LOCUS       sequence-202005-        6558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani 
            P, Raczkowski A, Petrey DS, Pon LA, Polleux F
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in 
            mammalian neurons.
  JOURNAL   Science. 2017 Nov 3;358(6363):623-630. doi: 10.1126/science.aan6009.
  PUBMED    29097544
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "623-630"
COMMENT     Sequence Label: pCAG-PDZD8HA
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3453..3479
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3573..3592)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3638..3693
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3692..3711)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4058..4074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4058..4074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4071..4093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4082..4098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4106..4112,4113..4130,4131..4136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4151..4172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4231..4426
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4277..4412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4432..4566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4469..4488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4523..4542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4634..4651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4805..5393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4885..4904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5564..6355,6356..6424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6187..6206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6425..6529)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattgctcga gatggggctg ctgctcctga tcctggcgtc ggccgtgctg ggctcgttcc
       61 tcacgctgct cgcacagttc ctgctactct accgcagaca gcccgagccg cgggcggacg
      121 aagcggcccg cgcgggcgac ggcttccgct accttaagcc ggtgccgggc ctgcccctca
      181 gggagtacct ttatggcggc ggcgccgagg agctcgctgc ttgctcctcc gaggccggcg
      241 ccagctcgac cccgaccccc gacagcccgg ccccgccgac gctggagacc tgctacttcc
      301 tcaacgccac catcctgttc ctgttccggg agctgcggga caccgcgctc gcccgccgct
      361 gggtcaccaa gaagatcaag gtggagttcg aggagctgct gcagaccaag acggccggcc
      421 gcctgctgga ggggctgagt ctgcgcgacg tgttcctggg cgacacggtg cccttcatca
      481 agaccatccg gctggtgcgg cccgtggtgg cctcgggcac cggcgagccc gacgaccccg
      541 acggggacgc gctgcccgcc acctgcccgg aggagctggc ctttgaagcg gaggtggagt
      601 acaacggcgg tttccacctg gccatcgacg tggacctggt gtttggcaag tccgcctacc
      661 tgttcgtgaa gctgtctcgc gtggtgggga ggctgcgctt cgtcctcacc cgcgtgccct
      721 tcacccactg gttcttctcc ttcgtggagg acccgctgat tgacttcgag gtgcgctccc
      781 agttcgaggg gcggcccatg ccccagctca cctccatcat cgtcaaccag ctcaagaaga
      841 tcatcaagcg caagcacacc ctgcccagtt acaagatcag gtttaagccg ttttttccat
      901 accaagcctt gcaaggattt gaagaagatg aagaacttat ccatattcaa caatgggcac
      961 ttactgaagg ccggcttaaa gttacattgt tagaatgtag caggttattc atttttggat
     1021 cctatgacag agaaacaaat gttcattgca cacttgagct gagcagtggt gtttgggaag
     1081 aaaaacaaag gagttctatt aagacggttg aattaataaa agggaattta caaagtgtcg
     1141 gacttacact tcgtcttgtt cagtcaactg atgggtatgc tgggcatgtc ataatcgaaa
     1201 ctgtggcccc aaactcacct gctgcgatgg cagatcttca acggggagat cggctcattg
     1261 ctattggagg tgtgaaaatc acatcgaccc tgcaggtgct gaagctcatc aagcaggctg
     1321 gggaccgcgt gctggtgtac taccagaggc cggctgggca gagcagccaa gacagcctgg
     1381 gccagttgga agaaagcttc ttgtccagct cctgccaggc agcctacgaa gaggacgctg
     1441 ctgggctgtc agcggacact gaaaacagag acctagattc tgaattcgaa gacttggcga
