LOCUS       sequence-201959-       13444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal
            super-folder GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: K785R Smarca4-sfGFP
FEATURES             Location/Qualifiers
     source          1..13444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3708..3752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4019..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4186..4565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4568..4843
                     /label=chicken beta-actin promoter
     primer_bind     5776..5798
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(10763..10765,10766..11479)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10805..10826)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     11413..11434
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    11510..12098
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11563..11583)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11981..11992)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12185..12418
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12490..12611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12527..12546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12581..12600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12630..12765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12797..12816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12798..12816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12826..12843)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12826..12842)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12835..12857)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12987..13442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13074..13093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13284..13305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgatc ggccacgcgg atctcgacat tgattattga
     4201 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4261 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4321 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4381 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4441 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4501 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4561 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     4621 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     4681 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     4741 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     4801 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     4861 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     4921 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     4981 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     5041 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     5101 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     5161 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     5221 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     5281 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     5341 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     5401 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5461 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     5521 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5581 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     5641 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     5701 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     5761 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctccgga gcgcacgtcg
     5821 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccgggc
     5881 tagcatcgcc accatgtcta ctccagaccc acccttgggt gggactcctc ggcctggtcc
     5941 ttccccaggc cctggtcctt cacctggtgc aatgctgggt cctagccctg gcccctcacc
     6001 aggttctgcc cacagcatga tggggccaag cccaggacct ccttcagcag gacatcccat
     6061 gcccacccag gggcctggag ggtaccccca ggacaacatg catcagatgc acaagcctat
     6121 ggagtccatg cacgagaagg gcatgcctga tgacccacga tacaaccaga tgaaagggat
     6181 gggcatgcgg tcaggggccc acacaggcat ggcacctcca cctagtccca tggaccagca
     6241 ttctcaaggt tacccctcac ccctcggcgg ctctgaacat gcctccagtc ctgtcccagc
     6301 cagtggccca tcttcaggcc cccagatgtc ctctgggcca ggaggggccc cactagatgg
     6361 ttctgatccc caggccttgg gacagcaaaa cagaggccca accccattta accagaacca
     6421 gctgcatcaa ctcagagctc agataatggc ctacaagatg ttggccaggg gccagccatt
     6481 gcccgaccac ctgcagatgg ccgtgcaagg caagcggccg atgcctggaa tgcagcaaca
     6541 gatgccaaca ctacctccac cctcagtgtc cgccacagga cccggacctg gacccggccc
     6601 tggccctggc cctggcccag gaccagcccc tccaaattac agtagacccc atggtatggg
     6661 agggcccaac atgcctcccc caggaccctc aggtgtgccc cccgggatgc ctggtcagcc
     6721 gcctggaggg cctcccaagc catggcctga aggacccatg gccaatgctg ctgcccccac
     6781 aagcacccca cagaagctga ttcctccgca accaacaggc cgtccttcac ctgcacctcc
     6841 tgctgtcccg cctgctgcct cacctgtaat gccaccacaa acacagtccc cagggcagcc
     6901 agcccagcct gctccattgg tgccactgca ccagaagcag agccgaatca cccccatcca
     6961 gaagccccga ggccttgacc ctgtggagat cctacaagag cgggagtaca ggcttcaggc
     7021 tcgaatcgca cacagaattc aggaacttga aaacctccct gggtccctgg ctggggacct
     7081 tcgaaccaaa gcaaccatcg aactcaaggc ccttaggttg ctgaacttcc agaggcagct
     7141 gcgccaggag gtggtggtgt gcatgcgaag agacacagcc ctggagacag ccctcaatgc
     7201 caaggcctac aagcgcagca aacgtcagtc actacgggag gcccgcatca ctgagaagtt
     7261 ggagaagcag cagaagattg aacaggagcg caagcgccgc cagaagcacc aggagtacct
     7321 caacagcatt ctgcagcatg ccaaggactt cagggagtat cacagatcag tcacaggcaa
     7381 actccagaaa ctcaccaagg ctgtggccac ctaccatgcc aacactgagc gggagcagaa
     7441 gaaagaaaat gagcgcattg agaaggagcg aatgcggagg cttatggctg aagatgagga
     7501 gggctaccgc aaactcattg accagaagaa ggacaagcgc ctggcctacc ttctgcagca
     7561 gacagatgag tatgtggcca acctcacaga gctggtgcgg cagcacaaag ctgcccaggt
     7621 tgccaaggag aagaagaaga aaaagaaaaa gaagaaggca gaaaatgctg aaggacagac
     7681 acctgctatt ggaccagatg gtgagcctct ggatgagacc agccagatga gtgacctccc
     7741 tgtgaaggtg atccacgtgg agagtggcaa gatcctcact ggcacagatg ccccaaaagc
     7801 cgggcagctg gaagcctggc ttgaaatgaa cccagggtat gaagtagccc ccaggtcaga
     7861 cagtgaagaa agtggctctg aagaggagga ggaggaggag gaagaggagc agcctcagcc
     7921 cgcacagccc cctacactgc ctgtggaaga aaagaagaag attccagacc cagacagcga
