LOCUS       sequence-20190-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pCEP4YPet-MAMM
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(377..396)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctcgttta gtgaaccgtc agatctctag aagctgggta ccgcgggccc gggatccacc
       61 ggtcgccacc atggtgagca aaggcgaaga gctgttcacc ggcgtggtgc ccatcctggt
      121 ggagctggac ggcgacgtga acggccacaa gttcagcgtg agcggcgagg gcgagggcga
      181 cgccacctac ggcaagctga ccctgaagct gctgtgcacc accggcaagc tgcccgtgcc
      241 ctggcccacc ctggtgacca ccctgggcta cggcgtgcag tgcttcgccc ggtaccccga
      301 ccacatgaag cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggagcg
      361 gaccatcttc ttcaaggacg acggcaacta caagacccgg gccgaggtga agttcgaggg
      421 cgacaccctg gtgaaccgga tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      481 cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca ccgccgacaa
      541 gcagaagaac ggcatcaagg ccaacttcaa gatccggcac aacatcgagg acggcggcgt
      601 gcagctggnc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      661 cgacaaccac tacctgagct accagagcgn cctgttcaag gaccccaacg agaagcggga
      721 ccacatggng ctgctggagt tcctgaccgc cgnc
//
