LOCUS       sequence-202081-        3530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. solfataricus PCNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Iwata F, Hirakawa H, Nagamune T
  TITLE     Three proliferating cell nuclear antigen homologues from 
            Metallosphaera sedula form a head-to-tail heterotrimer.
  JOURNAL   Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588.
  PUBMED    27228945
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-27"; volume: "6:26588. doi"; pages: " 
            10.1038/srep26588"
COMMENT     Sequence Label: pMD-SsoPCNA1
FEATURES             Location/Qualifiers
     source          1..3530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1065
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIGESLHACRSTLEDLLVIWIGMPWESHMMFKIV
                     YPNAKDFFSFINSITNVTDSIILNFTEDGIFSRHLTEDKVLMAIMRIPKDVLSEYSIDS
                     PTSVKLDVSSVKKILSKASSKKATIELTETDSGLKIIIRDEKSGAKSTIYIKAEKGQVE
                     QLTEPKVNLAVNFTTDESVLNVIAADVTLVGEEMRISTEEDKIKIEAGEEGKRYVAFLM
                     KDKPLKELSIDTSASSSYSAEMFKDAVKGLRGFSAPTMVSFGENLPMKIDVEAVSGGHM
                     IFWIAPRLSGSGMPWNRIPGYRARIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIA
                     KRPAPIALPNSCAA"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(920..937)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(920..936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(929..951)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1144..1163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1263..1285
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1323..1341)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1409..1513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1514..1582,1583..2374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1732..1751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2545..3133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3034..3053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3287..3304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3421..3442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3457..3462,3463..3480,3481..3487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3495..3511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3500..3522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3519..3530,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3519..3530,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag ctatttaggt gacactatag gggaaagctt gcatgcctgc
       61 aggtcgactc tagaggatct actagtcata tggattggca tgccatggga atcccatatg
      121 atgtttaaaa ttgtgtatcc gaacgccaaa gatttcttta gcttcatcaa cagcattacc
      181 aacgttacgg attctatcat cctgaacttc accgaagatg gcatcttcag tcgtcatctg
      241 acggaagata aagtgctgat ggcaatcatg cgcattccga aagatgttct gtctgaatac
      301 agtattgata gcccgacctc tgtgaaactg gatgtgagct ctgtgaagaa aattctgagc
      361 aaagcaagta gcaaaaaagc gacgattgaa ctgaccgaaa cggattctgg cctgaaaatc
      421 atcatccgtg atgaaaaatc tggtgccaaa agtaccatct acatcaaagc agaaaaaggc
      481 caggttgaac agctgacgga accgaaagtg aacctggccg ttaattttac cacggatgaa
      541 agcgtgctga acgttatcgc ggccgatgtg accctggttg gtgaagaaat gcgtatcagc
      601 acggaagaag ataaaatcaa aattgaagcg ggcgaagaag gtaaacgcta tgtggccttt
      661 ctgatgaaag ataaaccgct gaaagaactg agcatcgata cctctgcctc tagtagctac
      721 agcgcagaaa tgttcaaaga tgcggtgaaa ggcctgcgtg gttttagtgc gccgacgatg
      781 gtgtctttcg gtgaaaatct gccgatgaaa attgatgtgg aagcagttag cggcggtcac
      841 atgatcttct ggattgcgcc gcgcctgtcg ggatccggca tgccatggaa tcggatcccc
      901 gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      961 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1021 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggccct
     1081 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat agtgcactct
     1141 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     1201 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     1261 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     1321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3181 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3241 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3301 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3361 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3421 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3481 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
//
