LOCUS       sequence-201672-        6913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6913)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-TPX2-N-10
FEATURES             Location/Qualifiers
     source          1..6913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2809..2818
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2815..3516
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2948..2966)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3216..3235
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3410..3433
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3641..3762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3678..3697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3732..3751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3769..4224)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3906..3927)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4118..4137
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4251..4355
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4357..4714
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4357..4548
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4380..4400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4565..4700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4749..5543
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4749..5543
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4803..4822)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5413..5432
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5731..5750)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5775..5822
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6151..6739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6640..6659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccgcc
      541 accatgtcac aagttaaaag ctcttattcc tatgatgccc cctcggattt catcaatttt
      601 tcatccttgg atgatgaagg agatactcaa aacatagatt catggtttga ggagaaggcc
      661 aatttggaga ataagttact ggggaagaat ggaactggag ggctttttca gggcaaaact
      721 cctttgagaa aggctaatct tcagcaagct attgtcacac ctttgaaacc agttgacaac
      781 acttactaca aagaggcaga aaaagaaaat cttgtggaac aatccattcc gtcaaatgct
      841 tgttcttccc tggaagttga ggcagccata tcaagaaaaa ctccagccca gcctcagaga
      901 agatctctta ggctttctgc tcagaaggat ttggaacaga aagaaaagca tcatgtaaaa
      961 atgaaagcca agagatgtgc cactcctgta atcatcgatg aaattctacc ctctaagaaa
     1021 atgaaagttt ctaacaacaa aaagaagcca gaggaagaag gcagtgctca tcaagatact
     1081 gctgaaaaga atgcatcttc cccagagaaa gccaagggta gacatactgt gccttgtatg
     1141 ccacctgcaa agcagaagtt tctaaaaagt actgaggagc aagagctgga gaagagtatg
     1201 aaaatgcagc aagaggtggt ggagatgcgg aaaaagaatg aagaattcaa gaaacttgct
     1261 ctggctggaa tagggcaacc tgtgaagaaa tcagtgagcc aggtcaccaa atcagttgac
     1321 ttccacttcc gcacagatga gcgaatcaaa caacatccta agaaccagga ggaatataag
     1381 gaagtgaact ttacatctga actacgaaag catccttcat ctcctgcccg agtgactaag
     1441 ggatgtacca ttgttaagcc tttcaacctg tcccaaggaa agaaaagaac atttgatgaa
     1501 acagtttcta catatgtgcc ccttgcacag caagttgaag acttccataa acgaacccct
     1561 aacagatatc atttgaggag caagaaggat gatattaacc tgttaccctc caaatcttct
     1621 gtgaccaaga tttgcagaga cccacagact cctgtactgc aaaccaaaca ccgtgcacgg
     1681 gctgtgacct gcaaaagtac agcagagctg gaggctgagg agctcgagaa attgcaacaa
     1741 tacaaattca aagcacgtga acttgatccc agaatacttg aaggtgggcc catcttgccc
     1801 aagaaaccac ctgtgaaacc acccaccgag cctattggct ttgatttgga aattgagaaa
     1861 agaatccagg agcgagaatc aaagaagaaa acagaggatg aacactttga atttcattcc
     1921 agaccttgcc ctactaagat tttggaagat gttgtgggtg ttcctgaaaa gaaggtactt
     1981 ccaatcaccg tccccaagtc accagccttt gcattgaaga acagaattcg aatgcccacc
     2041 aaagaagatg aggaagagga cgaaccggta gtgataaaag ctcaacctgt gccacattat
     2101 ggggtgcctt ttaagcccca aatcccagag gcaagaactg tggaaatatg ccctttctcg
     2161 tttgattctc gagacaaaga acgtcagtta cagaaggaga agaaaataaa agaactgcag
     2221 aaaggggagg tgcccaagtt caaggcactt cccttgcctc attttgacac cattaacctg
     2281 ccagagaaga aggtaaagaa tgtgacccag attgaacctt tctgcttgga gactgacaga
     2341 agaggtgctc tgaaggcaca gacttggaag caccagctgg aagaagaact gagacagcag
     2401 aaagaagcag cttgtttcaa ggctcgtcca aacaccgtca tctctcagga gccctttgtt
     2461 cccaagaaag agaagaaatc agttgctgag ggcctttctg gttctctagt tcaggaacct
     2521 tttcagctgg ctactgagaa gagagccaaa gagcggcagg agctggagaa gagaatggct
     2581 gaggtagaag cccagaaagc ccagcagttg gaggaggcca gactacagga ggaagagcag
     2641 aaaaaagagg agctggccag gctacggaga gaactggtgc ataaggcaaa tccaatacgc
     2701 aagtaccagg gtctggagat aaagtcaagt gaccagcctc tgactgtgcc tgtatctccc
     2761 aaattctcca ctcgattcca ctgctcggga agcacggatc caccggtcgc caccatggtg
     2821 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     2881 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2941 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     3001 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     3061 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3121 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     3181 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     3241 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     3301 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     3361 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     3421 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     3481 cacctgttcc tgtacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     3541 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3601 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3721 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     3781 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3841 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3901 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3961 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4021 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4081 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4141 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4201 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4321 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     4381 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4441 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4561 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4621 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4681 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4741 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4801 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4861 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4921 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4981 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5041 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5101 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5161 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5221 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     5281 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5341 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5401 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5461 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5521 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5581 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5641 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5701 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5761 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5821 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5881 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5941 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     6001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6841 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     6901 atatggagtt ccg
//
