LOCUS       sequence-202097-       14688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of CD1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14688)
  AUTHORS   Manolova V, Hirabayashi Y, Mori L, De Libero G
  TITLE     CD1a and CD1b surface expression is independent from de novo 
            synthesized glycosphingolipids.
  JOURNAL   Eur J Immunol. 2003 Jan;33(1):29-37.
  PUBMED    12594829
REFERENCE   2  (bases 1 to 14688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol."; date: "2003-01"; volume: "33(1)"; pages: "29-37"
COMMENT     Sequence Label: pHCD1B
FEATURES             Location/Qualifiers
     source          1..14688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             910..918
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1680..1696)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1680..1696)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1787..1806)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1852..1907
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1906..1925)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3239..3633
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(10377..10396)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10681..10698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10852..11440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10932..10951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11611..12402,12403..12471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12234..12253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12472..12576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12644..12662
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13115..13260
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13317..14111
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13371..13390)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13981..14000
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(14305..14324)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14349..14396
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgacatgc tgctgctgcc atttcaactg ttagctgttc tctttcctgg
      721 tggtaacagt gaacatgcct tccaggggcc gacctccttt catgttatcc agacctcgtc
      781 ctttaccaat agtacctggg cacaaactca aggctcaggc tggttggatg atttgcagat
      841 tcatggctgg gatagcgact caggcactgc catattcctg aagccttggt ctaaaggtaa
      901 ctttagtgat aaggaggttg ctgagttaga ggagatattc cgagtctaca tctttggatt
      961 cgctcgagaa gtacaagact ttgccggtga tttccagatg aaatacccct ttgagatcca
     1021 gggcatagca ggctgtgagc tacattctgg aggtgccata gtaagcttcc tgaggggagc
     1081 tctaggagga ttggatttcc tgagtgtcaa gaatgcttca tgtgtgcctt ccccagaagg
     1141 tggcagcagg gcacagaaat tctgtgcact aatcatacaa tatcaaggta tcatggaaac
     1201 tgtgagaatt ctcctctatg aaacctgccc ccgatatctc ttgggcgtcc tcaatgcagg
     1261 aaaagcagat ctgcaaagac aagtgaagcc tgaggcctgg ctgtccagtg gccccagtcc
     1321 tggacctggc cgtctgcagc ttgtgtgcca tgtctcagga ttctacccaa agcccgtgtg
     1381 ggtgatgtgg atgcggggtg agcaggagca gcagggcact cagctagggg acatcctgcc
     1441 caatgctaac tggacatggt atctccgagc aaccctggat gtggcagatg gggaggcggc
     1501 tggcctgtcc tgtcgggtga agcacagcag tttagagggc caggacatca tcctctactg
     1561 gagaaacccc acctccattg gctcaattgt tttggcaata atagtgcctt ccttgctcct
     1621 tttgctatgc cttgcattat ggtatatgag gcgccggtca tatcagaata tcccatgagg
     1681 atccactagt tctagagcgg ccgcaattca ctcctcaggt gcaggctgcc tatcagaagg
     1741 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     1801 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     1861 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     1921 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     1981 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     2041 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     2101 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     2161 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     2221 tcttatggag atccctcgac ggatcctgag aacttcaggg tgagtctatg ggacccttga
     2281 tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac
     2341 agggtacagt ttagaatggg aaacagacga atgattgcat cagtgtggaa gtctcaggat
     2401 cgttttagtt tcttttattt gctgttcata acaattgttt tcttttgttt aattcttgct
     2461 ttcttttttt ttcttctccg caatttttac tattatactt aatgccttaa cattgtgtat
     2521 aacaaaagga aatatctctg agatacatta agtaacttaa aaaaaaactt tacacagtct
