LOCUS       sequence-202041-        4483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani 
            P, Raczkowski A, Petrey DS, Pon LA, Polleux F
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in 
            mammalian neurons.
  JOURNAL   Science. 2017 Nov 3;358(6363):623-630. doi: 10.1126/science.aan6009.
  PUBMED    29097544
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "623-630"
COMMENT     Sequence Label: pCAG G-CEPIA1er
FEATURES             Location/Qualifiers
     source          1..4483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     join(15..59,142..153)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(15..60,143..153)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             190..246
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     459..480
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(847..866)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             982..1422
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKDLGTVLRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVWDKDGNGYISAADL
                     RHVMTNLGEKLTDEEVDEMIREADIDGEGQVNYEEFVQMMTAK"
     CDS             1432..1461
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1498..1517)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1563..1618
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1617..1636)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1983..1999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1983..1999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1996..2018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2007..2023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2031..2037,2038..2055,2056..2061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2076..2097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2156..2351
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2202..2337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2357..2491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2394..2413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2448..2467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2559..2576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2730..3318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2810..2829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3489..4280,4281..4349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4112..4131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4350..4454)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgaattggc caccatggga tggagctgta tcatcctctt cttggtagca acagctacag
       61 gtaaggggtt aacagtagca ggcttgaggt ctggacatat atatgggtga caatgacatc
      121 cactttgcct ttctctccac aggcgcgcac tcccaggtcc aactgcaggg atccatggtc
      181 gactcttcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
      241 agctcactcg agaacgtcta tatcaaggcc gacgagcaga agaacggcat caaggcgtac
      301 ttcaagatcc gccacaacat cgagggcggc ggcgtgcagc tcgcctacca ctaccagcag
      361 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
      421 tccatacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      481 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
      541 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatccaggt cgagctggac
      601 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
      661 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      721 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      781 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
      841 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      901 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      961 aagctggagt acaacacgcg tgaccaactg actgaagagc agatcgcaga atttaaagag
     1021 gctttctccc tatttgacaa ggacggggat gggacgataa caaccaagga tctggggacg
     1081 gtgctgcggt ctctggggca gaaccccaca gaagcagagc tccaggacat gatcaatgaa
     1141 gtagatgccg acggtaatgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     1201 aaaatgaaag acacagacag tgaagaagaa attcgcgaag cgttccgtgt gtgggataag
     1261 gatggcaatg gctacatctc tgcagcagac cttcgccacg tgatgacaaa ccttggagag
     1321 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagatatcga tggagaaggt
     1381 caggtaaact acgaagagtt tgtacaaatg atgacagcga aggcggccgc agaacaaaaa
     1441 ctcatctcag aagaggatct gaatggggcc gcaagcgaga aggacgagct gtagagatct
     1501 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1561 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     1621 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1681 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1741 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1801 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1861 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1921 tgtccctctt ctcttatgga gatccctcga cctgcaccgt cgaccagctt ggcgtaatca
     1981 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2041 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2101 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     2161 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2221 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2281 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2341 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2461 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     2521 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2581 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2641 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2701 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3361 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3541 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3601 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3661 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3721 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3781 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3841 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3901 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3961 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4021 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4081 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4141 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4201 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4261 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4321 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4381 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4441 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggt
//
