LOCUS       sequence-201640-        3663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription activator (transactivation domain of viral VP16 
            protein) that is constitutively recruited lacO arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pEGFP-LacI-NLS-VP16
FEATURES             Location/Qualifiers
     source          1..3663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(452..472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        457..786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      637..772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      825..834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(831..833,834..836,837..1547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(876..897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1137..1156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1484..1505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1585..1604)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2664..2684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3067..3188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3104..3123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3158..3177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3195..3649)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3332..3353)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3543..3562
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
       61 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      421 cgtattaccg ccatgcatta gttattactg tggaatgtgt gtcagttagg gtgtggaaag
      481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      721 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      781 tgcaaaaagc tagcgctacc ggggccgctc tagcgctacc ggtcgccacc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     1561 ccatggtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     1621 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     1681 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     1741 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     1801 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     1861 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     1921 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     1981 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     2041 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     2101 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     2161 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     2221 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     2281 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     2341 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     2401 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     2461 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     2521 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     2581 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga agcagcctga
     2641 gggggcgtcg actgggtacc cctcctaaga agaagaggaa ggttgccccc accatggata
     2701 aaaagaagag aaaggccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg
     2761 gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg
     2821 gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc
     2881 tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccctcta gacgggggga
     2941 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     3001 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3061 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3181 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3241 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3301 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3361 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3421 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaagcact aaatcggaac
     3481 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3541 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3601 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3661 ttt
//
