LOCUS       sequence-202007-        7041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of PDZD8, sh resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani 
            P, Raczkowski A, Petrey DS, Pon LA, Polleux F
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in 
            mammalian neurons.
  JOURNAL   Science. 2017 Nov 3;358(6363):623-630. doi: 10.1126/science.aan6009.
  PUBMED    29097544
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "623-630"
COMMENT     Sequence Label: pCAG-PDZD8HA_sh_resistant
FEATURES             Location/Qualifiers
     source          1..7041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     343..365
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     427..446
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3921..3947
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4041..4060)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4106..4161
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4160..4179)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4526..4542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4526..4542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4539..4561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4550..4566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4574..4580,4581..4598,4599..4604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4619..4640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4699..4894
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4745..4880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4900..5034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4937..4956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4991..5010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5102..5119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5273..5861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5353..5372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6032..6823,6824..6892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6655..6674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6893..6997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
       61 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
      121 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
      181 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
      241 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
      301 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
      361 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
      421 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgaga
      481 tggggctgct gctcctgatc ctggcgtcgg ccgtgctggg ctcgttcctc acgctgctcg
      541 cacagttcct gctactctac cgcagacagc ccgagccgcg ggcggacgaa gcggcccgcg
      601 cgggcgacgg cttccgctac cttaagccgg tgccgggcct gcccctcagg gagtaccttt
      661 atggcggcgg cgccgaggag ctcgctgctt gctcctccga ggccggcgcc agctcgaccc
      721 cgacccccga cagcccggcc ccgccgacgc tggagacctg ctacttcctc aacgccacca
      781 tcctgttcct gttccgggag ctgcgggaca ccgcgctcgc ccgccgctgg gtcaccaaga
      841 agatcaaggt ggagttcgag gagctgctgc agaccaagac ggccggccgc ctgctggagg
      901 ggctgagtct gcgcgacgtg ttcctgggcg acacggtgcc cttcatcaag accatccggc
      961 tggtgcggcc cgtggtggcc tcgggcaccg gcgagcccga cgaccccgac ggggacgcgc
     1021 tgcccgccac ctgcccggag gagctggcct ttgaagcgga ggtggagtac aacggcggtt
     1081 tccacctggc catcgacgtg gacctggtgt ttggcaagtc cgcctacctg ttcgtgaagc
     1141 tgtctcgcgt ggtggggagg ctgcgcttcg tcctcacccg cgtgcccttc acccactggt
     1201 tcttctcctt cgtggaggac ccgctgattg acttcgaggt gcgctcccag ttcgaggggc
     1261 ggcccatgcc ccagctcacc tccatcatcg tcaaccagct caagaagatc atcaagcgca
     1321 agcacaccct gcccagttac aagatcaggt ttaagccgtt ttttccatac caagccttgc
     1381 aaggatttga agaagatgaa gaacttatcc atattcaaca atgggcactt actgaaggcc
     1441 ggcttaaagt tacattgtta gaatgtagca ggttattcat ttttggatcc tatgacagag
     1501 aaacaaatgt tcattgcaca cttgagctga gcagtggtgt ttgggaagaa aaacaaagga
     1561 gttctattaa gacggttgaa ttaataaaag ggaatttaca aagtgtcgga cttacacttc
