LOCUS       sequence-20166-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Theodosiou NA, Tabin CJ
  TITLE     Wnt signaling during development of the gastrointestinal tract.
  JOURNAL   Dev Biol. 2003 Jul 15. 259(2):258-71.
  PUBMED    12871700
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2003 Jul 15. 259(2):258-71."
COMMENT     Sequence Label: pGEM cWnt10a (CT#588)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(439..457)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(440..457)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(475..491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(488..510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(523..529,530..547,548..553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    568..589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(706..723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actcactata gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg
       61 ggaattcgat tatgcggctg cacaataaca gggtggggcg gcaggtggtg atggacaaca
      121 tgaggaggaa gtgcaagtgc cacggcacct cggggagctg ccagctgaag acctgctggc
      181 aggtgacacc cgaattccgc ctggtggggt ccctcctcaa ggaccggttc tgtggggcca
      241 ctctcatccg gccccacaac cgcaatgcag ggcagctgga gccgggccac gcacggcatc
      301 gccgccgcgc cggcatcagc gagctggtct attttgagaa gtgccccgat ttttgtgaaa
      361 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
      421 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      841 ggccaggaac cgtaaaaagg
//
