LOCUS       sequence-20181-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Logan M, Tabin C
  TITLE     Targeted gene misexpression in chick limb buds using avian 
            replication-competent retroviruses.
  JOURNAL   Methods. 1998 Apr . 14(4):407-20.
  PUBMED    9608511
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            1998 Apr . 14(4):407-20."
COMMENT     Sequence Label: pSLAX13 (CT#232)
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(119..135)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(120..136)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(164..183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(165..183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(190..207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(190..206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      348..728
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(435..454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     645..666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgattctaga ccactgtggc caggcggtag ctgggacgtg cagccgacca ccatggccat
       61 gattacgaat tcgagctcgc ccggggatcc tctagagtcg acctgcagcc aagcttatcg
      121 ataccgtcga cctcgatcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
      181 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cga
//