     2581 gcctagtaca ttactatttg gaatatatgt gtgcttattt gcatattcat aatctcccta
     2641 ctttattttc ttttattttt aattgataca taatcattat acatatttat gggttaaagt
     2701 gtaatgtttt aatatgtgta cacatattga ccaaatcagg gtaattttgc atttgtaatt
     2761 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
     2821 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
     2881 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
     2941 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
     3001 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
     3061 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
     3121 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa ttcaccccac cagtgcaggc
     3181 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
     3241 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
     3301 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
     3361 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
     3421 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
     3481 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
     3541 cagcccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
     3601 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
     3661 tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag
     3721 ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag
     3781 atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta
     3841 cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc
     3901 tcccccactc acagtgaccc ggaatctgca gtgctagtct cccggaacta tcactctttc
     3961 acagtctgct ttggaaggac tgggcttagt atgaaaagtt aggactgaga agaatttgaa
     4021 agggggcttt ttgtagcttg atattcacta ctgtcttatt accctatcat aggcccaccc
     4081 caaatggaag tcccattctt cctcaggatg tttaagatta gcattcagga agagatcaga
     4141 ggtctgctgg ctcccttatc atgtccctta tggtgcttct ggctctgcag ttattagcat
     4201 agtgttacca tcaaccacct taacttcatt tttcttattc aatacctagg taggtagatg
     4261 ctagattctg gaaataaaat atgagtctca agtggtcctt gtcctctctc ccagtcaaat
     4321 tctgaatcta gttggcaaga ttctgaaatc aaggcatata atcagtaata agtgatgata
     4381 gaagggtata tagaagaatt ttattatatg agagggtgaa atcccagcaa tttgggaggc
     4441 tgaggcagga gaatcgcttg atcctgggag gcagaggttg cagtgagcca agattgtgcc
     4501 actgcattcc agcccaggtg acagcatgag actccgtcac aaaaaaaaaa gaaaaaaaag
     4561 gggggggggg gcggtggagc caagatgacc gaataggaac agctccagtc tatagctccc
     4621 atcgtgagtg acgcagaaga cgggtgattt ctgcatttcc aactgaggta ccaggttcat
     4681 ctcacaggga agtgccaggc agtgggtgca ggacagtagg tgcagtgcac tgtgcatgag
     4741 ccgaagcagg gcgaggcatc acctcacccg ggaagcacaa ggggtcaggg aattcccttt
     4801 cctagtcaaa gaaaagggtg acagatggca cctggaaaat cgggtcactc ccgccctaat
     4861 actgcgctct tccaacaagc tttctttgga cttagatcaa tttcccttgg gaagaagatt
     4921 tttagcacag caaggggcag gatgttcaac tgtgagaaaa cgaagaatta gccaaaaaac
     4981 ttccagtaag cctgcaaaaa aaaaaaaaaa ataaaagcta agtttctata aatgttctgt
     5041 aaatgtaaaa cagaaggtaa gtcaactgca cctaataaaa atcacttaat agcaatgtgc
     5101 tgtgtcagtt gtttattgga accacacccg gtacacatcc tgtccagcat ttgcagtgcg
     5161 tgcattgaat tattgtgctg gctagacttc atggcgcctg gcaccgaatc ctgccttctc
     5221 agccaaaatg aataattgct ttgttggcaa gaaactaagc atcaatggga cgcgtgcaaa
     5281 gcaccggcgg cggtagatgc ggggtaagta ctgaatttta attcgaccta tcccggtaaa
     5341 gcgaaagcga cacgcttttt tttcacacat agcgggaccg aacacgttat aagtatcgat
     5401 taggtctatt tttgtctctc tgtcggaacc agaactggta aaagtttcca ttgcgtctgg
     5461 gcttgtctat cattgcgtct ctatggtttt tggaggatta gacgggccac cagtaatggt
     5521 gcatagcgga tgtctgtacc gccatcggtg caccgatata ggtttggggc tccccaaggg
     5581 actgctggga tgacagcttc atattatatt gaatgggcgc ataatcagct taattggtga
     5641 ggacaagcta caagttgtaa cctgatctcc acaaagtacc gttgccggtc ggggtcaaac
     5701 cgtcttcggt gctcgaaacc gccttaaact acagacaggt cccagccaag taggcggatc
     5761 aaaacctcaa aaaggcggga gccaatcaaa atgcagcatt atattttaag ctcaccgaaa
     5821 ccggtaagta aagactatgt attttttccc agtgaataat tgttgttaac aataatcaca
     5881 ccatcaccgt tttttcaagc gggaaaaaat agccagctaa ctataaaaag ctgctgacag
     5941 accccggttt tcacatggac ctgaaacctt ttgcaagaac caatccattc tcagggttgg
     6001 attgtctgtg gtgcagagag cctcttacag aagttgatgc ttttaggtgc atggtcaaag
     6061 actttcatgt tgtaattcgg gaaggctgta gatatggtgc atgtaccatt tgtcttgaaa
     6121 actgtttagc tactgaaaga agactttggc aaggtgttcc agtaacaggt gaggaagctg
