LOCUS       sequence-201907-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses epitope-tagged double-mutant murine TTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C, 
            Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders 
            AP, Lai WS, Blackshear PJ, Downward J
  TITLE     Oncogenic RAS Signaling Promotes Tumor Immunoresistance by 
            Stabilizing PD-L1 mRNA.
  JOURNAL   Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 
            10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.
  PUBMED    29246442
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2017.11.016"; journalName: "Immunity"; date: 
            "2017-12-19- 19"; volume: "47"; issue: "6"; pages: "1083-1099.e6"
COMMENT     Sequence Label: pTRIPZ Myc2-mouse TTP aa S52 S178 MUT
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             34..63
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1024..1035)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggagcaga agctgatctc agaggaggat ctggagcaga agctgatctc agaggaggat
       61 ctggatatcc agcacagtgg cggccgcgat ctctctgcca tctacgaggt gagtacctgt
      121 tagacagcat cccgggatcc ccgacgcacc aaacttaggc ccgggacgcc tccgtacctt
      181 ctcagactag ccctggctgg ctggaaatga gagagggagc cagcgagcct agcgcctcgg
      241 tccagatacc ggcgatcccc ttgggacgaa aagctggcac actgctgtca ctcgcgtagg
      301 gcggaagcgg catcacacct gtctagtgcc cttcgctgcc catggcgcgg tttggtggcc
      361 aagcaggtgc taaggttgag gtaggggggg agttgacagg gtaagagttc tagagagatc
      421 tggcctcctg ggtcagcttt ggccggctcc ttcaagttgt gaaacgagcg cccggtgggc
      481 ggtcatatgg gggggggggg agtcctagag gcccaaagtt ggcgagcagc agtgtgcttt
      541 gatttggggg ggaaccctct ctcgatcggg gatactgggc ttcctagatc cttctggatt
      601 accttcagtc atgtggaaac actcaaatct ctgtagtcag gttctccctg gagtttgtgt
      661 gagccgggtg gggctcaggc cgagaaccca caaaccggac tggcaagctc gtgaagtgct
      721 ttacaacagg ggcggggcga cgtcacctta ccttttctgg tggcctaacc gaaccctgtt
      781 cctctcagag cctccagtcg atgagccatg acctgtcatc cgaccacgga ggaaccgaat
      841 ccctcggagg actttggaac ataaactcgg actccatccc gtctggggtc acctctcgcc
      901 tgactggccg ctccactgcc ctggtggagg gccgaagctg tggctgggta cccccacccc
      961 ctggttttgc acctttggct ccccgcccag gccctgagct gtcaccctca cctacttcgc
     1021 ctactgcaac tcccaccacc tcctctcgat acaagaccga gctctgtcgg acctactcag
     1081 aaagcgggcg ttgtcgctac ggggccaagt gccagtttgc tcacggcctg ggtgaacttc
     1141 gccaagccaa tcgccacccc aagtacaaaa cggaactctg ccacaagttc tacctccagg
     1201 gccgctgccc ctatggctct cgatgccact tcatccacaa ccccaccgag gacctagctc
     1261 tccctggcca gccccatgtg ctgcgacaaa gcatcgcctt ctccggcttg ccctcaggcc
     1321 gcagaagctc gccgccacct ccaggctttt ctggcccttc cctgtcctct tgttcctttt
     1381 cgccttccag ctccccaccg ccccctgggg atcttccact ttccccttct gccttctctg
     1441 ctgcccctgg gacccctgtg actcgaagag accctaacca ggcctgttgc ccctcctgcc
     1501 gaaggtctac tacccccagc accatctggg ggcccttggg tggcctggct cggagcccat
     1561 ctgcccactc tctgggatcc gatcctgatg actacgccag cagcggcagc agcctggggg
     1621 ggtcagactc acctgtcttt gaggcagggg tgtttgggcc tccccagacc cctgcacccc
     1681 caaggcgtct ccccatcttc aatcgtatct ctgtctctga gtga
//
