LOCUS       sequence-201887-       12020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DDX4(1-236) fused to mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP
  TITLE     Spatiotemporal Control of Intracellular Phase Transitions Using 
            Light-Activated optoDroplets.
  JOURNAL   Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29.
  PUBMED    28041848
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2017-01-12"; volume: "168(1-2):159-171.e14. doi"; pages: " 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29"
COMMENT     Sequence Label: pHR-DDX4N-mCh-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2232..3725
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3877..4465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3930..3950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4348..4359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4468..4484)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4469..4485)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4575..4808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4833..4851)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4834..4851)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5141..5160)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5374..5393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5493..5515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5553..5571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5639..5743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5744..5812,5813..6604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5962..5981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6775..7363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7264..7283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7517..7534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7604..7624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7609..7938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7789..7924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9072..9137
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9267..9287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9712..9846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9749..9768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9803..9822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10014..10647
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10694..10819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11316..11549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11734..11778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggga gatgaagatt gggaagcaga aatcaaccct catatgtctt
      841 cctatgttcc catatttgag aaggataggt attctggaga aaatggagac aattttaaca
      901 ggactccagc ttcatcatca gaaatggatg atggaccttc tcgaagagat catttcatga
      961 aaagtggatt tgcctctggg cggaattttg gaaacagaga tgctggtgag tgtaataagc
     1021 gagataatac atccacaatg ggtggttttg gagttggaaa gagttttgga aacagaggtt
     1081 tttcaaacag caggtttgaa gatggtgata gctctggttt ctggagagag tctagtaatg
     1141 actgcgaaga taatccaaca cggaacagag ggttttccaa gagaggcggc tatcgagatg
     1201 gaaataattc agaagcttca gggccataca gaagaggtgg aagaggtagt ttccgaggtt
     1261 gccgtggagg atttggtcta ggaagtccaa ataatgactt agacccagac gaatgtatgc
     1321 agcgcactgg tggccttttt ggttctagaa gaccagtatt aagtggcaca ggtaatggtg
     1381 atacttctca aagcagaagt ggcagtggaa gtgaacgagg tggttacaaa ggtttaaatg
     1441 aagaagtaat aacaggctct ggaaagaatt cttggaagtc agaagcagaa ggaggagaaa
     1501 gtggcggcgg catggtgtct aaaggcgagg aggataacat ggctataatc aaggaattta
     1561 tgaggttcaa ggtgcatatg gagggatctg tgaacggtca cgagttcgaa atcgagggcg
     1621 aaggcgaggg gcgaccctat gaagggacac agacggccaa actgaaggtg accaagggtg
     1681 gccccctgcc tttcgcctgg gacatcctgt caccccagtt tatgtacggc tctaaggctt
     1741 acgttaagca ccctgccgat attccggact acttgaaact gagcttccct gaagggttca
     1801 agtgggaacg ggtaatgaat tttgaggatg gcggtgtagt cacagttacc caggacagct
     1861 ctcttcagga cggagaattt atctataagg ttaaacttcg gggcactaac ttcccatcag
     1921 acggccccgt gatgcagaag aaaactatgg gctgggaagc cagctcagaa cgcatgtatc
     1981 ccgaggacgg agcactgaag ggagaaatca agcagcgact caagctgaag gatgggggac
     2041 attatgatgc tgaggtcaaa accacctata aggccaagaa acccgttcag cttcctgggg
     2101 catacaacgt gaatatcaaa ctggatatta catcccataa cgaagactat accatcgtgg
     2161 aacagtacga gcgggccgag ggcagacata gcacaggggg catggatgaa ttgtacaagg
     2221 cggccacgcg tatgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
     2281 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
     2341 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
     2401 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
     2461 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
     2521 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
     2581 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
     2641 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
     2701 aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa
     2761 taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg ctggagaatg
     2821 aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga tggagcaatg
     2881 ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca aagaacagca
     2941 agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc ggggaaataa
     3001 gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca agagataaga
     3061 acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta agagagtatt
     3121 ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg agtcatcttc
     3181 ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa ggcaggaccg
     3241 gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg atgcataaca
     3301 gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca tggaaatggg
     3361 gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac atccttggct
     3421 ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg gacaatcccg
     3481 cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg cttcccgagc
     3541 ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta accgtactca
     3601 aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac atcgacacag
     3661 ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc atgatcggag
     3721 cagcagcccg ggatccaccg gtcgccaccg gttcagggag cggatccggc tcagggagtg
     3781 cggccgcaac tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac
     3841 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     3901 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3961 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4021 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4081 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4141 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4261 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4321 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4381 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4441 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag
     4501 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4801 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     4861 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     4921 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     4981 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     5041 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     5101 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     5161 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     5221 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     5281 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     5341 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5401 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5461 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5521 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5581 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5761 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5881 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5941 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6001 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6061 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7441 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7501 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7561 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     7621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7921 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     7981 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     8041 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     8101 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     8161 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     8221 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     8281 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     8341 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     8401 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     8461 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     8521 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     8581 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     8641 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     8701 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     8761 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     8821 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
     8881 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
     8941 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
     9001 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     9061 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     9121 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     9181 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     9241 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     9301 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     9361 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     9421 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
     9481 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
     9541 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
     9601 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     9661 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     9721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9841 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     9901 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     9961 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
    10021 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
    10081 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
    10141 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
    10201 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
    10261 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
    10321 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
    10381 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
    10441 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10501 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10561 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10621 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
    10681 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
    10741 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
    10801 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
    10861 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
    10921 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
    10981 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
    11041 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
    11101 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
    11161 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
    11221 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    11281 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    11341 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    11401 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    11461 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    11521 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    11581 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    11641 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    11701 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    11761 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    11821 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    11881 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    11941 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    12001 agatccattc gattagtgaa
//
