LOCUS       sequence-201986-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc 
            using the KpnI and NotI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Braun JE, Friedman LJ, Gelles J, Moore MJ
  TITLE     Synergistic assembly of human pre-spliceosomes across introns and 
            exons.
  JOURNAL   Elife. 2018 Jun 22;7. pii: 37751. doi: 10.7554/eLife.37751.
  PUBMED    29932423
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-06-22"; volume: "7. pii: 37751. doi"; pages: " 
            10.7554/eLife.37751"
COMMENT     Sequence Label: pcDNA5-FRT-TetO-U2B''-fSNAPc
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagcgttta acttaagctg ccaccatgga caagctttcc agcggtacca tggatatcag
       61 accaaatcat acaatttata tcaacaatat gaatgacaaa attaaaaagg aggagctcaa
      121 acggtcttta tatgccctgt tttctcagtt tggtcatgtg gtggacattg tggctttaaa
      181 gaccatgaag atgagggggc aggcctttgt catatttaag gaactgggct catccacaaa
      241 tgccttgagg caacttcagg gcttcccatt ttatggtaaa ccaatgcgaa tacagtatgc
      301 aaaaacagat tcggatataa tatcaaaaat gcgtggaact tttgctgaca aagaaaagaa
      361 aaaagaaaag aaaaaagcca aaactgtgga acagactgca acaaccacaa acaaaaagcc
      421 tggccaggga actccaaatt cagctaatac acagggcaac agtaccccta atcctcaggt
      481 ccctgattac cctccaaact atattttatt ccttaataac ttaccagaag aaaccaacga
      541 aatgatgtta tccatgctgt ttaatcagtt ccctggcttc aaggaagtac gtctggttcc
      601 agggaggcat gacattgctt ttgttgaatt tgaaaatgat gggcaggctg gagctgccag
      661 ggatgcttta cagggattta agatcacacc gtcccacgcc atgaaaatta catacgccaa
      721 gaaatgcggc cgcggctccg gangctccgg cgggagcggc atggacaaag actgcgaaat
      781 gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg
      841 cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgangccc gtggnagtgc
      901 ctgccccagc cgccgtgctg ggcggaccag agcactga
//
