LOCUS       sequence-202026-        5168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mitochondrial calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F
  TITLE     LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance 
            and Neurotransmitter Release Properties at Excitatory Synapses along
            Cortical Axons.
  JOURNAL   PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul.
  PUBMED    27429220
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2016-07-18"; volume: "14(7):e1002516. doi"; pages: " 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul"
COMMENT     Sequence Label: pCAG mito-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..5168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     464..486
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     548..567
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             601..675
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(703..711,712..729,730..732,733..765,766..768,769..792,
                     793..819,820..876,877..882,883..1152,1153..1170,1171..1605,
                     1606..1611,1612..2055)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(703..711,712..729,730..732,733..765,766..768,769..792,
                     793..819,820..876,877..882,883..1152,1153..1170,1171..1605,
                     1606..1611,1612..2055)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      799..808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1089..1110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1216..1237)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1477..1496)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2146..2165)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2211..2266
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2265..2284)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2657..2673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2657..2673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2670..2692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2681..2697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2705..2711,2712..2729,2730..2735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2750..2771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2830..3025
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2876..3011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3031..3165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3068..3087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3122..3141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3233..3250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3404..3992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3484..3503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4163..4954,4955..5023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4786..4805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5024..5128)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
       61 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
      121 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
      181 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
      241 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
      301 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
      361 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
      421 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
      481 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
      541 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgctcgag
      601 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
      661 gtgccgcgcg ccaagatcca ttcgttgggg gatccaccgg tcatgggttc tcatcatcat
      721 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      781 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
      841 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
      901 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
      961 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1021 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
     1081 cgcgatcaca tggtcctgct ggagttcgtg actgccgccg ggatcactct cggcatggac
     1141 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
     1201 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1261 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1321 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1381 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1441 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1501 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1561 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
     1621 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
     1681 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
     1741 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     1801 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acacagacag tgaagaagaa
     1861 attagagaag cgttccgtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     1921 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     1981 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     2041 atgacagcga agtgagcggc cgcaattcac tcctcaggtg caggctgcct atcagaaggt
     2101 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     2161 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     2221 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     2281 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     2341 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     2401 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     2461 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     2521 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     2581 cttatggaga tccctcgacc tgcaccgtcg accagctggt cgacggtgca ccgtcgacca
     2641 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2701 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2761 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2821 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2881 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2941 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3001 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     3061 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3121 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     3181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4141 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4201 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4261 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4321 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4381 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4441 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4501 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4561 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4621 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4681 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4741 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4801 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4861 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4921 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4981 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5041 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5101 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     5161 cattgatt
//
