LOCUS       sequence-201774-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter 
            in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_YPet
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(31..33,34..747)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYN"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctagaccctt tgcctgctgc tgctgccgct gtgagcaaag gcgaagagct gttcaccggc
       61 gtggtgccca tcctggtgga gctggacggc gacgtgaacg gccacaagtt cagcgtgagc
      121 ggcgagggcg agggcgacgc cacctacggc aagctgaccc tgaagctgct gtgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctg gtgaccaccc tgggctacgg cgtgcagtgc
      241 ttcgcccggt accccgacca catgaagcag cacgacttct tcaagagcgc catgcccgag
      301 ggctacgtgc aggagcggac catcttcttc aaggacgacg gcaactacaa gacccgggcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccggatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg
      481 tacatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccggcacaac
      541 atcgaggacg gcggcgtgca gctggccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagctacc agagcgccct gttcaaggac
      661 cccaacgaga agcgggacca catggtgctg ctggagttcc tgaccgccgc cggcatcacc
      721 gagggcatga acgagctcta taattag
//
