LOCUS       sequence-201981-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces native promoter of zwf_fbp_opcA with Ptrc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::zwf_fbp_opcA
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        212..289
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             290..1372
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(309..328)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1604..1609,1610..1626,1627..1633)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1641..1657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1646..1668
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             2739..3929
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3091..3110)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4568..4759
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4861..5001
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4916..4935
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5016..5033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5187..5775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5267..5286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5946..6737,6738..6806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6569..6588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6807..6911)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6979..6997
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             8777..9568
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatctgggcc gcgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
       61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc
      181 gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc gaatggtgca aaacctttcg
      241 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
      301 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
      361 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
      421 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
      481 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
      541 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
      601 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
      661 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
      721 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
      781 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
      841 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
      901 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
      961 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     1021 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     1081 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     1141 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     1201 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     1261 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1321 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     1381 caattaatgt aagttagcgc gaattgatct ggtttgacag cttatcatcg actgcacggt
     1441 gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt
     1501 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
     1561 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcc
     1621 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaccatg
     1681 gaattcatgg ctcaatccac cacttccgag acccacacca gagatctcga tcgggattgc
     1741 accaccctgt cgcgccatgt tctcgaacag ttgcaaagct ttagccccga agctcaagac
     1801 cttgcggcgt tgatgcagcg cattggcctt gccgctaagc taatcgcccg tcgcctcagc
     1861 cacgcggggc tggtggatga tgccctcggt ttcacggggg aaatcaacgt ccagggcgaa
     1921 gccgtcaaac ggatggacgt ctacgctaac caagttttca tctcagtttt tcggcagagt
     1981 ggcttggtct gtcggctggc ttccgaggaa atggagaagc cctactacat tcctgaaaat
     2041 tgcccgatcg gtcgctatac gctgctctac gaccctttag atggctcggc caacgtcgat
     2101 gtcgatctca acgtcggttc catttttgcg gtgcggcgtc aagagttcta cgacgagtcc
     2161 catgaagcca aggatcttct gcagccaggc gatcgccaaa ttgctgcggg ctatgtcctc
     2221 tatggggcca gcaccttgct ggtctacagc atgggtcagg gcgtccatgt ttttgtactg
     2281 gaccccagct taggtgagtt tgtcctcgcg cagtccgaca tccagctccc gaactcgggt
     2341 cagatctaca gcgtcaatga agggaacttt tggcagtggc ccgagggcta tcgccagtac
     2401 atccgggaaa tgcatcgccg cgaaggctac agcggccgct atagcggagc gttggtagct
     2461 gacttccatc gcattttgat gcagggtggt gtgtttctct atcccgaaac cgtcaagaat
     2521 cccactggca agctgcgact gctctacgaa gcagcaccga tggcctttct ggctgaacaa
     2581 gcaggcggca aggccagtga cggccagaag cctatcctct tgcgccaacc ccaagccctg
     2641 cacgagcggt gcccgctaat tatcggtagc gccgcagatg tggactttaa tgcggtagtt
     2701 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca
     2761 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
     2821 tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc
     2881 tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg
     2941 accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg
     3001 cgatcatggc gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca
     3061 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     3121 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     3181 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     3241 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     3301 agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     3361 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     3421 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     3481 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     3541 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     3601 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     3661 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     3721 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     3781 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     3841 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     3901 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     3961 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     4021 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     4081 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     4141 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     4201 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     4261 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     4321 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     4381 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     4441 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     4501 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     4561 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     4621 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     4681 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     4741 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4801 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4861 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     4921 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     4981 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6241 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6541 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     6601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6961 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
     7021 tgtttgacag cttatcatcg ataggcgaag ccgtgcgatt taatcccaaa ctgccgggct
     7081 cggtcaacaa tttctcgctc catgtcagcc ttgaatcgcc agccgatggc cgtgataatc
     7141 gccggcgaca cagtggttac acggctcgac agtacgactc gttggccctg cttgcccagc
     7201 agtggatgac aacttataca atccccggcg atcgcattac cacccatgca gctgtggata
     7261 gaagtgggtc acgcatggac ccgcgtagtt tccaatgggc gcgtttggct cagcggttgc
     7321 agctattaaa tgagggcacg attccaggct gtggggactc cgctgtcagc gctgctcaat
     7381 tgcctgccag ccccaattga gaggggcgat cgccgagggc cagagcgtca acgttagcct
     7441 gcgaaaagat tggcccagcc tttgttttgc tgaatgttcc cgccattcgt taaatcagtt
     7501 ggtgagattt gagatccctg acagagcgat cgctaatctc tgaactaaat gctcatgctt
     7561 gcttagcttt cccccaagcc ttcacctcac ttgtacagga ggggtatatg acctccgttc
     7621 gtgttcgcat tcatcatcaa aaaggtcaac agccaagcta ccttggccat atttttcaag
     7681 gcgatcgcct gctggcggtt gtcagtggac ggactgaatt agaaacctcg cgtcaagcag
     7741 aagtctggct gaaccagcag ctctccccag ccctgatgta gtcatcccga ttggggctct
     7801 gtgcgatcgc atcctttggc tgtgcctgcg agccgtttca aagcagtctg ggcctggggt
     7861 gcgatcgcat tctgtcgagc ctaggacgat cggatttctc tggctgagct agtcctcaaa
     7921 ccccaaagct ccgcagcatt atcgatcacg gcccttgagt ttatctgaag aaggctgtcg
     7981 attctcgggg atctttctac gctgaagggg aaattcgcgc agccttcgcg gggatgtctt
     8041 cccggatcaa ttcccctgct cgcgcaggct gggtgccaag ctctcgggta acatcaaggc
     8101 ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga aatccagatc
     8161 cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca gaagctgggc
     8221 gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat ccggggtcag
     8281 caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc agggcagatc
     8341 cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac gatgcgtgga
     8401 gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc
     8461 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     8521 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     8581 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     8641 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     8701 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     8761 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     8821 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     8881 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     8941 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     9001 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     9061 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     9121 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     9181 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     9241 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     9301 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     9361 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     9421 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     9481 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     9541 cgagatcacc aaggtagtcg gcaaataatg ctagctcgag cccgcgacat cttcctgctc
     9601 cagaagctcg aaattcgagc tcggtacccg gg
//
