LOCUS       sequence-20193-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Brent AE, Tabin CJ
  TITLE     FGF acts directly on the somitic tendon progenitors through the Ets 
            transcription factors Pea3 and Erm to regulate scleraxis expression.
  JOURNAL   Development. 2004 Aug . 131(16):3885-96.
  PUBMED    15253939
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2004 Aug . 131(16):3885-96."
COMMENT     Sequence Label: pBS cFgfr3 (CT#219)
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(541..557)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(542..558)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(587..607)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(587..605)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(626..642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(626..642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(673..679,680..697,698..703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    718..739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgattg gagcttggtg
       61 atggagagcg ttgtgccgtc agatcgagga aactacacct gtgttgtgga gaacaaatat
      121 ggcaatatta ggcacacata ccagcttgat gttttagaac ggtcacccca ccgaccaatc
      181 ctgcaagcag gactccctgc caatcagact gtggtggtcg ggagcaatgt ggaatttcac
      241 tgcaaggtct acagcgatgc ccagcctcat atccagtggc tgaaacacgt ggaagtcaac
      301 ggcagcaagt atggacctga tgggacaccc tatgtcacag tgctgaagac ggcaggtgtt
      361 aacacaacgg ataaggagct agagattctg tacttgcgaa atgttacttt tgaggatgct
      421 ggggaatata cttgtctcgc agggaattct attgggttct cacatcactc tgcttggctg
      481 acggtgctac cagcagagga gctgatggaa atggatgatt cgggctcaga tcaagcttat
      541 cgataccgtc gacctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt
      601 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgnnnt gttatccgct
      661 cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg
      721 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cng
//
