LOCUS       sequence-202061-       11421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector to express STING delta 354 and mTagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A STING-354STOP
FEATURES             Location/Qualifiers
     source          1..11421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          12..77
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             207..227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    652..786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(689..708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     743..762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             954..1587
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1634..1759
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2252..2485
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2670..2714
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3007..3124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3264..3671
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3690..4388
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4398..4454
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5794..5974
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7502..7521)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7735..7754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7854..7876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7914..7932)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8000..8104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8105..8173,8174..8965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8323..8342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9136..9724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9625..9644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9878..9895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9965..9985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9970..10299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10150..10285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
       61 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
      121 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
      181 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
      241 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
      301 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
      361 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
      421 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
      481 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
      541 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      601 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      661 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      721 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
      781 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
      841 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
      901 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
      961 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
     1021 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     1081 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     1141 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     1201 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     1261 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     1321 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     1381 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1441 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1501 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1561 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     1621 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1681 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1741 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1801 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1861 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1921 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1981 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     2041 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     2101 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     2161 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2221 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2281 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2341 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2401 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     2461 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2521 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2581 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2641 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2701 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2761 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2821 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2881 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2941 ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat
     3001 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     3061 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     3121 ttttcgggtt tattacaggg acagcagaga tccactttgg ctgatacgcg tctgcagccc
     3181 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     3241 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     3301 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     3361 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     3421 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     3481 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     3541 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     3601 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     3661 agtcgcccgg gccgcgggct agcgccacca tgagcgagct gattaaggag aacatgcaca
     3721 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     3781 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     3841 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     3901 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     3961 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     4021 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     4081 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     4141 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     4201 tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     4261 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     4321 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     4381 agcttaatgg aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg
     4441 agaaccctgg acctggatcc atgccccact ccagcctgca tccatccatc ccgtgtccca
     4501 ggggtcacgg ggcccagaag gcagccttgg ttctgctgag tgcctgcctg gtgacccttt
     4561 gggggctagg agagccacca gagcacactc tccggtacct ggtcctccac ctagcctccc
     4621 tgcagctggg actgctgtta aacggggtct gcagcctggc tgaggagctg cgccacatcc
     4681 actccaggta ccggggcagc tactggagga ctgtgcgggc ctgcctgggc tgccccctcc
     4741 gccgtggggc cctgttgctg ctgtccatct atttctacta ctccctccca aatgcggtcg
     4801 gcccgccctt cacttggatg cttgccctcc tgggcctctc gcaggcactg aacatcctcc
     4861 tgggcctcaa gggcctggcc ccagctgaga tctctgcagt gtgtgaaaaa gggaatttca
     4921 acgtggccca tgggctggca tggtcatatt acatcggata tctgcggctg atcctgccag
     4981 agctccaggc ccggattcga acttacaatc agcattacaa caacctgcta cggggtgcag
     5041 tgagccagcg gctgtatatt ctcctcccat tggactgtgg ggtgcctgat aacctgagta
     5101 tggctgaccc caacattcgc ttcctggata aactgcccca gcagaccggt gaccgtgctg
     5161 gcatcaagga tcgggtttac agcaacagca tctatgagct tctggagaac gggcagcggg
     5221 cgggcacctg tgtcctggag tacgccaccc ccttgcagac tttgtttgcc atgtcacaat
     5281 acagtcaagc tggctttagc cgggaggata ggcttgagca ggccaaactc ttctgccgga
     5341 cacttgagga catcctggca gatgcccctg agtctcagaa caactgccgc ctcattgcct
     5401 accaggaacc tgcagatgac agcagcttct cgctgtccca ggaggttctc cggcacctgc
     5461 ggcaggagga aaaggaagag gttactgtgg gcagcttgaa gacctcagcg gtgcccagta
     5521 cctgactcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5581 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5641 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5701 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaaaatc gtcgagagat
     5761 gctgcatata agcagctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5941 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6001 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat
     6061 agatttagca ggaattgaac taggagtgga gcacacaggc aaagctgcag aagtacttgg
     6121 aagaagccac cagagatact cacgattctg cacatacctg gctaatccca gatcctaagg
     6181 attacattaa gtttactaac atttatataa tgatttatag tttaaagtat aaacttatct
     6241 aatttactat tctgacagat attaattaat cctcaaatat cataagagat gattactatt
     6301 atccccattt aacacaagag gaaactgaga gggaaagatg ttgaagtaat tttcccacaa
     6361 ttacagcatc cgttagttac gactctatga tcttctgaca caaattccat ttactcctca
     6421 ccctatgact cagtcgaata tatcaaagtt atggacatta tgctaagtaa caaattaccc
     6481 ttttatatag taaatactga gtagattgag agaagaaatt gtttgcaaac ctgaatagct
     6541 tcaagaagaa gagaagtgag gataagaata acagttgtca tttaacaagt tttaacaagt
     6601 aacttggtta gaaagggatt caaatgcata aagcaaggga taaatttttc tggcaacaag
     6661 actatacaat ataaccttaa atatgacttc aaataattgt tggaacttga taaaactaat
     6721 taaatattat tgaagattat caatattata aatgtaattt acttttaaaa agggaacata
     6781 gaaatgtgta tcattagagt agaaaacaat ccttattatc acaatttgtc aaaacaagtt
     6841 tgttattaac acaagtagaa tactgcattc aattaagttg actgcagatt ttgtgttttg
     6901 ttaaaattag aaagagataa caacaatttg aattattgaa agtaacatgt aaatagttct
     6961 acatacgttc ttttgacatc ttgttcaatc attgatcgaa gttctttatc ttggaagaat
     7021 ttgttccaaa gactctgaaa taaggaaaac aatctattat atagtctcac acctttgttt
     7081 tacttttagt gatttcaatt taataatgta aatggttaaa atttattctt ctctgagatc
     7141 atttcacatt gcagatagaa aacctgagac tggggtaatt tttattaaaa tctaatttaa
     7201 tctcagaaac acatctttat tctaacatca atttttccag tttgatatta tcatataaag
     7261 tcagccttcc tcatctgcag gttccacaac aaaaatccaa ccaactgtgg atcaaaaata
     7321 ttgggaaaaa attaaaaata gcaatacaac aataaaaaaa tacaaatcag aaaaacagca
     7381 cagtataaca actttattta gcatttacaa tctattaggt attataagta atctagccag
     7441 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     7501 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     7561 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     7621 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     7681 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     7741 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     7801 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7861 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     7921 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10201 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    10321 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
    10381 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
    10441 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
    10501 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
    10561 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
    10621 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
    10681 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
    10741 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
    10801 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
    10861 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
    10921 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
    10981 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
    11041 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
    11101 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
    11161 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
    11221 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
    11281 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    11341 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
    11401 acaaactacc tacagagatt t
//