     1501 gtgatgtcag agtacagaca gagttgaaag aggagacaca gccgttgagt cacagtccca
     1561 aacgtactcc aacaacactt tctattaaac cccttggcgc tatatccccg gttttaaatc
     1621 gtaaattaat ttcaggaatc cacccaccac cacaaaaact tccatccaaa gaaggaaata
     1681 aaccatcaac tctaaaaact tcagagacca cagaagcagc acaagtgtcc aaaccccaag
     1741 gacccacttt caaaccacct gtgccaccgc gaccacaggg gagagtgcct ctgcctccca
     1801 ctgacacctc agcccaagca gacccggaag ccccagaaaa gccagacaag gtgctgctcc
     1861 ctcctcctcc tgcagataaa cctgccgaga agcaagtgaa gagtgtggat caaggagaag
     1921 atgtggcagc aggtaagcag tcttcagcga agcaggaagg agtgaaagat cttccttcag
     1981 aaagttctgc tcctactaaa gactcttcag atgaccctca gatgtgggaa tcctcagagg
     2041 ttctatatcg caacaaggtg ggaaagtggt caagaaccag agcgtcctgt gtgtttgaca
     2101 tagaagcctg ccataggtac ctgaacatcg cactgtggtg cagggatcct ttcaagttag
     2161 gaggtctcat ctgtttgggg catgttagtt taaaacttga ggaagtggct ttaggatgtc
     2221 tagctacatc aaacatggaa taccttacaa agttcagact ggaaccccct acacctaagg
     2281 ccatggtcac tagaactgca ctacgtaatc tgagtatgca aaagggcttt aatgacaaat
     2341 tttgttttgg tgacattact attcatttca agtatttgaa ggaaggagaa ccagaccacc
     2401 acatagttcc taatgtagag aaagaaaaag aactccattt ggttgaggag gtttctacac
     2461 tccctaaaga agagcatttt gtgggacaga tgagtttgtc agaaaataaa catagtttcc
     2521 aagatactca gttccagaac ccaacttggt gtgattactg taagaaaaaa gtttggacaa
     2581 aagctgcttc ccagtgtatg ttctgtgctt atgtttgtca taaaaaatgt caggaaaagt
     2641 gtctagctga gacacctctt tgtggagcaa ctgagaggcg aatagaccgg accctgaaaa
     2701 acctcaggct ggaaggacag gacccactct tgggccttcc tcctcgtgtt gagatagaag
     2761 ctaacaagtc agtcaataaa acaacagggt tgacaaggca catcattaat acgagctctc
     2821 gtttactaaa tctgcgtcaa gtctctaaaa ctcgcctttc tgaaccagga acagatcttg
     2881 tagaaccttc accaaaacac acacccaata cgtcagacaa tgaaggcagt gacacagagg
     2941 tctgtggttc aaacagtcct tccaaacgag gaaacagtgc aggaataaag ttaatgagaa
     3001 aggaaggagg gctggatgac agtgttttca ttgcagtaaa ggaaattggt cgtgatctgt
     3061 acagaggctt gccgacagaa gagaggatcc agaagctgga gttcatgttg gataagctac
     3121 agaatgaaat tgatcaggag ctggaacaca acaattccct tgttagagaa gaaaaggaaa
     3181 caaatgatac aaggaaaaag tcagttcttt ctgctgcttt agctaaatcg ggagaaagac
     3241 tacaagctct gacacttctg atgatccact acagagcggg aatcgaagat attgaaaccc
     3301 tagaaaatct gtctttagac cagcactcga aaaagatgaa caagtacgca gatgatacag
     3361 aagaagacct cgatagtgag ataagccagt tgatagactc tcagccattc agcaacattt
     3421 ccgatgactt gttcggccca tctgagtcag tgtacccata cgatgttcca gattacgctt
     3481 aagcggccgc aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     3541 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     3601 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     3661 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     3721 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     3781 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     3841 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     3901 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     3961 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     4021 ctcgacctgc accgtcgacc agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4081 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4141 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4201 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     4261 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4321 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4381 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     4441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4561 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5221 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6541 gaaaagtgcc acctgggt
//
