LOCUS       sequence-201767-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that 
            targets the FWA promoter in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_TET1cd
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..72
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             100..123
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atggttgggt accaacaaat ggctggtgga cttaacgata tcttcgaagc tcagaagatt
       61 gaatggcatg aggatactgg tggatctagc atccccggac ttgaggttct tttccaagga
      121 cctctcgagc cttataaatg ccctgagtgt ggaaaatctt tctctcaatc tggagatctt
      181 aggaggcacc aaaggaccca taccggtgaa aaaccttata aatgccctga gtgtggaaaa
      241 tcattcagta ggagtgatca tcttaccacc catcagagga cccatactgg tgaaaaacct
      301 tataaatgcc ctgagtgtgg aaaatcattc tcacaatctt catctcttgt taggcatcag
      361 aggactcata ccggagaaaa accttataaa tgccctgagt gtggaaaatc attctcaaca
      421 tctggaaacc ttgttaggca ccaaaggacc catactggtg aaaaacctta taaatgccct
      481 gagtgtggaa aatcattctc tcagagagca aaccttaggg ctcatcaaag gacacacact
      541 ggagaaaaac cttataaatg ccctgagtgt ggaaaatctt tctctaggag tgataagctc
      601 accgagcatc aaaggactca caccggac
//
