LOCUS       sequence-201982-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the 
            HindIII and XhoI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Braun JE, Friedman LJ, Gelles J, Moore MJ
  TITLE     Synergistic assembly of human pre-spliceosomes across introns and 
            exons.
  JOURNAL   Elife. 2018 Jun 22;7. pii: 37751. doi: 10.7554/eLife.37751.
  PUBMED    29932423
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-06-22"; volume: "7. pii: 37751. doi"; pages: " 
            10.7554/eLife.37751"
COMMENT     Sequence Label: pcDNA5-FRT-TetO-fSNAPc
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..649
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(690..707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    696..808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttaagctgcc accatggaca agctttccag cggtaccgag ctcggatcca gcggacctag
       61 ggaaacctgc ggccgcggct ccggaggctc cggcgggagc ggcatggaca aagactgcga
      121 aatgaagcgc accaccctgg atagccctct gggcaagctg gaactgtctg ggtgcgaaca
      181 gggcctgcac cgtatcatct tcctgggcaa aggaacatct gccgccgacg ccgtggaagt
      241 gcctgcccca gccgccgtgc tgggcggacc agagccactg atgcaggcca ccgcctggct
      301 caacgcctac tttcaccagc ctgaggccat cgaggagttc cctgtgccag ccctgcacca
      361 cccagtgttc cagcaggaga gctttacccg ccaggtgctg tggaaactgc tgaaagtggt
      421 gaagttcgga gaggtcatca gctacagcca cctggccgcc ctggccggca atcccgccgc
      481 caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga tcccctgcca
      541 ccgggtggtg cagggcgacc tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga
      601 gtggctgctg gcccacgagg gccacagact gggcaagcct gggctgggtt agctcgagtc
      661 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      721 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ngtgccactc ccactgtcct
      781 tttcctaata aaatgaggaa attgcatcgc attgtctgag ta
//
