LOCUS       sequence-202033-        5831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA
  TITLE     Primary structure of ICAM-1 demonstrates interaction between members
            of the immunoglobulin and integrin supergene families.
  JOURNAL   Cell 1988 Mar 25;52(6):925-33.
  PUBMED    3349522
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1988-03-25"; volume: "52(6)"; pages: "925-33"
COMMENT     Sequence Label: Human ICAM-1
FEATURES             Location/Qualifiers
     source          1..5831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        124..503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     658..678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     752..771
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        752..770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2792..2857
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2987..3007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3279..3413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3316..3335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3370..3389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4260..4395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4890..4909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(5022..5480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5159..5180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5374..5393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            complement(join(5603..5622,5623..5707,5708..5782))
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
ORIGIN
        1 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
       61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      121 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      361 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      421 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
      721 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
      781 cttctagaga tccctcgacc tcgagatcca ttgtgctcta aagtcgacgc tgagctcctc
      841 tgctactcag agttgcaacc tcagcctcgc tatggctccc agcagccccc ggcccgcgct
      901 gcccgcactc ctggtcctgc tcggggctct gttcccagga cctggcaatg cccagacatc
      961 tgtgtccccc tcaaaagtca tcctgccccg gggaggctcc gtgctggtga catgcagcac
     1021 ctcctgtgac cagcccaagt tgttgggcat agagaccccg ttgcctaaaa aggagttgct
     1081 cctgcctggg aacaaccgga aggtgtatga actgagcaat gtgcaagaag atagccaacc
     1141 aatgtgctat tcaaactgcc ctgatgggca gtcaacagct aaaaccttcc tcaccgtgta
     1201 ctggactcca gaacgggtgg aactggcacc cctcccctct tggcagccag tgggcaagaa
     1261 ccttacccta cgctgccagg tggagggtgg ggcaccccgg gccaacctca ccgtggtgct
     1321 gctccgtggg gagaaggagc tgaaacggga gccagctgtg ggggagcccg ctgaggtcac
     1381 gaccacggtg ctggtgagga gagatcacca tggagccaat ttctcgtgcc gcactgaact
     1441 ggacctgcgg ccccaagggc tggagctgtt tgagaacacc tcggccccct accagctcca
     1501 gacctttgtc ctgccagcga ctcccccaca acttgtcagc ccccgggtcc tagaggtgga
     1561 cacgcagggg accgtggtct gttccctgga cgggctgttc ccagtctcgg aggcccaggt
     1621 ccacctggca ctgggggacc agaggttgaa ccccacagtc acctatggca acgactcctt
     1681 ctcggccaag gcctcagtca gtgtgaccgc agaggacgag ggcacccagc ggctgacgtg
     1741 tgcagtaata ctggggaacc agagccagga gacactgcag acagtgacca tctacagctt
     1801 tccggcgccc aacgtgattc tgacgaagcc agaggtctca gaagggaccg aggtgacagt
     1861 gaagtgtgag gcccacccta gagccaaggt gacgctgaat ggggttccag cccagccact
     1921 gggcccgagg gcccagctcc tgctgaaggc caccccagag gacaacgggc gcagcttctc
     1981 ctgctctgca accctggagg tggccggcca gcttatacac aagaaccaga cccgggagct
     2041 tcgtgtcctg tatggccccc gactggacga gagggattgt ccgggaaact ggacgtggcc
     2101 agaaaattcc cagcagactc caatgtgcca ggcttggggg aacccattgc ccgagctcaa
     2161 gtgtctaaag gatggcactt tcccactgcc catcggggaa tcagtgactg tcactcgaga
     2221 tcttgagggc acctacctct gtcgggccag gagcactcaa ggggaggtca cccgcgaggt
     2281 gaccgtgaat gtgctctccc cccggtatga gattgtcatc atcactgtgg tagcagccgc
     2341 agtcataatg ggcactgcag gcctcagcac gtacctctat aaccgccagc ggaagatcaa
     2401 gaaatacaga ctacaacagg cccaaaaagg gacccccatg aaaccgaaca cacaagccac
     2461 gcctccctga acctatcccg ggacagggcc tcttcctcgg ccttcccata ttggtggcag
     2521 tggtgccaca ctgaacagag tggaagacat atgccatgca gctacaccta ccggccctgg
     2581 gacgccggag gacagggcat tgtcctcagt cagatacaac agcatttggg gccatgcttt
     2641 agagcacaat ggatctcgag ggatcttcca tacctaccag ttctgcgcct gcaggtcgcg
     2701 gccgcgactc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     2761 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     2821 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     2881 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     2941 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     3001 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     3061 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     3121 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa
     3181 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     3241 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     3301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcccgccat
     3421 ggtatcaacg ccatatttct atttacagta gggacctctt cgttgtgtag gtaccgctgt
     3481 attcctaggg aaatagtaga ggcaccttga actgtctgca tcagccatat agcccccgct
     3541 gttcgattta caaacacagg cacagtactg acaaacccat acacctcctc tgaaataccc
     3601 atagttgcta gggctgtctc cgaactcatt acaccctcca aagtcagagc tgtaatttcg
     3661 ccatcaaggg cagcgagggc ttctccagat aaaatagctt ctgccgagag tcccgtaagg
     3721 gtagacactt cagctaatcc ctcgatgagg tctactagaa tagtcagtgc ggctcccatt
     3781 ttgaaaattc acttacttga tcagcttcag aagatggcgg agggcctcca acacagtaat
     3841 tttcctcccg actcttaaaa tagaaaatgt caagtcagtt aagcaggaag tgactaactg
     3901 accgcagctg gccgtgcgac atcctctttt aattagttgc taggcaactg ccctccagag
     3961 ggcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca cccaggccta gaatgtttcc
     4021 acccaatcat tactatgaca acagctgttt tttttagtat taagcagagg ccgggggccc
     4081 ctggcctccg cttactctgg agaaaaagaa gagaggcatt gtagaggctt ccagaggcaa
     4141 cttgtcaaaa caggactgct tctatttctg tcacactgtc tggccctgtc acaaggtcca
     4201 gcacctccat acccccttta ataagcagtt tgggaacggg tgcgggtctt actccgccca
     4261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4321 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4381 gcttttttgg aggcctaggc ttttgcaaaa agctaattcg gcgtaatctg ctgcttgcaa
     4441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgctg
     4621 aagccagtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4681 aagagatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4981 ctatggaaaa acgccagcaa cgcaagctag cttctagcta gaaattgtaa acgttaatat
     5041 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     5101 aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga gtgttgttcc
     5161 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     5221 cgtctatcag ggcgatggcc gcccactacg tgaaccatca cccaaatcaa gttttttggg
     5281 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5341 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5401 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5461 tgcgccgcta cagggcgcgt actatggttg ctttgacgag cacgtataac gtgctttcct
     5521 cgttagaatc agagcgggag ctaaacagga ggccgattaa agggatttta gacaggaacg
     5581 gtacgccagc tggaccgcgg tctttcggac ttttgaaagt gatggtggtg ggggaaggat
     5641 tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc cgctcgggaa
     5701 ccccaccaca ggtaatgctt ttactggcct gctcccttat cgggaagcgg ggcgcatcat
     5761 atcaaatgac gcgccgctgt aaagtgttac gttgagaaag ctgctccctg cttgtgtgtt
     5821 ggaggtcgct g
//