     6181 aattattgca tggcaaaaca cttgataggc tttgcataag atgctgctac tgtgggggca
     6241 aactaacaaa aaatgaaaaa catcggcatg tgctttttaa tgagcctttc tgcaaaacca
     6301 gagctaacat aattagagga cgctgctacg actgctgcag acatggttca aggtccaaat
     6361 acccatagaa acttggatga ttcacctgca ggaccgttgc tgattttaag tccatgtgca
     6421 ggcacaccta ccaggtctcc tgcagcacct gatgcacctg atttcagact tccgtgccat
     6481 ttcggccgtc ctactaggaa gcgaggtccc actacgcctc cgctttcctc tcccggaaaa
     6541 ctgtgtgcaa cagggccacg tcgagtgtat tctgtgactg tctgctgtgg aaactgcgga
     6601 aaagagctga cttttgctgt gaagaccagc tcgacgtccc tgcttggatt tgaacacctt
     6661 ttaaactcag atttagacct cttgtgtcca cgttgtgaat ctcgcgagcg tcatggcaaa
     6721 cgataaaggt agcaattggg attcgggctt gggatgctca tatctgctga ctgaggcaga
     6781 atgtgaaagt gacaaagaga atgaggaacc cggggcaggt gtagaactgt ctgtggaatc
     6841 tgatcggtat gatagccagg atgaggattt tgttgacaat gcatcagtct ttcagggaaa
     6901 tcacctggag gtcttccagg cattagagaa aaaggcgggt gaggagcaga ttttaaattt
     6961 gaaaagaaaa gtattgggga gttcgcaaaa cagcagcggt tccgaagcat ctgaaactcc
     7021 agttaaaaga cggaaatcag gagcaaagcg aagattattt gctgaaaatg aagctaaccg
     7081 tgttcttacg cccctccagg tacaggggga gggggagggg aggcaagaac ttaatgagga
     7141 gcaggcaatt agtcatctac atctgcagct tgttaaatct aaaaatgcta cagtttttaa
     7201 gctggggctc tttaaatctt tgttcctttg tagcttccat gatattacga ggttgtttaa
     7261 gaatgataag accactaatc agcaatgggt gctggctgtg tttggccttg cagaggtgtt
     7321 ttttgaggcg agtttcgaac tcctaaagaa gcagtgtagt tttctgcaga tgcaaaaaag
     7381 atctcatgaa ggaggaactt gtgcagttta cttaatctgc tttaacacag ctaaaagcag
     7441 agaaacagtc cggaatctga tggcaaacat gctaaatgta agagaagagt gtttgatgct
     7501 gcagccacct aaaattcgag gactcagcgc agctctattc tggtttaaaa gtagtttgtc
     7561 acccgctaca cttaaacatg gtgctttacc tgagtggata cgggcgcaaa ctactctgaa
     7621 cgagagcttg cagaccgaga aattcgactt cggaactatg gtgcaatggg cctatgatca
     7681 caaatatgct gaggagtcta aaatagccta tgaatatgct ttggctgcag gatctgatag
     7741 caatgcacgg gcttttttag caactaacag ccaagctaag catgtgaagg actgtgcaac
     7801 tatggtaaga cactatctaa gagctgaaac acaagcatta agcatgcctg catatattaa
     7861 agctaggtgc aagctggcaa ctggggaagg aagctggaag tctatcctaa ctttttttaa
     7921 ctatcagaat attgaattaa ttacctttat taatgcttta aagctctggc taaaaggaat
     7981 tccaaaaaaa aactgtttag catttattgg ccctccaaac acaggcaagt ctatgctctg
     8041 caactcatta attcattttt tgggtggtag tgttttatct tttgccaacc ataaaagtca
     8101 cttttggctt gcttccctag cagatactag agctgcttta gtagatgatg ctactcatgc
     8161 ttgctggagg tactttgaca catacctcag aaatgcattg gatggctacc ctgtcagtat
     8221 tgatagaaaa cacaaagcag cggttcaaat taaagctcca cccctcctgg taaccagtaa
     8281 tattgatgtg caggcagagg acagatattt gtacttgcat agtcgggtgc aaacctttcg
     8341 ctttgagcag ccatgcacag atgaatcggg tgagcaacct tttaatatta ctgatgcaga
     8401 ttggaaatct ttttttgtaa ggttatgggg gcgtttagac ctgattgacg aggaggagga
     8461 tagtgaagag gatggagaca gcatgcgaac gtttacatgt agcgcaagaa acacaaatgc
     8521 agttgattga gaaaagtagt gataagttgc aagatcatat actgtactgg actgctgtta
     8581 gaactgagaa cacactgctt tatgctgcaa ggaaaaaagg ggtgactgtc ctaggacact
     8641 gcagagtacc acactctgta gtttgtcaag agagagccaa gcaggccatt gaaatgcagt
     8701 tgtctttgca ggagttaagc aaaactgagt ttggggatga accatggtct ttgcttgaca
     8761 caagctggga ccgatatatg tcagaaccta aacggtgctt taagaaaggc gccagggtgg
     8821 tagaggtgga gtttgatgga aatgcaagca atacaaactg gtacactgtc tacagcaatt
     8881 tgtacatgcg cacagaggac ggctggcagc ttgcgaaggc tggggctgac ggaactgggc
     8941 tctactactg caccatggcc ggtgctggac gcatttacta ttctcgcttt ggtgacgagg
     9001 cagccagatt tagtacaaca gggcattact ctgtaagaga tcaggacaga gtgtatgctg
     9061 gtgtctcatc cacctcttct gattttagag atcgcccaga cggagtctgg gtcgcatccg
     9121 aaggacctga aggagaccct gcaggaaaag aagccgagcc agcccagcct gtctcttctt
     9181 tgctcggctc ccccgcctgc ggtcccatca gagcaggcct cggttgggta cgggacggtc
     9241 ctcgctcgca cccctacaat tttcctgcag gctcgggggg ctctattctc cgctcttcct
     9301 ccaccccggt gcagggcacg gtaccggtgg acttggcatc aaggcaggaa gaagaggagc
     9361 agtcgcccga ctccacagag gaagaaccag tgactctccc aaggcgcacc accaatgatg
     9421 gattccacct gttaaaggca ggagggtcat gctttgctct aatttcagga actgctaacc
     9481 aggtaaagtg ctatcgcttt cgggtgaaaa agaaccatag acatcgctac gagaactgca
     9541 ccaccacctg gttcacagtt gctgacaacg gtgctgaaag acaaggacaa gcacaaatac
