LOCUS       sequence-202010-       11463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING V155M delta 368 and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP
FEATURES             Location/Qualifiers
     source          1..11463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5867..6047
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7575..7594)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7808..7827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7927..7949
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7987..8005)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8073..8177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8178..8246,8247..9038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8396..8415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9209..9797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9698..9717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9951..9968
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10038..10058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10043..10372
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10223..10358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catgccccac
     4501 tccagcctgc atccatccat cccgtgtccc aggggtcacg gggcccagaa ggcagccttg
     4561 gttctgctga gtgcctgcct ggtgaccctt tgggggctag gagagccacc agagcacact
     4621 ctccggtacc tggtcctcca cctagcctcc ctgcagctgg gactgctgtt aaacggggtc
     4681 tgcagcctgg ctgaggagct gcgccacatc cactccaggt accggggcag ctactggagg
     4741 actgtgcggg cctgcctggg ctgccccctc cgccgtgggg ccctgttgct gctgtccatc
     4801 tatttctact actccctccc aaatgcggtc ggcccgccct tcacttggat gcttgccctc
     4861 ctgggcctct cgcaggcact gaacatcctc ctgggcctca agggcctggc cccagctgag
     4921 atctctgcag tgtgtgaaaa agggaatttc aacatggccc atgggctggc atggtcatat
     4981 tacatcggat atctgcggct gatcctgcca gagctccagg cccggattcg aacttacaat
     5041 cagcattaca acaacctgct acggggtgca gtgagccagc ggctgtatat tctcctccca
     5101 ttggactgtg gggtgcctga taacctgagt atggctgacc ccaacattcg cttcctggat
     5161 aaactgcccc agcagaccgg tgaccgtgct ggcatcaagg atcgggttta cagcaacagc
     5221 atctatgagc ttctggagaa cgggcagcgg gcgggcacct gtgtcctgga gtacgccacc
     5281 cccttgcaga ctttgtttgc catgtcacaa tacagtcaag ctggctttag ccgggaggat
     5341 aggcttgagc aggccaaact cttctgccgg acacttgagg acatcctggc agatgcccct
     5401 gagtctcaga acaactgccg cctcattgcc taccaggaac ctgcagatga cagcagcttc
     5461 tcgctgtccc aggaggttct ccggcacctg cggcaggagg aaaaggaaga ggttactgtg
     5521 ggcagcttga agacctcagc ggtgcccagt acctccacga tgtcccaaga gcctgagctc
     5581 ctcatcagtg gaatgtgact cgagacctag aaaaacatgg agcaatcaca agtagcaata
     5641 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5701 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     5761 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa
     5821 atcgtcgaga gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt
     5881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     6121 ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg
     6181 cagaagtact tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc
     6241 ccagatccta aggattacat taagtttact aacatttata taatgattta tagtttaaag
     6301 tataaactta tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga
     6361 gatgattact attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt
     6421 aattttccca caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc
     6481 catttactcc tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag
     6541 taacaaatta cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca
     6601 aacctgaata gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca
     6661 agttttaaca agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt
     6721 ttctggcaac aagactatac aatataacct taaatatgac ttcaaataat tgttggaact
     6781 tgataaaact aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta
     6841 aaaagggaac atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt
     6901 gtcaaaacaa gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag
     6961 attttgtgtt ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca
     7021 tgtaaatagt tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt
     7081 atcttggaag aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct
     7141 cacacctttg ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt
     7201 cttctctgag atcatttcac attgcagata gaaaacctga gactggggta atttttatta
     7261 aaatctaatt taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata
     7321 ttatcatata aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg
     7381 tggatcaaaa atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat
     7441 cagaaaaaca gcacagtata acaactttat ttagcattta caatctatta ggtattataa
     7501 gtaatctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     7561 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     7621 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     7681 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     7741 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     7801 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     7861 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7921 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7981 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8041 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8101 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8161 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8221 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8281 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8341 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8401 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8461 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8521 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8581 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8641 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8701 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8761 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8821 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8881 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8941 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9001 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9901 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9961 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10021 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    10081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    10141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    10201 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    10321 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
    10381 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
    10441 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    10501 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    10561 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    10621 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    10681 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    10741 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    10801 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    10861 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10921 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10981 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    11041 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    11101 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    11161 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    11221 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    11281 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    11341 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    11401 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    11461 gtg
//
