LOCUS       sequence-202055-       10368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control lentivector. Coexpression of mTagBFP and gene of interest 
            from SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          42..107
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             237..257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    682..816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(719..738)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     773..792
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             984..1617
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1664..1789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2282..2515
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2700..2744
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3037..3154
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3294..3701
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3720..4418
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4428..4484
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             4771..4951
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6479..6498)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6712..6731)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6831..6853
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6891..6909)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6977..7081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7082..7150,7151..7942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7300..7319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8113..8701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8602..8621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8855..8872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8942..8962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8947..9276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9127..9262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
       61 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
      121 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
      181 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
      241 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
      301 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
      361 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
      421 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
      481 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
      541 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
      601 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      661 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      721 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      781 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
      841 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
      901 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
      961 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1021 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     1081 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1141 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     1201 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     1261 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     1321 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     1381 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     1441 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1501 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1561 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1621 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1681 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1741 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1801 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1861 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1921 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1981 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     2041 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     2101 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     2161 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     2221 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     2281 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     2341 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     2401 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     2461 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     2521 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     2581 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2641 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2701 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2761 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2821 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2881 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2941 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     3001 atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     3061 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     3121 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     3181 tccactttgg ctgatacgcg tctgcagccc cgataaaata aaagatttta tttagtctcc
     3241 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc
     3301 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
     3361 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
     3421 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     3481 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     3541 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     3601 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     3661 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gccgcgggct agcgccacca
     3721 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     3781 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     3841 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     3901 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     3961 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     4021 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     4081 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     4141 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     4201 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     4261 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     4321 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     4381 actgcgacct ccctagcaaa ctggggcaca agcttaatgg aagcggagct actaacttca
     4441 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctggatcc actagtgtcg
     4501 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4561 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     4621 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4681 aaagggggga ctggaagggc taattcactc ccaacgaaga caaaatcgtc gagagatgct
     4741 gcatataagc agctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttataataga
     5041 tttagcagga attgaactag gagtggagca cacaggcaaa gctgcagaag tacttggaag
     5101 aagccaccag agatactcac gattctgcac atacctggct aatcccagat cctaaggatt
     5161 acattaagtt tactaacatt tatataatga tttatagttt aaagtataaa cttatctaat
     5221 ttactattct gacagatatt aattaatcct caaatatcat aagagatgat tactattatc
     5281 cccatttaac acaagaggaa actgagaggg aaagatgttg aagtaatttt cccacaatta
     5341 cagcatccgt tagttacgac tctatgatct tctgacacaa attccattta ctcctcaccc
     5401 tatgactcag tcgaatatat caaagttatg gacattatgc taagtaacaa attacccttt
     5461 tatatagtaa atactgagta gattgagaga agaaattgtt tgcaaacctg aatagcttca
     5521 agaagaagag aagtgaggat aagaataaca gttgtcattt aacaagtttt aacaagtaac
     5581 ttggttagaa agggattcaa atgcataaag caagggataa atttttctgg caacaagact
     5641 atacaatata accttaaata tgacttcaaa taattgttgg aacttgataa aactaattaa
     5701 atattattga agattatcaa tattataaat gtaatttact tttaaaaagg gaacatagaa
     5761 atgtgtatca ttagagtaga aaacaatcct tattatcaca atttgtcaaa acaagtttgt
     5821 tattaacaca agtagaatac tgcattcaat taagttgact gcagattttg tgttttgtta
     5881 aaattagaaa gagataacaa caatttgaat tattgaaagt aacatgtaaa tagttctaca
     5941 tacgttcttt tgacatcttg ttcaatcatt gatcgaagtt ctttatcttg gaagaatttg
     6001 ttccaaagac tctgaaataa ggaaaacaat ctattatata gtctcacacc tttgttttac
     6061 ttttagtgat ttcaatttaa taatgtaaat ggttaaaatt tattcttctc tgagatcatt
     6121 tcacattgca gatagaaaac ctgagactgg ggtaattttt attaaaatct aatttaatct
     6181 cagaaacaca tctttattct aacatcaatt tttccagttt gatattatca tataaagtca
     6241 gccttcctca tctgcaggtt ccacaacaaa aatccaacca actgtggatc aaaaatattg
     6301 ggaaaaaatt aaaaatagca atacaacaat aaaaaaatac aaatcagaaa aacagcacag
     6361 tataacaact ttatttagca tttacaatct attaggtatt ataagtaatc tagccagatc
     6421 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     6481 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     6541 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6601 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     6661 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     6721 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     6781 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6841 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     6901 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6961 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7321 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8221 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8881 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     9301 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     9361 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     9421 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     9481 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     9541 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     9601 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     9661 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     9721 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     9781 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     9841 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     9901 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     9961 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
    10021 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
    10081 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
    10141 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
    10201 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
    10261 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
    10321 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtga
//
