LOCUS       sequence-20195-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Logan M, Tabin C
  TITLE     Targeted gene misexpression in chick limb buds using avian 
            replication-competent retroviruses.
  JOURNAL   Methods. 1998 Apr . 14(4):407-20.
  PUBMED    9608511
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            1998 Apr . 14(4):407-20."
COMMENT     Sequence Label: pSLAX13 Flag (CT#231)
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     46..62
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(86..109)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(209..227)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(248..264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(248..264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(261..283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(296..302,303..320,321..326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    341..362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(479..496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tacgactcac tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat
       61 cgattctaga ctgcagttag gatcccttgt catcgtcgtc cttgtagtcg ctagcggaat
      121 tcgtaatcat ggccatggtg gtcggctgca cgtcccagct accgcctggc cacagtggtc
      181 tagaatcgat gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
      241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      361 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      421 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      541 aggcggtaat acggttatcc acagaatcag gggataacgc agaaagaaca tgtgagcaaa
      601 agccagcaaa aggcca
//
