LOCUS       sequence-201726-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid encoding MmPylRS(IPYE) and pylT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR
  TITLE     Continuous directed evolution of aminoacyl-tRNA synthetases.
  JOURNAL   Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.
  PUBMED    29035361
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474."
COMMENT     Sequence Label: pTECH-MmPylRS(IPYE)
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
       61 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
      121 aaagcaaatt cgacccgggc ggccgcaacc cagcgttcga tgcttctttg agcgaacgat
      181 caaaaataag tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac
      241 ttgtaacgct acatggagat taactcaatc tagagggtaa ctagtatgga taaaaaacca
      301 ctaaacactc tgatatctgc aaccgggctc tggatgtcca ggaccggaac aattcataaa
      361 ataaaacacc acgaaatctc tcgaagcaaa atctatattg aaatggcatg cggagaccac
      421 cttgttgtaa acaactccag gagcagcagg cctgcaagag cgctcaggta ccacaaatac
      481 aggaagacct gcaaacgctg cagggtttcg gatgaggatc tcaataagtt cctcacaaag
      541 gcaaacgaag accagacaag cgtaaaagtc aaggtcgttt ctgaacctac cagaacgaaa
      601 aaggcaatgc caaaatccgt tgcgagagcc ccgaaacctc ttgagaatac agaagcggca
      661 caggctcaac cttctggatc taaattttca cctgcga
//