     1621 gtcttgttca gtcaactgat gggtatgctg ggcatgtcat aatcgaaact gtggccccaa
     1681 actcacctgc tgcgatggca gatcttcaac ggggagatcg gctcattgct attggaggtg
     1741 tgaaaatcac atcgaccctg caggtgctga agctcatcaa gcaggctggg gaccgcgtgc
     1801 tggtgtacta ccagaggccg gctgggcaga gcagccaaga cagcctgggc cagttggaag
     1861 aaagcttctt gtccagctcc tgccaggcag cctacgaaga ggacgctgct gggctgtcag
     1921 cggacactga aaacagagac ctagattctg aattcgaaga cttggcgagt gatgtcagag
     1981 tacagacaga gttgaaagag gagacacagc cgttgagtca cagtcccaaa cgtactccaa
     2041 caacactttc tattaaaccc cttggcgcta tatccccggt tttaaatcgt aaattaattt
     2101 caggaatcca cccaccacca caaaaacttc catccaaaga aggaaataaa ccatcaactc
     2161 taaaaacttc agagaccaca gaagcagcac aagtgtccaa accccaagga cccactttca
     2221 aaccacctgt gccaccgcga ccacagggga gagtgcctct gcctcccact gacacctcag
     2281 cccaagcaga cccggaagcc ccagaaaagc cagacaaggt gctgctccct cctcctcctg
     2341 cagataaacc tgccgagaag caagtgaaga gtgtggatca aggagaagat gtggcagcag
     2401 gtaagcagtc ttcagcgaag caggaaggag tgaaagatct tccttcagaa agttctgctc
     2461 ctactaaaga ctcttcagat gaccctcaga tgtgggaatc ctcagaggtt ctatatcgca
     2521 acaaggtggg aaagtggtca agaaccagag cgtcctgtgt gtttgacata gaagcctgcc
     2581 ataggtacct gaacatcgca ctgtggtgca gggatccttt caagttagga ggtctcatct
     2641 gtttggggca tgttagttta aaacttgagg aagtggcttt aggatgtcta gctacatcaa
     2701 acatggaata ccttacaaag ttcagactgg aaccccctac acctaaggcc atggtcacta
     2761 gaactgcact acgtaatctg agtatgcaaa agggctttaa tgacaaattt tgttttggtg
     2821 acattactat tcatttcaag tatttgaagg aaggagaacc agaccaccac atagttccta
     2881 atgtagagaa agaaaaagaa ctccatttgg ttgaggaggt ttctacactc cctaaagaag
     2941 agcattttgt gggacagatg agtttgtcag aaaataaaca tagtttccaa gatactcagt
     3001 tccagaaccc aacttggtgt gattactgta agaaaaaagt ttggacaaaa gctgcttccc
     3061 agtgtatgtt ctgtgcttat gtttgtcata aaaaatgtca ggaaaagtgt ctagctgaga
     3121 cacctctttg tggagcaact gagcgacgga tagaccggac cctgaaaaac ctcaggctgg
     3181 aaggacagga cccactcttg ggccttcctc ctcgtgttga gatagaagct aacaagtcag
     3241 tcaataaaac aacagggttg acaaggcaca tcattaatac gagctctcgt ttactaaatc
     3301 tgcgtcaagt ctctaaaact cgcctttctg aaccaggaac agatcttgta gaaccttcac
     3361 caaaacacac acccaatacg tcagacaatg aaggcagtga cacagaggtc tgtggttcaa
     3421 acagtccttc caaacgagga aacagtgcag gaataaagtt aatgagaaag gaaggagggc
     3481 tggatgacag tgttttcatt gcagtaaagg aaattggtcg tgatctgtac agaggcttgc
     3541 cgacagaaga gaggatccag aagctggagt tcatgttgga taagctacag aatgaaattg
     3601 atcaggagct ggaacacaac aattcccttg ttagagaaga aaaggaaaca aatgatacaa
     3661 ggaaaaagtc agttctttct gctgctttag ctaaatcggg agaaagacta caagctctga
     3721 cacttctgat gatccactac agagcgggaa tcgaagatat tgaaacccta gaaaatctgt
     3781 ctttagacca gcactcgaaa aagatgaaca agtacgcaga tgatacagaa gaagacctcg
     3841 atagtgagat aagccagttg atagactctc agccattcag caacatttcc gatgacttgt
     3901 tcggcccatc tgagtcagtg tacccatacg atgttccaga ttacgcttaa gcggccgcaa
     3961 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     4021 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     4081 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     4141 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     4201 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     4261 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     4321 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     4381 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     4441 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcac
     4501 cgtcgaccag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4561 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4621 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4681 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4801 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4861 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     4921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     5041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7021 ctgggtcgac attgattatt g
//
