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
     9481 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
     9541 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
     9601 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
     9661 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
     9721 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
     9781 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
     9841 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
     9901 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
     9961 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
    10021 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
    10081 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
    10141 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
    10201 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
    10261 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
    10321 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
    10381 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
    10441 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
    10501 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
    10561 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
    10621 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
    10681 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
    10741 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
    10801 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
    10861 ctgcccctcc tcctcctgcc cctcctgccc ctcctgctcc tccccctcct ccccctcctg
    10921 ctcctgcccc tcct
//