     9601 tgatcacctt tggatcgcca agtcaaaggc aagactttct gaaacatgta ccactacctc
     9661 ctggaatgaa catttccggc tttacagcca gcttggactt ctgatcactg ccattgcctt
     9721 ttcttcatct gactggtgta ctatgccaaa tctatggttt ctattgttct tgggactagt
     9781 tgctgcaatg caactgctgc tattactgtt cttactcttg ttttttcttg tatactggga
     9841 tcattttgag tgctcctgta caggtctgcc cttttaatgc ctttacatca ctggctattg
     9901 gctgtgtttt tactgttgtg tggatttgtt ttatatactg tatgaagttt tttcatttgt
     9961 gcttgtattg ctgtttgtaa gttttttact agagtttgta ttccccctgc tcagatttta
    10021 tatggtttaa gctgcagcaa taaaaatgag tgcacgaaaa agagtaaaac gtgccagtgc
    10081 ctatgacctg tacaggacat gcaagcaagc gggcacatgt ccaccagatg tgataccaaa
    10141 ggtagaagga gatactatag cagataaaat tttgaaattt gggggtcttg caatctactt
    10201 aggagggcta ggaataggaa catggtctac tggaagggtt gctgcaggtg gatcaccaag
    10261 gtacacacca ctccgaacag cagggtccac atcatcgctt gcatcaatag gatcgatcct
    10321 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
    10381 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
    10441 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
    10501 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
    10561 cctaatgcag gagtcgcata agggagagcg tcgagcggct gcgcggccgg ccgctatgcg
    10621 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11281 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    11641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
    11941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    12061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    12121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    12181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
    12241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    12301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    12361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    12421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    12481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    12541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    12601 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    12661 gccctttcgt cttcaagaat tctcatgttt gacagcttat catcgataag cttcacgctg
    12721 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
    12781 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
    12841 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
    12901 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
    12961 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
    13021 gggatcaaga tcctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
    13081 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
    13141 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc
    13201 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
    13261 cagcgtcaac agcgtgccgc agatctgatc aagagacagg atgaggatcg tttcgcatga
    13321 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
    13381 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
    13441 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
    13501 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
    13561 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
    13621 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
    13681 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
    13741 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
    13801 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
    13861 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
    13921 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
    13981 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
    14041 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
    14101 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
    14161 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    14221 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    14281 ccccgggaga tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    14341 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
    14401 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    14461 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    14521 agggctcgca gccaacgtcg gggcggcaag ccctgccata gccacgggcc ccgtgggtta
    14581 gggacggcgg atcgcggccc tctagagagc ttggcccatt gcatacgttg tatccatatc
    14641 ataatatgta catttatatt ggctcatgtc caacattacc gccatgtt
//
