LOCUS       sequence-201638-       11186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of 
            antibody VH domains using SapI restriction enzyme. Abolished 
            Fc-mediated immune effector functions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-6
FEATURES             Location/Qualifiers
     source          1..11186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1300..1318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1386..1490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1491..1559,1560..2351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1709..1728)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2522..3110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3011..3030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3541..3686
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3729..4766
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4764..4783)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4808..4856
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5510..5889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5890..6093
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6044..6064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6090..6114
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6135..6144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7219..7353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7243..7262)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7297..7316
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7496..7518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8078..9867
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
       61 cccctcctgc ccctcctgct cctgcccctc ctccccctcc tgctcctccc cctcctcccc
      121 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
      181 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
      241 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
      301 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
      361 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
      421 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
      481 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
      541 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
      601 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
      661 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
      721 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
      781 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
      841 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
      901 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
      961 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
     1021 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
     1081 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
     1141 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
     1201 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
     1261 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     1321 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1381 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1501 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1561 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1621 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1681 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     1741 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1801 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1861 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1921 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1981 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     2041 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2101 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2161 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2221 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2281 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2341 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2401 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2641 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2881 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3121 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
     3181 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     3241 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     3301 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     3361 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     3421 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
     3481 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
     3541 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
     3601 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     3661 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
     3721 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     3781 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     3841 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     3901 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     3961 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     4021 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     4081 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     4141 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     4201 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     4261 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     4321 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     4381 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     4441 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     4501 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     4561 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4621 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4681 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     4741 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4801 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     4861 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4921 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4981 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
     5041 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     5101 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     5161 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     5221 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
     5281 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     5341 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     5401 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     5461 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     5521 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5581 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5641 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5701 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5761 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5821 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5881 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     5941 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     6001 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     6061 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
     6121 agctgggtac cgccgccacc atggactgga cctggagggt gttctgcctg ctggccgtgg
     6181 cccccggcgc ccacagcgaa gagctacaga gtgagctctt cgccagcacc aaaggtccat
     6241 cagtattccc actcgcgcca agctccaaat ccacaagcgg ggggactgca gctctgggat
     6301 gcctcgtcaa agattatttc cccgagcccg tgactgtgag ttggaattct ggagcattga
     6361 ccagtggcgt tcacacgttt ccagcggttc tccagtcctc tggcttgtat tccctttcta
     6421 gtgtcgttac cgtcccatcc agcagtcttg gcacacaaac ttacatttgc aatgtaaatc
     6481 acaagccctc aaatacgaag gttgataaga aggtggaacc caaatcatgc gacaagacac
     6541 acacttgtcc cccttgccct gcacctgaag ctgaaggggc gccctctgtg tttctgtttc
     6601 caccgaagcc aaaggacacg cttatgattt cccgaacacc tgaggttact tgcgtcgttg
     6661 ttgacgtctc ccatgaagat ccagaagtga aattcaattg gtatgttgac ggcgttgagg
     6721 tccataatgc gaagactaag ccccgggagg agcagtataa cagcacctac agagttgtat
     6781 ccgtgcttac ggtacttcat caagattggt tgaacgggaa agagtataaa tgtgccgtct
     6841 ccaataaggc tctgcctgcg tctattgaaa agaccatttc aaaggccaaa ggtcaacccc
     6901 gggagcccca agtatataca ttgcccccct ctcgagacga gttgactaag aatcaagtca
     6961 gcttgacatg cttggtcaaa ggtttttacc cttcagatat agcggtagag tgggagtcca
     7021 acggccagcc tgaaaacaac tacaagacta ccccccctgt cctggatagc gatggatctt
     7081 tctttctgta tagtaaactg accgttgata agagtagatg gcagcaaggc aatgtcttct
     7141 cttgttctgt gatgcacgag gctttgcata atcactatac gcagaaaagc ttgtctctta
     7201 gcccgggaaa atgataagga tccagacatg ataagataca ttgatgagtt tggacaaacc
     7261 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     7321 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     7381 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     7441 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7501 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7561 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     7621 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     7681 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     7741 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     7801 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     7861 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     7921 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     7981 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     8041 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     8101 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     8161 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     8221 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     8281 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     8341 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     8401 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     8461 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     8521 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     8581 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     8641 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     8701 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     8761 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     8821 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     8881 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     8941 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     9001 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     9061 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     9121 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     9181 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     9241 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     9301 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     9361 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     9421 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     9481 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     9541 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9601 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     9661 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9721 atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat
     9781 acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt
     9841 gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg aatttaagga
     9901 ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc taatgttttc
     9961 caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt atgcccaatt
    10021 gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata caccaacagc
    10081 acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg cttttaatac
    10141 gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc tcatctccat
    10201 cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct tccaccatag
    10261 gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc accctgatat
    10321 tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc aaaacctcag
    10381 caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc cttagcgggc
    10441 caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat ggtgtaagac
    10501 gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag gtcttactac
    10561 ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac
    10621 tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg ttggaacctc
    10681 cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca tcaccctgac
    10741 cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc tctatcgctc
    10801 ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca aaataatcgg
    10861 cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc ggtggagacc
    10921 cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt ccacgacctc
    10981 tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta ccccggcggc
    11041 ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct ccactgcctc
    11101 ctcgaccccg gcctccacct cctgctcctg cccctcctgc tcctgcccct cctcctgctc
    11161 ctgcccctcc tgcccctcct gctcct
//
