LOCUS       sequence-202057-       11496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING HAQ and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STINGHAQ
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          2..67
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             197..217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    642..776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(679..698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     733..752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             944..1577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1624..1749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2242..2475
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2660..2704
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2997..3114
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3254..3661
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3680..4378
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4388..4444
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5859..6039
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7567..7586)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7800..7819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7919..7941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7979..7997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8065..8169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8170..8238,8239..9030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8388..8407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9201..9789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9690..9709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9943..9960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10030..10050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10035..10364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10215..10350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
       61 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
      121 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
      181 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
      241 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
      301 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
      361 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
      421 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
      481 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
      541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
      781 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
      841 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
      901 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
      961 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     1021 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     1081 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     1141 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     1201 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     1261 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     1321 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     1381 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1561 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1621 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1681 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1741 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1801 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1861 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1921 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1981 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     2041 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     2101 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     2161 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     2221 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     2281 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     2341 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     2401 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     2461 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     2521 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2581 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2641 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2701 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2761 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2821 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2881 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2941 agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaatttt
     3001 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     3061 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     3121 tattacaggg acagcagaga tccactttgg ctgatacgcg tctgcagccc cgataaaata
     3181 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     3241 caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga
     3301 gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct
     3361 gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc
     3421 cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt
     3481 aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt
     3541 gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta
     3601 taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg
     3661 gccgcgggct agcgccacca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     3721 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     3781 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     3841 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     3901 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     3961 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     4021 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     4081 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     4141 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc
     4201 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     4261 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     4321 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg
     4381 aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
     4441 acctggatcc atgccccact ccagcctgca tccatccatc ccgtgtccca ggggtcacgg
     4501 ggcccagaag gcagccttgg ttctgctgag tgcctgcctg gtgacccttt gggggctagg
     4561 agagccacca gagcacactc tccggtacct ggtgctccac ctagcctccc tgcagctggg
     4621 actgctgtta aacggggtct gcagcctggc tgaggagctg caccacatcc actccaggta
     4681 ccggggcagc tactggagga ctgtgcgggc ctgcctgggc tgccccctcc gccgtggggc
     4741 cctgttgctg ctgtccatct atttctacta ctccctccca aatgcggtcg gcccgccctt
     4801 cacttggatg cttgccctcc tgggcctctc gcaggcactg aacatcctcc tgggcctcaa
     4861 gggcctggcc ccagctgaga tctctgcagt gtgtgaaaaa gggaatttca acgtggccca
     4921 tgggctggca tggtcatatt acatcggata tctgcggctg atcctgccag agctccaggc
     4981 ccggattcga acttacaatc agcattacaa caacctgcta cggggtgcag tgagccagcg
     5041 gctgtatatt ctcctcccat tggactgtgg ggtgcctgat aacctgagta tggctgaccc
     5101 caacattcgc ttcctggata aactgcccca gcagaccgct gaccgtgctg gcatcaagga
     5161 tcgggtttac agcaacagca tctatgagct tctggagaac gggcagcggg cgggcacctg
     5221 tgtcctggag tacgccaccc ccttgcagac tttgtttgcc atgtcacaat acagtcaagc
     5281 tggctttagc cgggaggata ggcttgagca ggccaaactc ttctgccaga cacttgagga
     5341 catcctggca gatgcccctg agtctcagaa caactgccgc ctcattgcct accaggaacc
     5401 tgcagatgac agcagcttct cgctgtccca ggaggttctc cggcacctgc ggcaggagga
     5461 aaaggaagag gttactgtgg gcagcttgaa gacctcagcg gtgcccagta cctccacgat
     5521 gtcccaagag cctgagctcc tcatcagtgg aatggaaaag cccctccctc tccgcacgga
     5581 tttctcttga ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     5641 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     5701 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5761 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga
     5821 gagatgctgc atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     5881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6061 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6121 ataatagatt tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta
     6181 cttggaagaa gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc
     6241 taaggattac attaagttta ctaacattta tataatgatt tatagtttaa agtataaact
     6301 tatctaattt actattctga cagatattaa ttaatcctca aatatcataa gagatgatta
     6361 ctattatccc catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc
     6421 cacaattaca gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact
     6481 cctcacccta tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat
     6541 taccctttta tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa
     6601 tagcttcaag aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa
     6661 caagtaactt ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca
     6721 acaagactat acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa
     6781 ctaattaaat attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga
     6841 acatagaaat gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac
     6901 aagtttgtta ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg
     6961 ttttgttaaa attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata
     7021 gttctacata cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga
     7081 agaatttgtt ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt
     7141 tgttttactt ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg
     7201 agatcatttc acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa
     7261 tttaatctca gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata
     7321 taaagtcagc cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa
     7381 aaatattggg aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa
     7441 cagcacagta taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta
     7501 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     7561 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     7621 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     7681 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     7741 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     7801 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7861 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7921 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     7981 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8401 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8701 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10021 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
    10381 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
    10441 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
    10501 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
    10561 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
    10621 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
    10681 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    10741 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    10801 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    10861 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    10921 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    10981 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    11041 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    11101 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    11161 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    11221 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    11281 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    11341 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    11401 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    11461 attggacaaa ctacctacag agatttaaag ctctaa
//
