LOCUS       sequence-201828-       10314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Lenz DR, Gunewardene N, Abdul-Aziz DE, Wang Q, Gibson TM, Edge ASB
  TITLE     Applications of Lgr5-Positive Cochlear Progenitors (LCPs) to the 
            Study of Hair Cell Differentiation.
  JOURNAL   Front Cell Dev Biol. 2019 Feb 19;7:14. doi: 
            10.3389/fcell.2019.00014. eCollection 2019.
  PUBMED    30873406
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2019-02-19"; volume: "7:14. doi"; pages: " 
            10.3389/fcell.2019.00014. eCollection 2019"
COMMENT     Sequence Label: pLV U6-sgRNA UbC-GFP
FEATURES             Location/Qualifiers
     source          1..10314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2599..2632)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2744..2992)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2802..2821)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2972..2992)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3023..4233
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4070..4089
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4262..4264,4265..4267,4268..4981)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4262..4264,4265..4267,4268..4978)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4307..4328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4568..4587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4915..4936
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5006..5594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5059..5079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5477..5488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5597..5613)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5598..5614)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6119..6299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6325..6342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6331..6555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6601..7029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6688..6707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6898..6919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7038..7058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7043..7372
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7223..7358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7285..7304
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7420..7467
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7486..7860
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7990..8111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8027..8046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8081..8100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8160..8176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8160..8176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8173..8195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8184..8200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8208..8214,8215..8232,8233..8238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8253..8274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8391..8408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8562..9150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8642..8661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9321..10112,10113..10181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9944..9963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10182..10286)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttataat aacttcgtat agcatacatt atacgaagtt attaaaaaaa
     2641 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct
     2701 atgctgtttc cagcatagct cttaaactga gacgtccgtc tccggtgttt cgtcctttcc
     2761 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
     2821 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
     2881 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa
     2941 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttaattat
     3001 aacccgtgtc ggctccagat ctggcctgcg cgccgggttt tggcgcctcc cgcgggcgcc
     3061 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     3121 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     3181 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     3241 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     3301 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     3361 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     3421 gtgagttgcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     3481 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     3541 gaactggggg ttggggggag cgcacaaaat ggcggctgtt cccgagtctt gaatggaaga
     3601 cgcttgtaag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     3661 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     3721 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcgggtttgt
     3781 cgtctggttg cgggggcggc agttatgcgg tgccgttggg cagtgcaccc gtacctttgg
     3841 gagcgcgcgc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     3901 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     3961 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     4021 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     4081 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     4141 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     4201 aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag
     4261 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4321 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4381 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4441 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4501 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4561 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4621 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4681 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4741 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4801 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4861 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4921 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4981 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     5041 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5101 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5161 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5221 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5281 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5341 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5401 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5461 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5521 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5581 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     5641 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5701 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5761 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5821 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5881 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5941 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6001 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6061 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6301 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6361 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6421 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6481 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6541 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7021 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7201 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7321 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7381 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7441 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7501 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7561 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7621 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7681 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7741 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7801 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7861 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7921 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7981 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8101 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8221 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8521 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8581 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8641 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8701 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8761 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8821 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8881 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8941 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9001 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9061 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9121 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9301 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9361 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9421 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9481 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9541 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9601 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9661 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9721 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9781 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9841 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9901 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9961 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10021 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10081 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10141 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10201 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10261 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
