LOCUS       sequence-202019-        8025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human OCT4 genomic targeting donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: OCT4-2A-eGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..8025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..33
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    150..171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(186..191,192..209,210..216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    224..240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     248..264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     248..264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1051..1107
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1108..1110,1111..1113,1114..1827)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1108..1110,1111..1113,1114..1824)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1153..1174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1414..1433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1761..1782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1858..1891
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1987..2010)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2394..2413
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2501..3100
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2501..2520)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    3657..3690
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4501..4520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4502..4520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4527..4544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4527..4543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4536..4558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4684..5112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4771..4790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4981..5002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5456..6250
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5510..5529)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6120..6139
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6268..6336,6337..7128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6486..6505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7299..7887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7788..7807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
       61 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      241 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggtaccga gctcggatcc
      301 actagtaacg gccgccagtg tgctggaatt cgcccttttc ctgcaggtcc cacctgcaca
      361 gatatgcaaa gcagaaaccc tcgtgcaggc ccgaaagaga aagcgaacca gtatcgagaa
      421 ccgagtgaga ggcaacctgg agaatttgtt cctgcagtgc ccgaaaccca cactgcagca
      481 gatcagccac atcgcccagc agcttgggct cgagaaggat gtgagtgcca tgtctctctg
      541 cgggctccat ctctttcccc tgtcaccacc tcgctttccc tagctctggc tcctccaact
      601 gctctagggc tgttggcttt ggacagaatg tccaagcagt caggcctgtc tcagctcatt
      661 ctctaatgtc ctcctctaac tgctctaggg ctgttggctt tggatagaat gtccaagcag
      721 agtcaggccc gtctcagctc attgtctaat gtcattctcc tttctgtcat tcacttgcag
      781 gtggtccgag tgtggttctg taaccggcgc cagaagggca agcgatcaag cagcgactat
      841 gcacaacgag aggattttga ggctgctggg tctcctttct cagggggacc agtgtccttt
      901 cctctggccc cagggcccca ttttggtacc ccaggctatg ggagccctca cttcactgca
      961 ctgtactcct cggtcccttt ccctgagggg gaagcctttc cccctgtctc cgtcaccact
     1021 ctgggctctc ccatgcattc aaacgctagc gccactaact tctccctgtt gaaacaagca
     1081 ggggatgtcg aagagaatcc cgggccaatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1321 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1561 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1801 ctcggcatgg acgagctgta caagtaagaa ttccgatcat attcaataac ccttaatata
     1861 acttcgtata atgtatgcta tacgaagtta ttaggtctga agaggagttt acgtccagcc
     1921 aagcttagga tctcgacctc gaaattctac cgggtagggg aggcgctttt cccaaggcag
     1981 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     2041 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     2101 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     2161 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     2221 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     2281 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggagcagc ggggcgggct
     2341 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     2401 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     2461 gacctgcatc catctagatc tcgagcagct gaagcttacc atgaccgagt acaagcccac
     2521 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     2581 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     2641 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     2701 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     2761 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     2821 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     2881 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     2941 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccacaa
     3001 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3061 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     3121 gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc
     3181 agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca
     3241 tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta
     3301 cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact
     3361 attgctttat gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt
     3421 aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca
     3481 ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag
     3541 tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta
     3601 ttgttttgcc aagttctaat tccatcagaa gctggtcgag atccggaacc cttaatataa
     3661 cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc actaggcgcg
     3721 ccggtgcctg cccttctagg aatgggggac agggggaggg gaggagctag ggaaagaaaa
     3781 cctggagttt gtgccagggt ttttgggatt aagttcttca ttcactaagg aaggaattgg
     3841 gaacacaaag ggtgggggca ggggagtttg gggcaactgg ttggagggaa ggtgaagttc
     3901 aatgatgctc ttgattttaa tcccacatca tgtatcactt ttttcttaaa taaagaagcc
     3961 tgggacacag tagatagaca cacttatctt ggtttgtcct tcagttactg aggtggggat
     4021 gggaatatcc aatgctcata cccaagtgac cctgaaacta aggtgccatt tacactcctt
     4081 aaggtcacac aacatcagag ggagagctgg gattgcagcc aagtttattt gtacagggcc
     4141 ctgtgatagg ctagttccca aaagcctgtg atgcaagaac ttttgcccat agactcagtc
     4201 accatgtagc tgttacctgt tcagagctgg ctttttgctt tcccacccta ctctggaatt
     4261 cttaaatggc tttatactta gaaatcatct tatttctgtt gaacctagat caccccaacc
     4321 agaaacttct attaatactt tgtgctttct tgataccagg gtctatttgg tttccactta
     4381 aggtttttgc atactctgcc cataagtgac tcattagtta cggccggcca aaagggcgaa
     4441 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg
     4501 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4561 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4621 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4681 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4741 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4801 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4861 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4921 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4981 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5041 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5101 ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta
     5161 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     5221 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     5281 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     5341 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     5401 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     5461 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5521 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5581 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     5641 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5701 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5761 cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5821 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5881 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5941 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6001 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6061 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6121 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6181 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6241 gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7441 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7921 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7981 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagc
//
