LOCUS       sequence-201984-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc 
            using the KpnI and NotI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Braun JE, Friedman LJ, Gelles J, Moore MJ
  TITLE     Synergistic assembly of human pre-spliceosomes across introns and 
            exons.
  JOURNAL   Elife. 2018 Jun 22;7. pii: 37751. doi: 10.7554/eLife.37751.
  PUBMED    29932423
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-06-22"; volume: "7. pii: 37751. doi"; pages: " 
            10.7554/eLife.37751"
COMMENT     Sequence Label: pcDNA5-FRT-TetO-U1-70K-fSNAPc
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagcgttta acttaagctg ccaccatgga caagctttcc agcggtacca tgacccagtt
       61 cctgccgccc aaccttctgg ccctctttgc cccccgtgac cctattccat acctgccacc
      121 cctggagaaa ctgccacatg aaaaacacca caatcaacct tattgtggca ttgcacctta
      181 tatccgtgaa tttgaggacc ctcgtgatgc ccctcctcca actcgtgctg aaacccgaga
      241 ggagcgcatg gagaggaaaa gacgggaaaa gattgagcgg cgacagcaag aagtggagac
      301 agagcttaaa atgtgggacc ctcacaatga tcccaatgct cagggggatg ccttcaagac
      361 tctcttcgtg gcgagagtga attatgacac aacagaatcc aagctccgta gggaattcga
      421 agtctacgga cctatcaaaa gaatacacat ggtctacagt aagcggtcag gaaagccccg
      481 tggctatgcc ttcatcgagt acgaacacga gcgagatatg cattcagcat ataagcatgc
      541 tgatggcaag aagattgatg gcaggagggt ccttgtggac gtggagaggg gccgaaccgt
      601 gaagggctgg aggccccggc ggctaggagg aggcctcggt ggtacaagaa gaggaggggc
      661 tgatgtgaac atccggcatt caggccgcga tgacacctcc cgctacgatg agaggcccgg
      721 cccctccccg cttccgcaca gggaccggga ccgggaccgt gagcgggagc gcagagagcg
      781 gagccgggag cgagacaagg agcgagaacg gcgacgctcc cgctcccggg accggcggan
      841 gcgctcacgg agtcgcgaca aggaggagcg gaggcgctcc agggagcgg
//
