LOCUS       sequence-20191-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pCEP4CyPet-MAMM
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(60..62,63..65,66..779)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(366..385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(808..827)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtgaccgtca gatctctaga agctgggtac cgcgggcccg ggatccaccg gtcgccacca
       61 tggtgagcaa gggagaggaa ctgttcggcg gcatcgtgcc catcctggtg gagctggagg
      121 gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg cgagggcgac gccacctacg
      181 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tggtgaccac cctgacctgg ggcgtgcagt gcttcagccg gtaccccgac cacatgaagc
      301 agcacgactt cttcaagagc gtgatgcccg agggctacgt gcaggagcgg accatcttct
      361 tcaaggacga cggcaactac aagacccggg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccggat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctgggccaca
      481 agctggagta caactacatc agccacaacg tgtacatcac cgccgacaag cagaagaacg
      541 gcatcaaggc caacttcaag gcccggcaca acatcaccga cggcagcgtg cagctggccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gatcctgccc gacaaccact
      661 acctgagcac ccagagcgcc ctgagcaagg accccaacga gaagcgggac cacatggtgc
      721 tgctggagtt cgtgaccgcc gccggcatca cccacggcat ggacgaactg tacaaataat
      781 aaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      841 tgaaaaaaat gctttatttg
//
