LOCUS       sequence-201642-        3645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription activator (transactivation domain of viral VP16 
            protein) that is constitutively recruited lacO arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: ptagBFP-LacI-NLS-VP16
FEATURES             Location/Qualifiers
     source          1..3645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(452..472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        457..786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      637..772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             831..1529
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(1567..1586)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2646..2666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3049..3170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3086..3105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3140..3159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3177..3631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3314..3335)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3525..3544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
       61 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      421 cgtattaccg ccatgcatta gttattactg tggaatgtgt gtcagttagg gtgtggaaag
      481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      721 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      781 tgcaaaaagc tagcgctacc ggggccgctc tagcgctacc ggtcgccacc atgagcgagc
      841 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact
      901 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
      961 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct
     1021 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct
     1081 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     1141 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     1201 tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca
     1261 ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc
     1321 tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg
     1381 ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg
     1441 aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc
     1501 tccctagcaa actggggcac aagcttaatt ccggactcag atccatggtg aaaccagtaa
     1561 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     1621 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     1681 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     1741 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     1801 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     1861 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     1921 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     1981 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     2041 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     2101 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     2161 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     2221 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     2281 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     2341 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     2401 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     2461 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     2521 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     2581 cattaatgca gctggcacga caggtttccc gaagcagcct gagggggcgt cgactgggta
     2641 cccctcctaa gaagaagagg aaggttgccc ccaccatgga taaaaagaag agaaaggccc
     2701 ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg
     2761 cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg
     2821 gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg
     2881 agtttgagca gatgtttacc gatgcccctc tagacggggg gatccaccgg atctagataa
     2941 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3001 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3181 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3241 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3301 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3361 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3421 aagttttttg gggtcgaggt gccgtaagca ctaaatcgga accctaaagg gagcccccga
     3481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3601 gccgcgctta atgcgccgct acagggcgcg tcaggtggca ctttt
//
