LOCUS       sequence-20186-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Mena MA, Treynor TP, Mayo SL, Daugherty PS
  TITLE     Blue fluorescent proteins with enhanced brightness and 
            photostability from a structurally targeted library.
  JOURNAL   Nat Biotechnol. 2006 Dec . 24(12):1569-71.
  PUBMED    17115054
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2006 Dec . 24(12):1569-71."
COMMENT     Sequence Label: pB18cmBFPAzurite
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..72)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(73..786)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Mena et al., 2006)"
                     /label=Azurite BFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FRTAAGITHGMDELYK"
ORIGIN
        1 catgcctgca ggtcgactct agaggatccc cgggtaccgg ccaccttggc cttagtggtg
       61 gtggtggtgg tgtttgtaca attcatccat accatgggta ataccagcag cagtcctaaa
      121 ttctaacagg accatgtggt ctctcttttc gtttggatct ttggataagg ctgattgggt
      181 ggataagtaa tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg
      241 ataatggtca gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttcac
      301 tttgatacca ttcttttgtt tgtcagccat gatgtatata ttgtgagagt tgaagttgta
      361 ttccaatttg tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat
      421 tctattaact aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc
      481 atctttgaaa aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa
      541 gtcatgttgt ttcatatgat ctgggtatct agaaaaacat tgaacaccat ggctcaaagt
      601 agttactaag gttggccatg gaactggcaa tttaccagta gtacaaataa attttaaggt
      661 caatttaccg tacgtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatt
      721 aacatcacca tctaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt
      781 agacatcgtt tcactcctgg ccttcgtggc cgagctcgaa
//
