LOCUS       sequence-201689-        4193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine phospho-mimetic CEP215 at S608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4193)
  TITLE     Expresses murine phospho-mimetic CEP215 at S608
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: (PM-S608D)FLAG-Cep215-FL
FEATURES             Location/Qualifiers
     source          1..4193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        28..331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        332..535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     486..506
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     580..599
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        580..598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      612..621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             621..692
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
       61 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      121 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      181 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      241 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      301 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      361 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      421 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      481 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      541 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      601 gacccaagct tgccaccatg gattacaagg atgacgacga taaggattac aaggatgacg
      661 acgataagga ttacaaggat gacgacgata agggatctat ggactcgggg atggaagagg
      721 aaggcgccct gcctgggacc ctcagcggtt gcagtggcct tcaccctgtt ctgccaagtg
      781 acctggatgt catcagtgac acttctggct tgggaaatgg agtgctccca agcatgtcag
      841 aagaaaaggt ttctcccacc agagcccgga acatgaagga ctttgaaaac caaatcactg
      901 agctgaagaa ggagaacttc aatctaaagc tccggatcta cttcctggag gagagaatcc
      961 agcaggagtt tgctggcccc accgagcaca tctacaagac gaacattgaa ctcaaggtgg
     1021 aagtggaaag tctgaagcgc gagctccagg agaaagatca gttgctcgtc aaagcctcca
     1081 aagcggtgga gagcctagca gaacggggtg gttctgaagt ccagcgggtg aaagaagatg
     1141 caaggaagaa ggtgcagcag gtggaagacc tcctaacgaa aaggatacat cttctagaag
     1201 aagatgtgaa agccgcccag gcagaactgg agaaggcctt tgcggggaca gaaacagaga
     1261 aggctcttcg gctcagctta gaaagcaagc tctcggcgat gaagaagatg caggaggggg
     1321 acctggagat gactctggct ctggaagaga aggacagact gattgaggag ttgaagctgt
     1381 ctttgaagag caaagaagct ttaattcagt gccttaaaga ggaaaaatct cagatggcat
     1441 ctcctgacga gaacgtgtca tctggagaac tccgaggact ttctgccact ctgagggaag
     1501 aaaaagagag agacgctgag gaatggcaga aggagagaaa ccactttgaa gagaggatcc
     1561 aggcacttca ggaggacctg agagagaagg aaagagaaat tgctacagag aagaaaaata
     1621 acttaaagag ttataaagcc attcagggtc taaccatggc attaaaatca aaggaaagag
     1681 aggttgaaga acttgattcc aaaatcaaag aggtcactac ggactccacc aaaggcagag
     1741 aggatccctt aaaaacccag atccccagat tccagctaag agaggggtct gaggactgtg
     1801 aagctgcgct ggtagagaag gaagccctat tggccaagct acactcagaa aatgtcacaa
     1861 agagcactga gaaccatagg ttgctcagga atgttaaaaa ggtcacccag gagttgaatg
     1921 acttgaagaa ggagaagttg agactagagc aggccctgga ggaagcccac caagagggga
     1981 acagaggagc ccgaaccatt cacgacctta gaaatgaagt tgagaaacta cgcaaggaag
     2041 tgagtgagag agagaaggct gtggaaaaac attacaagag tcttccaggt gaaagcaaga
     2101 caaagtttca tactcaagaa caagtggtca gaagtctgac aggcagtggc agtcaggagg
     2161 acctgctgct tcagaaatcc aatgaaaaag atttggaagc aatacagcag aactgctatt
     2221 taatgactgc agaggagctc aagtttggga gcgatggctt aataacagaa aagtgttctc
     2281 agcagtcacc agacagcaaa ctcatcttct ccaaggaaaa gcaacaatca gagtatgaag
     2341 ggctgactgg ggacttaaaa gcagaacaga acatctatgc ccatctggca aagactcagg
     2401 acacagacag tgtcagcaac ctgcaggctg agctgaaaga ggtccttgcc cttcggaagc
     2461 agctggagca ggatgttctt gcctaccgga acctgcagaa ggccctgcag gagcagctcg
     2521 atgagattcg gagtcgagaa gaagagccgt tttcatttta tagcgatcaa acatcgtatc
     2581 tgagtatttg ccttgaagag cacaatcagt tccaattgga gcatttttct caagaagaac
     2641 tcaagaaaaa ggtcagtgac ctcatacagc ttgtgaagga cctgcacaca gacaaccagc
     2701 acctgaagaa aaccattttt gatctctctt ctgtgggttt ccagggaagt gacagacttg
     2761 agttaacaaa acaagaagag cttgtggcta gcaaggagga tgaggacaca ctcaaatttg
     2821 aagcggacgt tgagactcct ttccagagtg accagcattt ggagcaaagt cgtgagatca
     2881 tggaggacta tgctgagggt ggctgcaaaa gtggatatgg aaggcacatg gattccaata
     2941 ttttaggtca tgatggggcc caaactcctg gtgcctctga agagcacacc ctagaagatg
     3001 agctcctggg cctgcttgcc actctcttca gtaagaaggc cacaccgttg ctggaatcca
     3061 ggccagatct tctgaaagca ctcggtgccc tacttttgga acgcatatgc ttggcagagc
     3121 agggtcgtcc tggagatcac cttgatagta aaactgagaa ggcacttcag caagtagctg
     3181 ttcagttgag agatgaactt ggccattcct tccctgccaa ctcgttctcc aagtcataca
     3241 atgaagtcaa gtcaatgtgg ggaaattggc tggtaaagac aggagacgaa gacacggtgg
     3301 agctgaagag tgtttcagtg cagaccatgg ccatagagga caccccgcat ggattcaaac
     3361 ctcaaagcaa gagagatgct tgggcagaga aacaagaaga ggccattttc agcactgagc
     3421 ttgagtcaga ggcgttgggt gagatgccag ggcaacaggc cactcacctc tcattcccca
     3481 gtgccatcaa cccggatgcc gagaagacgg gcttgttgat ccaattaaag acacctgagt
     3541 tattggaaaa tttatataat cttcctgctt cgccagaggt ggtggtggct caactccaag
     3601 gccaggttct agagctgcag agggagctga aagaattcaa aacccgcaac aagcaactcc
     3661 atgagaagct aattctggca gaggcaatga tggaggggct gccggtgccc aactctgcgc
     3721 tggtgaatgt gcctgcagcc caggctgttg tgagaacagc cttccaggac aacccaggcg
     3781 agcaagaagg acctgaaacc acacagtctg ctgggagaga caaggacatg gacagtgacc
     3841 aatatacaag ctttgagatc gattctgaaa tctgtccacc tgatgacctt gccttattac
     3901 cagcatgcaa agagaatctt gaagacctcc taggcccatc ctccatagcc acttacctgg
     3961 attccaagag tcagctctct gttaaagtca gcgtgaatgg aactgaccag tctgagaata
     4021 tcaatatccc tgatgacaca gaagacttaa aacagaaaat ccacgacttg caaaccgagc
     4081 tggaaggcta ccggaacatc attgtccagc tactgaagca ctctcagtgc agtgaggcta
     4141 ttatcacagt tttgtgtggg acagaagggg cccaagatgg cttgaacaag cct
//
