LOCUS       sequence-202032-        5519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Excitatory presynaptic marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F
  TITLE     LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance 
            and Neurotransmitter Release Properties at Excitatory Synapses along
            Cortical Axons.
  JOURNAL   PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul.
  PUBMED    27429220
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2016-07-18"; volume: "14(7):e1002516. doi"; pages: " 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul"
COMMENT     Sequence Label: pCAG vGlut1 mCherry
FEATURES             Location/Qualifiers
     source          1..5519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1296..1307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1706..2416
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1851..1869)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2119..2138
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2507..2526)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2572..2627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2626..2645)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3018..3034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3018..3034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3031..3053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3042..3058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3066..3072,3073..3090,3091..3096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3111..3132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3191..3386
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3237..3372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3392..3526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3429..3448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3483..3502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3594..3611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3765..4353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3845..3864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4524..5315,5316..5384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5147..5166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5385..5489)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaggccacc atggagttcc ggcaggagga gtttcggaag ctggcggggc gcgccctggg
       61 gaggctgcac cggttactgg agaagcggca ggaaggcgcg gagacattgg agctgagcgc
      121 cgacgggcgc ccagtgacca cacacacgcg ggacccgccg gtggtggact gcacttgctt
      181 tggcctccct cgccgctaca tcatcgcgat catgagcggt ctgggtttct gcatcagctt
      241 tggcatccgc tgcaacctgg gcgtggccat cgtatccatg gtcaacaaca gtacaaccca
      301 ccgtgggggc cacgtggtgg tgcagaaagc ccagttcaac tgggatccag agactgtcgg
      361 cctcatacat ggctcctttt tctgggggta cattgtcact cagattcctg gaggatttat
      421 ctgccaaaaa ttcgcagcca acagggtctt tggctttgcc attgtggcta cctccaccct
      481 aaatatgttg atcccttcag cagcccgtgt tcactatggc tgtgtcatct tcgtgaggat
      541 ccttcaggga ttggtggagg gggtcacata ccctgcttgc catggcatct ggagcaaatg
      601 ggcccctccc ttagaacgga gtcggctggc gacgacagcc ttttgcggtt cctatgccgg
      661 ggcagtggtt gccatgcctc tggctggggt cctggtacag tattcaggat ggagttctgt
      721 cttctatgtc tatggcagct tcgggatctt ttggtacctg ttctggttgc ttgtctccta
      781 cgagtcacct gcactacacc ccagcatctc cgaggaggag cgcaaataca ttgaggatgc
      841 catcggagaa agcgccaagc tcatgaaccc tgttacgaag tttaacacac cctggaggcg
      901 cttctttacc tccatgccgg tctatgccat cattgtcgcc aacttttgcc gcagctggac
      961 tttctacctg ctcctcatct cccagcccgc ctactttgaa gaagtgttcg gctttgagat
     1021 cagcaaggtg ggactggtgt cggcactgcc tcaccttgtc atgactatca tcgtacccat
     1081 cggaggccag atcgccgact tcctgcgcag tcgtcatata atgtccacga ccaatgtgcg
     1141 aaagctgatg aactgcgggg gtttcgggat ggaagctacg ctgctgctgg tggtcggata
     1201 ctcacactcc aagggcgtgg ccatctcctt cctggtcctg gctgtgggct tcagtggctt
     1261 tgctatctct gggtttaacg tgaaccactt ggacatcgcc cctcgatatg ccagcatctt
     1321 gatgggcatt tccaatggcg tgggcacact gtctgggatg gtgtgcccca tcatcgtggg
     1381 tgcaatgacc aagcacaaga cgcgggagga gtggcagtac gtgttcctca tagcctccct
     1441 ggtgcactat ggaggtgtca tcttctatgg ggtctttgct tcgggagaga aacagccgtg
     1501 ggcagagccg gaggagatga gcgaggagaa gtgtggcttt gttggccacg accagctggc
     1561 tggcagtgac gaaagtgaaa tggaagacga ggttgagccc ccgggggcac cccccgcacc
     1621 tccgccttcc tacggggcca cacacagcac agttcagcct ccaaggcccc caccccctgt
     1681 ccgggactac gaattcggtg gttctatggt gagcaagggc gaggaggata acatggccat
     1741 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     1801 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1861 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1921 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1981 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2041 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2101 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2161 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2221 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     2281 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     2341 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     2401 cgagctgtac aagtaggcgg ccgcaattca ctcctcaggt gcaggctgcc tatcagaagg
     2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2701 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     2761 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     2821 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     2881 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     2941 tcttatggag atccctcgac ctgcaccgtc gaccagctgg tcgacggtgc accgtcgacc
     3001 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3121 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3181 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3241 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3301 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3361 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     3421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3481 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     3541 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3601 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3661 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3721 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3781 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3841 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3901 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3961 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4021 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4081 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4141 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4201 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4261 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4321 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4381 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4441 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4501 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4561 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4621 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4681 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4741 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4801 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4861 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4921 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4981 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5041 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5101 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5161 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5221 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5281 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5341 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5401 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5461 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtc
//
