LOCUS       sequence-202060-       11437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING delta 342 and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STING-342STOP
FEATURES             Location/Qualifiers
     source          1..11437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5789..5969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7497..7516)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7730..7749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7849..7871
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7909..7927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7995..8099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8100..8168,8169..8960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8318..8337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9131..9719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9620..9639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9873..9890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9960..9980)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9965..10294
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10145..10280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catgccccac
     4501 tccagcctgc atccatccat cccgtgtccc aggggtcacg gggcccagaa ggcagccttg
     4561 gttctgctga gtgcctgcct ggtgaccctt tgggggctag gagagccacc agagcacact
     4621 ctccggtacc tggtcctcca cctagcctcc ctgcagctgg gactgctgtt aaacggggtc
     4681 tgcagcctgg ctgaggagct gcgccacatc cactccaggt accggggcag ctactggagg
     4741 actgtgcggg cctgcctggg ctgccccctc cgccgtgggg ccctgttgct gctgtccatc
     4801 tatttctact actccctccc aaatgcggtc ggcccgccct tcacttggat gcttgccctc
     4861 ctgggcctct cgcaggcact gaacatcctc ctgggcctca agggcctggc cccagctgag
     4921 atctctgcag tgtgtgaaaa agggaatttc aacgtggccc atgggctggc atggtcatat
     4981 tacatcggat atctgcggct gatcctgcca gagctccagg cccggattcg aacttacaat
     5041 cagcattaca acaacctgct acggggtgca gtgagccagc ggctgtatat tctcctccca
     5101 ttggactgtg gggtgcctga taacctgagt atggctgacc ccaacattcg cttcctggat
     5161 aaactgcccc agcagaccgg tgaccgtgct ggcatcaagg atcgggttta cagcaacagc
     5221 atctatgagc ttctggagaa cgggcagcgg gcgggcacct gtgtcctgga gtacgccacc
     5281 cccttgcaga ctttgtttgc catgtcacaa tacagtcaag ctggctttag ccgggaggat
     5341 aggcttgagc aggccaaact cttctgccgg acacttgagg acatcctggc agatgcctct
     5401 gagtctcaga acaactgccg cctcattgcc taccaggaac ctgcagatga cagcagcttc
     5461 tcgctgtccc aggaggttct ccggcacctg cggcaggagg aaaaggaaga ggttacttga
     5521 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5581 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5641 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5701 aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga gagatgctgc
     5761 atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5821 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5881 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5941 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6001 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt ataatagatt
     6061 tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta cttggaagaa
     6121 gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc taaggattac
     6181 attaagttta ctaacattta tataatgatt tatagtttaa agtataaact tatctaattt
     6241 actattctga cagatattaa ttaatcctca aatatcataa gagatgatta ctattatccc
     6301 catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc cacaattaca
     6361 gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact cctcacccta
     6421 tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat taccctttta
     6481 tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa tagcttcaag
     6541 aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa caagtaactt
     6601 ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca acaagactat
     6661 acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa ctaattaaat
     6721 attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga acatagaaat
     6781 gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac aagtttgtta
     6841 ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg ttttgttaaa
     6901 attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata gttctacata
     6961 cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga agaatttgtt
     7021 ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt tgttttactt
     7081 ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg agatcatttc
     7141 acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa tttaatctca
     7201 gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata taaagtcagc
     7261 cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa aaatattggg
     7321 aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa cagcacagta
     7381 taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta gccagatcct
     7441 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     7501 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     7561 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     7621 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     7681 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     7741 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7801 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7861 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7921 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7981 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8041 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8101 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8161 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8221 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8281 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8341 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8401 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8461 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8521 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8581 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8641 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8701 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8761 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8821 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8881 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8941 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9841 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9901 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     9961 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10021 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10201 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10261 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    10321 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    10381 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    10441 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    10501 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    10561 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    10621 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    10681 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    10741 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    10801 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    10861 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    10921 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    10981 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    11041 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    11101 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    11161 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    11221 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    11281 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    11341 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    11401 ctacctacag agatttaaag cttacttctg tggtgtg
//
