LOCUS       sequence-201770-        4368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter 
            in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_YPet
FEATURES             Location/Qualifiers
     source          1..4368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2022..2066
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2094..2117
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2628..2699
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2739..2763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2814..2816,2817..3530)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYN"
     primer_bind     complement(3117..3136)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(3547..3571)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtctagctc aacagagctt ttaacccaaa ttggtacaat agaatacaac tttagatcat
       61 aattctcaaa agaaagagat tccttagcta ttctatctgc cactccattt ccttctcggc
      121 ttgtatgcac aagcataaaa tcctcaaact tgctaagtag atactttatg tcttggataa
      181 ttggattgag acttgacaag cataactttc atgtaaccaa agacacaagt tgctgagaat
      241 ccacctcaaa aatgatcttc ctataattga atcgggataa tgacagcaca gcccatctaa
      301 gagcctccac ttctacttcc agcacgcttc ttacttttac cacagctctt gcacctaacc
      361 ataacacctt ccctgtatga tcgcgaagca cccaccctaa gccacatttt aatccttctg
      421 ttggccatgc cccatcaaag ttgcacttaa cccaagattg tggtggagct tcccatgttt
      481 ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta cattctgagc ctctttcctt
      541 ctaatccact catctgcatc ttcttgtgtc cttactaata cctcattggt tccaaattcc
      601 ctccctttaa gcaccagctc gtttctgttc ttccacagcc tcccaagtat ccaagggact
      661 aaagcctcca cattcttcag atcaggatat tcttgtttaa gatgttgaac tctatggagg
      721 tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca
      781 aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca
      841 ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg
      901 aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt
      961 tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc
     1021 aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag
     1081 ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa
     1141 aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc
     1201 aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa
     1261 aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg
     1321 atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa
     1381 gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct
     1441 caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca
     1501 cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc
     1561 ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa
     1621 ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca
     1681 aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc
     1741 aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg
     1801 ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt
     1861 tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga
     1921 tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat
     1981 taacagacgc gtccatggtt gggtaccaac aaatggctgg tggacttaac gatatcttcg
     2041 aagctcagaa gattgaatgg catgaggata ctggtggatc tagcatcccc ggacttgagg
     2101 ttcttttcca aggacctctc gagccttata aatgccctga gtgtggaaaa tctttctctc
     2161 aatctggaga tcttaggagg caccaaagga cccataccgg tgaaaaacct tataaatgcc
     2221 ctgagtgtgg aaaatcattc agtaggagtg atcatcttac cacccatcag aggacccata
     2281 ctggtgaaaa accttataaa tgccctgagt gtggaaaatc attctcacaa tcttcatctc
     2341 ttgttaggca tcagaggact cataccggag aaaaacctta taaatgccct gagtgtggaa
     2401 aatcattctc aacatctgga aaccttgtta ggcaccaaag gacccatact ggtgaaaaac
     2461 cttataaatg ccctgagtgt ggaaaatcat tctctcagag agcaaacctt agggctcatc
     2521 aaaggacaca cactggagaa aaaccttata aatgccctga gtgtggaaaa tctttctcta
     2581 ggagtgataa gctcaccgag catcaaagga ctcacaccgg actcgaggac tacaaagacg
     2641 atgacgacaa agactacaaa gacgatgacg acaaagacta caaagacgat gacgacaaaa
     2701 agcttgatat cgccatgggg ggcgcgccaa gctatcaaac aagtttgtac aaaaaagcag
     2761 gctccgcggc cgcccccttc accctagacc ctttgcctgc tgctgctgcc gctgtgagca
     2821 aaggcgaaga gctgttcacc ggcgtggtgc ccatcctggt ggagctggac ggcgacgtga
     2881 acggccacaa gttcagcgtg agcggcgagg gcgagggcga cgccacctac ggcaagctga
     2941 ccctgaagct gctgtgcacc accggcaagc tgcccgtgcc ctggcccacc ctggtgacca
     3001 ccctgggcta cggcgtgcag tgcttcgccc ggtaccccga ccacatgaag cagcacgact
     3061 tcttcaagag cgccatgccc gagggctacg tgcaggagcg gaccatcttc ttcaaggacg
     3121 acggcaacta caagacccgg gccgaggtga agttcgaggg cgacaccctg gtgaaccgga
     3181 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac aagctggagt
     3241 acaactacaa cagccacaac gtgtacatca ccgccgacaa gcagaagaac ggcatcaagg
     3301 ccaacttcaa gatccggcac aacatcgagg acggcggcgt gcagctggcc gaccactacc
     3361 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     3421 accagagcgc cctgttcaag gaccccaacg agaagcggga ccacatggtg ctgctggagt
     3481 tcctgaccgc cgccggcatc accgagggca tgaacgagct ctataattag aagggtgggc
     3541 gcgccgaccc agctttcttg tacaaagtgg ttcgataatt ccttaattaa ctagttctag
     3601 agcggccgcc accgcggtgg agctcctagg tgagtctaga gagttaatta agacccggga
     3661 ctagtcccta gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt
     3721 gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac
     3781 cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata
     3841 atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga
     3901 aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac
     3961 aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa
     4021 cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat
     4081 ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc
     4141 ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta
     4201 cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa
     4261 ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca
     4321 gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacgagct
//
