LOCUS       sequence-201961-       14055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with 
            IRES puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 14055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: K785R Smarca4-V5 IRES-puro
FEATURES             Location/Qualifiers
     source          1..14055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3708..3752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4019..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4186..4565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4568..4843
                     /label=chicken beta-actin promoter
     primer_bind     5776..5798
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             10752..10793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    10872..11444
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11039..11056)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11266..11285
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11447..12049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     11946..11966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12121..12709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12174..12194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12592..12603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12796..13029
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13101..13222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13138..13157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13192..13211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13241..13376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13408..13427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13409..13427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13437..13454)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13437..13453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13446..13468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13598..14053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13685..13704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13895..13916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgatc ggccacgcgg atctcgacat tgattattga
     4201 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4261 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4321 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4381 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4441 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4501 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4561 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     4621 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     4681 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     4741 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     4801 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     4861 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     4921 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     4981 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     5041 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     5101 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     5161 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     5221 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     5281 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     5341 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     5401 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5461 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     5521 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5581 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     5641 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     5701 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     5761 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctagc cggagcgcac
     5821 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc
     5881 gggctagcat cgccaccatg tctactccag acccaccctt gggtgggact cctcggcctg
     5941 gtccttcccc aggccctggt ccttcacctg gtgcaatgct gggtcctagc cctggcccct
     6001 caccaggttc tgcccacagc atgatggggc caagcccagg acctccttca gcaggacatc
     6061 ccatgcccac ccaggggcct ggagggtacc cccaggacaa catgcatcag atgcacaagc
     6121 ctatggagtc catgcacgag aagggcatgc ctgatgaccc acgatacaac cagatgaaag
     6181 ggatgggcat gcggtcaggg gcccacacag gcatggcacc tccacctagt cccatggacc
     6241 agcattctca aggttacccc tcacccctcg gcggctctga acatgcctcc agtcctgtcc
     6301 cagccagtgg cccatcttca ggcccccaga tgtcctctgg gccaggaggg gccccactag
     6361 atggttctga tccccaggcc ttgggacagc aaaacagagg cccaacccca tttaaccaga
     6421 accagctgca tcaactcaga gctcagataa tggcctacaa gatgttggcc aggggccagc
     6481 cattgcccga ccacctgcag atggccgtgc aaggcaagcg gccgatgcct ggaatgcagc
     6541 aacagatgcc aacactacct ccaccctcag tgtccgccac aggacccgga cctggacccg
     6601 gccctggccc tggccctggc ccaggaccag cccctccaaa ttacagtaga ccccatggta
     6661 tgggagggcc caacatgcct cccccaggac cctcaggtgt gccccccggg atgcctggtc
     6721 agccgcctgg agggcctccc aagccatggc ctgaaggacc catggccaat gctgctgccc
     6781 ccacaagcac cccacagaag ctgattcctc cgcaaccaac aggccgtcct tcacctgcac
     6841 ctcctgctgt cccgcctgct gcctcacctg taatgccacc acaaacacag tccccagggc
     6901 agccagccca gcctgctcca ttggtgccac tgcaccagaa gcagagccga atcaccccca
     6961 tccagaagcc ccgaggcctt gaccctgtgg agatcctaca agagcgggag tacaggcttc
     7021 aggctcgaat cgcacacaga attcaggaac ttgaaaacct ccctgggtcc ctggctgggg
     7081 accttcgaac caaagcaacc atcgaactca aggcccttag gttgctgaac ttccagaggc
     7141 agctgcgcca ggaggtggtg gtgtgcatgc gaagagacac agccctggag acagccctca
     7201 atgccaaggc ctacaagcgc agcaaacgtc agtcactacg ggaggcccgc atcactgaga
     7261 agttggagaa gcagcagaag attgaacagg agcgcaagcg ccgccagaag caccaggagt
     7321 acctcaacag cattctgcag catgccaagg acttcaggga gtatcacaga tcagtcacag
     7381 gcaaactcca gaaactcacc aaggctgtgg ccacctacca tgccaacact gagcgggagc
     7441 agaagaaaga aaatgagcgc attgagaagg agcgaatgcg gaggcttatg gctgaagatg
     7501 aggagggcta ccgcaaactc attgaccaga agaaggacaa gcgcctggcc taccttctgc
     7561 agcagacaga tgagtatgtg gccaacctca cagagctggt gcggcagcac aaagctgccc
     7621 aggttgccaa ggagaagaag aagaaaaaga aaaagaagaa ggcagaaaat gctgaaggac
     7681 agacacctgc tattggacca gatggtgagc ctctggatga gaccagccag atgagtgacc
     7741 tccctgtgaa ggtgatccac gtggagagtg gcaagatcct cactggcaca gatgccccaa
     7801 aagccgggca gctggaagcc tggcttgaaa tgaacccagg gtatgaagta gcccccaggt
     7861 cagacagtga agaaagtggc tctgaagagg aggaggagga ggaggaagag gagcagcctc
     7921 agcccgcaca gccccctaca ctgcctgtgg aagaaaagaa gaagattcca gacccagaca
     7981 gcgatgatgt ctctgaggtg gacgcccgac acattattga gaacgccaag caagatgtgg
     8041 acgatgagta cggtgtgtcc caggcccttg ctcgtggcct gcagtcttac tatgctgtgg
     8101 cccatgcagt cacagagaga gtagataagc agtccgccct catggtcaac ggtgtcctca
