LOCUS       sequence-202038-        3927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F
  TITLE     LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance 
            and Neurotransmitter Release Properties at Excitatory Synapses along
            Cortical Axons.
  JOURNAL   PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul.
  PUBMED    27429220
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2016-07-18"; volume: "14(7):e1002516. doi"; pages: " 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul"
COMMENT     Sequence Label: pCAG mito-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..3927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..99
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             127..828
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(919..938)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    984..1039
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1038..1057)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1430..1446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1430..1446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1443..1465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1454..1470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1478..1484,1485..1502,1503..1508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1523..1544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1603..1798
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1649..1784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1804..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1841..1860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1895..1914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2006..2023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2177..2765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2257..2276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2936..3727,3728..3796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3559..3578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3797..3901)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattttggca aagaattgct cgagatgtcc gtcctgacgc cgctgctgct gcggggcttg
       61 acaggctcgg cccggcggct cccagtgccg cgcgccaaga tccattcgtt gggggatcca
      121 ccggtcatga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
      181 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      241 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      301 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
      361 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
      421 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
      481 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
      541 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
      601 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa gaccacatat
      661 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
      721 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
      781 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattaagc ggccgcaatt
      841 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
      901 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
      961 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     1021 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     1081 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     1141 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     1201 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     1261 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     1321 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcaccg
     1381 tcgaccagct ggtcgacggt gcaccgtcga ccagcttggc gtaatcatgg tcatagctgt
     1441 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1501 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1561 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     1621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1801 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1921 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1981 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2041 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2101 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2161 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2221 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2281 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2341 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2401 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2461 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2521 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2581 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2641 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2701 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2761 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2821 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2881 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2941 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3001 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3061 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3121 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3721 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3781 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3901 gcacatttcc ccgaaaagtg ccacctg
//
