LOCUS       sequence-20196-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Logan M, Tabin C
  TITLE     Targeted gene misexpression in chick limb buds using avian 
            replication-competent retroviruses.
  JOURNAL   Methods. 1998 Apr . 14(4):407-20.
  PUBMED    9608511
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            1998 Apr . 14(4):407-20."
COMMENT     Sequence Label: pSLAX13 HA (CT#233)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..100
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             161..187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(220..236)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(221..237)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(265..284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(266..284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(291..308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(291..307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(536..555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 anngnctatg attctgacac tgtggccagg cggtagctgg gacgtgcagc cgaccaccat
       61 ggccatgatt acgaattccg ctagcggcat cgagggcaga tacccatacg atgttcctga
      121 ctatgcgggc tatccctatg acgtcccgga ctatgcaggc tatccatatg acgttccaga
      181 ttacgcagga gctcagggat cctaactgca gtctagaatc gataccgtcg acctcgatcg
      241 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      421 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      481 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      541 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      601 cgggggctcc ctttggngtc cgatttagtg cttaacggca cctcgaccca aaaacttgat
      661 nagtnagatg ttcacnatgg cnccncncna naaanggttt tcccctttga gtngancncn
      721 ttttnaaagg atttgtcaaa tgaaanatca ccnttngttt ctttatnaag attgcatcgc
      781 ntgtaaatgc nnttcantna gtttnaatnn attantngtt tgnttggagc gacttttntt
      841 atca
//