     8161 aacagtacca gatcaagggt ttggagtggc tggtgtccct gtacaacaac aacctgaatg
     8221 gcatcctggc tgatgagatg gggctgggga ggaccatcca gaccatcgcg ctcatcacat
     8281 acctcatgga gcacaagcgc atcaacgggc ctttcctcat catcgtgcct ctctcgacac
     8341 tgtcaaactg ggcgtatgaa tttgacaagt gggccccctc tgtggtgaag gtttcttaca
     8401 agggctctcc agctgcaagg cgagcttttg tcccacagct tcgcagtggg aagttcaacg
     8461 tcttactgac cacctatgaa tatatcatca aagacaagca tatcctagcc aagatccgct
     8521 ggaagtacat gattgtggat gaaggccacc gcatgaaaaa ccaccactgc aagttgacgc
     8581 aggtccttaa cacacactac gtggcccctc ggcgcctgct tcttacaggc acaccactgc
     8641 agaacaagct accggagctc tgggccctgc ttaacttcct gctccccact atcttcaaga
     8701 gctgcagcac cttcgaacag tggttcaatg caccctttgc catgactgga gaaaaggtgg
     8761 acctgaatga agaggagact atcctcatta ttcgtcgcct acacaaagtt ctgcggccct
     8821 tcctgctgcg gcggctcaag aaggaagttg aagcccagct ccctgagaag gtagagtatg
     8881 tcatcaaatg cgacatgtca gccctgcagc gtgtgctgta ccgtcacatg caggccaaag
     8941 gtgtgctgct gactgacggc tccgagaagg acaagaaggg caaaggtggc accaagacac
     9001 tgatgaacac tattatgcaa ctgcgtaaga tctgcaacca cccctacatg ttccagcaca
     9061 tcgaggagtc cttttctgag cacttggggt tcaccggcgg catcgtgcaa ggattggacc
     9121 tttaccgtgc ctcagggaaa tttgaacttc ttgatagaat tctacccaaa ctccgtgcaa
     9181 cgaaccataa agtgctcctc ttttgccaaa tgacctccct catgaccatc atggaagact
     9241 actttgcata ccgtggcttc aaatacctca ggcttgatgg aaccacaaaa gcagaagacc
     9301 ggggcatgct gttgaaaacc tttaatgaac ctggctctga gtatttcatt ttcctgctca
     9361 gtacccgtgc tggggggctg ggcctgaatc tgcagtcagc tgacactgtg atcatctttg
     9421 acagtgactg gaatccccac caggacctgc aagcacagga tcgagcccat cgcattggac
     9481 agcagaatga ggtgcgtgtt cttcgcctgt gcacggtcaa cagtgtggaa gagaagatac
     9541 tggctgctgc caaatacaaa ctcaatgtgg atcagaaggt gatccaggca ggcatgttcg
     9601 accagaagtc gtccagccat gagaggcgtg ccttcctgca ggccatcctg gagcacgagg
     9661 agcaggatga ggaggaagat gaggtgcctg atgatgagac cgtcaaccag atgattgccc
     9721 ggcacgaaga agagtttgac ctcttcatgc gcatggactt ggaccgccgg cgtgaagaag
     9781 cccgcaaccc caagcggaag ccacgcctga tggaagagga tgagctccca tcctggatca
     9841 tcaaggatga tgccgaggtg gagcggctga catgtgaaga ggaagaggag aagatgttcg
     9901 gccgtggttc tcgccaccgc aaggaggtag actacagcga ctcactgaca gagaagcagt
     9961 ggctcaaggc tatcgaggag ggcacgctgg aggagatcga agaggaggtc cggcagaaga
    10021 aatcttcacg taagcgtaag cgagacagcg aggccggctc ctccaccccg accaccagca
    10081 cccgcagccg tgacaaggat gaggagagca agaagcagaa gaaacgtggg cggccacctg
    10141 ctgagaagct gtccccaaac ccacctaacc tcaccaagaa gatgaagaag atcgtggatg
    10201 ctgtgatcaa gtacaaagac agcagcagtg gacgtcagct cagcgaggtg ttcatccagc
    10261 tcccctctcg caaggagctt cctgagtact atgagctcat ccgaaagcct gtggacttca
    10321 agaagatcaa ggaacgcatc cgaaaccaca agtaccgcag cctcaatgac ctggagaagg
    10381 atgtgatgct gctgtgccag aacgctcaga cgttcaacct cgagggttcc ctgatctatg
    10441 aggactccat cgtcctgcag tctgtcttca ccagcgtacg gcagaagatt gagaaggagg
    10501 acgacagtga aggcgaggaa agcgaggagg aggaggaggg cgaggaggaa ggctccgagt
    10561 ctgagtcccg ctccgtcaag gtgaagatca agctgggccg caaggagaag gcccaggacc
    10621 gactcaaggg gggccgccgg cggccaagcc ggggatcccg ggccaagccg gttgtgagtg
    10681 acgatgacag tgaggaggag caggaggagg accgctcagg aagtggcagt gaggaagacg
    10741 gtggaaccgg tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtaacgcg
    10801 tccggtagtg gaggatccgt cgacgcagat gccggtgaag aataatgtac aagtagcggc
    10861 cgcaaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
    10921 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
    10981 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
    11041 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
    11101 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
    11161 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
    11221 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
    11281 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
    11341 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
    11401 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg ccaccgagta
    11461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
    11521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
    11581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    11641 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
    11701 gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc
    11761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    11821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    11881 gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc
    11941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    12001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tttgtttgaa
    12061 tgaggcttca gtactttaca gaatcgttgc ctgcacgata tcgtacaagt aagattcgac
    12121 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    12181 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    12241 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    12301 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    12361 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    12421 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    12481 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
    12541 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    12601 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    12661 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
    12721 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    12781 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    12841 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    12901 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    12961 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    13021 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    13081 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    13141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13201 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    13261 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    13321 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    13381 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    13441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    13501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    13561 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
    13621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    13681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    13741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    13801 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    13861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    13921 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    13981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    14041 acgtttacaa tttcc
//