     7981 tgatgtctct gaggtggacg cccgacacat tattgagaac gccaagcaag atgtggacga
     8041 tgagtacggt gtgtcccagg cccttgctcg tggcctgcag tcttactatg ctgtggccca
     8101 tgcagtcaca gagagagtag ataagcagtc cgccctcatg gtcaacggtg tcctcaaaca
     8161 gtaccagatc aagggtttgg agtggctggt gtccctgtac aacaacaacc tgaatggcat
     8221 cctggctgat gagatggggc tggggaggac catccagacc atcgcgctca tcacatacct
     8281 catggagcac aagcgcatca acgggccttt cctcatcatc gtgcctctct cgacactgtc
     8341 aaactgggcg tatgaatttg acaagtgggc cccctctgtg gtgaaggttt cttacaaggg
     8401 ctctccagct gcaaggcgag cttttgtccc acagcttcgc agtgggaagt tcaacgtctt
     8461 actgaccacc tatgaatata tcatcaaaga caagcatatc ctagccaaga tccgctggaa
     8521 gtacatgatt gtggatgaag gccaccgcat gaaaaaccac cactgcaagt tgacgcaggt
     8581 ccttaacaca cactacgtgg cccctcggcg cctgcttctt acaggcacac cactgcagaa
     8641 caagctaccg gagctctggg ccctgcttaa cttcctgctc cccactatct tcaagagctg
     8701 cagcaccttc gaacagtggt tcaatgcacc ctttgccatg actggagaaa aggtggacct
     8761 gaatgaagag gagactatcc tcattattcg tcgcctacac aaagttctgc ggcccttcct
     8821 gctgcggcgg ctcaagaagg aagttgaagc ccagctccct gagaaggtag agtatgtcat
     8881 caaatgcgac atgtcagccc tgcagcgtgt gctgtaccgt cacatgcagg ccaaaggtgt
     8941 gctgctgact gacggctccg agaaggacaa gaagggcaaa ggtggcacca agacactgat
     9001 gaacactatt atgcaactgc gtaagatctg caaccacccc tacatgttcc agcacatcga
     9061 ggagtccttt tctgagcact tggggttcac cggcggcatc gtgcaaggat tggaccttta
     9121 ccgtgcctca gggaaatttg aacttcttga tagaattcta cccaaactcc gtgcaacgaa
     9181 ccataaagtg ctcctctttt gccaaatgac ctccctcatg accatcatgg aagactactt
     9241 tgcataccgt ggcttcaaat acctcaggct tgatggaacc acaaaagcag aagaccgggg
     9301 catgctgttg aaaaccttta atgaacctgg ctctgagtat ttcattttcc tgctcagtac
     9361 ccgtgctggg gggctgggcc tgaatctgca gtcagctgac actgtgatca tctttgacag
     9421 tgactggaat ccccaccagg acctgcaagc acaggatcga gcccatcgca ttggacagca
     9481 gaatgaggtg cgtgttcttc gcctgtgcac ggtcaacagt gtggaagaga agatactggc
     9541 tgctgccaaa tacaaactca atgtggatca gaaggtgatc caggcaggca tgttcgacca
     9601 gaagtcgtcc agccatgaga ggcgtgcctt cctgcaggcc atcctggagc acgaggagca
     9661 ggatgaggag gaagatgagg tgcctgatga tgagaccgtc aaccagatga ttgcccggca
     9721 cgaagaagag tttgacctct tcatgcgcat ggacttggac cgccggcgtg aagaagcccg
     9781 caaccccaag cggaagccac gcctgatgga agaggatgag ctcccatcct ggatcatcaa
     9841 ggatgatgcc gaggtggagc ggctgacatg tgaagaggaa gaggagaaga tgttcggccg
     9901 tggttctcgc caccgcaagg aggtagacta cagcgactca ctgacagaga agcagtggct
     9961 caaggctatc gaggagggca cgctggagga gatcgaagag gaggtccggc agaagaaatc
    10021 ttcacgtaag cgtaagcgag acagcgaggc cggctcctcc accccgacca ccagcacccg
    10081 cagccgtgac aaggatgagg agagcaagaa gcagaagaaa cgtgggcggc cacctgctga
    10141 gaagctgtcc ccaaacccac ctaacctcac caagaagatg aagaagatcg tggatgctgt
    10201 gatcaagtac aaagacagca gcagtggacg tcagctcagc gaggtgttca tccagctccc
    10261 ctctcgcaag gagcttcctg agtactatga gctcatccga aagcctgtgg acttcaagaa
    10321 gatcaaggaa cgcatccgaa accacaagta ccgcagcctc aatgacctgg agaaggatgt
    10381 gatgctgctg tgccagaacg ctcagacgtt caacctcgag ggttccctga tctatgagga
    10441 ctccatcgtc ctgcagtctg tcttcaccag cgtacggcag aagattgaga aggaggacga
    10501 cagtgaaggc gaggaaagcg aggaggagga ggagggcgag gaggaaggct ccgagtctga
    10561 gtcccgctcc gtcaaggtga agatcaagct gggccgcaag gagaaggccc aggaccgact
    10621 caaggggggc cgccggcggc caagccgggg atcccgggcc aagccggttg tgagtgacga
    10681 tgacagtgag gaggagcagg aggaggaccg ctcaggaagt ggcagtgagg aagacggtgg
    10741 aaccggtagt ggaggatccg gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
    10801 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg
    10861 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
    10921 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
    10981 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
    11041 ccaggagcgc accatctcct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
    11101 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
    11161 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg tctatatcac
    11221 ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acgtcgagga
    11281 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
    11341 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
    11401 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
    11461 ggacgagctg tacaagtaag tcgacgatat cgtacaagta agattcgaca atcaacctct
    11521 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    11581 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    11641 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    11701 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    11761 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    11821 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    11881 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
    11941 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    12001 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
    12061 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct
    12121 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    12181 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
    12241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    12301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    12361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    12421 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
    12481 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    12541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    12601 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
    12661 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    12721 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg
    12781 agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
    12841 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    12901 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    12961 gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg
    13021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    13081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    13141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    13201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    13261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    13321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    13381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
    13441 ttcc
//
