LOCUS       sequence-202099-       12130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 12130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033."
COMMENT     Sequence Label: pSMPP-mCherry-mTrim21
FEATURES             Location/Qualifiers
     source          1..12130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             91..724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    771..896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1393..1626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1811..1855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2140..2257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2410..2817
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2856..2865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2862..3569
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3007..3025)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3275..3294
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3606..4994
                     /codon_start=1
                     /product="mouse RING E3 ubiquitin ligase that can be used 
                     together with antibodies to degrade intracellular proteins 
                     (Clift et al., 2018)"
                     /label=TRIM21
                     /note="binds to the Fc domain of antibodies"
                     /translation="MSLEKMWEEVTCSICLDPMVEPMSIECGHCFCKECIFEVGKNGGS
                     SCPECRQQFLLRNLRPNRHIANMVENLKQIAQNTKKSTQETHCMKHGEKLHLFCEEDGQ
                     ALCWVCAQSGKHRDHTRVPIEEAAKVYQEKIHVALEKLRKGKELAEKMEMDLTMQRTDW
                     KRNIDTQKSRIHAEFALQNSLLAQEEQRQLQRLEKDQREYLRLLGKKEAELAEKNQALQ
                     ELISELERRIRGSELELLQEVRIILERSGSWNLDTLDIDAPDLTSTCPVPGRKKMLRTC
                     WVHITLDRNTANSWLIISKDRRQVRMGDTHQNVSDNKERFSNYPMVLGAQRFSSGKMYW
                     EVDVTQKEAWDLGVCRDSVQRKGQFSLSPENGFWTIWLWQDSYEAGTSPQTTLHIQVPP
                     CQIGIFVDYEAGVVSFYNITDHGSLIYTFSECVFAGPLRPFFNVGFNYSGGNAAPLKLC
                     PLKM"
     primer_bind     complement(5107..5127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5525..5536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5568..6067
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5603..5626)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6000..6019
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6088..6687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6088..6107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6584..6604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6782..7015
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7040..7058)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7041..7058)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7348..7367)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7581..7600)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7700..7722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7760..7778)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7846..7950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7951..8019,8020..8811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8169..8188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8982..9570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9471..9490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9724..9741
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9811..9831)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9816..10145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9996..10131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11279..11344
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11474..11494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11919..12053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11956..11975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12010..12029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
       61 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
      121 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
      181 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
      241 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
      301 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
      361 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
      421 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
      481 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
      541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      721 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      781 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      841 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      901 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      961 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1021 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1081 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1141 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1201 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     1261 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1321 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1381 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1441 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1501 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1561 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1621 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1681 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1741 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1801 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1861 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1921 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1981 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2041 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2101 atctcgacgg tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2161 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2221 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2281 agatccagtt tggatcgata agcttgatat cgaattcctg cagccccgat aaaataaaag
     2341 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     2401 ctagctgcag taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag
     2461 ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg
     2521 tgagcagttt cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg
     2581 gcccgaggcc aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga
     2641 cccatcagat gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat
     2701 taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa
     2761 agagctcaca acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggggg
     2821 gatccactgt gatcatcaga cgcgtacagc atatggccac catggtgagc aagggcgagg
     2881 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     2941 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3001 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     3061 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     3121 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3181 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     3241 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     3301 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     3361 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     3421 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     3481 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     3541 ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga
     3601 attcaatgtc tctggaaaag atgtgggagg aggtcacctg ttctatctgc ctggatccca
     3661 tggtggagcc tatgagtatc gaatgtggcc attgcttttg caaggaatgc atttttgaag
     3721 ttgggaagaa tgggggcagt tcatgtcccg agtgccggca acagtttctg ctccgaaacc
     3781 tcaggcccaa tagacatata gccaacatgg tggaaaacct taaacagata gcccagaata
     3841 ccaagaagag tacccaggaa acgcactgca tgaagcatgg agagaagctt cacctattct
     3901 gtgaggaaga tgggcaggcc ctttgctggg tgtgtgccca gtctgggaaa caccgggacc
     3961 acaccagggt ccctattgaa gaggctgcta aggtatacca ggagaagatc cacgtggctt
     4021 tagaaaaact gagaaagggg aaagagttgg ccgagaagat ggaaatggat ctcacgatgc
     4081 aaagaacaga ctggaagagg aacattgaca cccagaagtc gaggattcac gcagagttcg
     4141 cacttcagaa tagcttgctg gctcaggagg agcagaggca gctgcagagg ctggagaagg
     4201 atcaaaggga gtacctgaga ctcctgggga agaaggaggc tgagctggct gagaagaacc
     4261 aggccctgca ggagctgatc tcagagctgg agaggaggat tcgtggttca gagctggagc
     4321 tactgcagga ggtgaggatc atcctggaaa ggagtggatc ctggaacctg gacacgttag
     4381 atattgacgc cccagaccta acaagcacat gccctgtgcc agggcggaag aagatgctga
     4441 ggacgtgttg ggttcatatt actctggatc gcaacaccgc caactcatgg ctcatcatct
     4501 caaaggatcg gagacaagtg aggatgggag acacccatca gaacgtgtct gacaataagg
     4561 agaggtttag taattacccc atggtgctag gtgcccagag attctcctct gggaagatgt
     4621 actgggaggt agatgtgact caaaaggagg cctgggatct gggggtttgc agagattctg
     4681 ttcagaggaa agggcagttt tcactcagtc ccgagaatgg cttctggacc atttggttat
     4741 ggcaagacag ctatgaggct ggtaccagtc ctcagaccac cctccacatt caagtacctc
     4801 catgccaaat tgggatcttt gtggactatg aggctggcgt tgtctccttc tacaacataa
     4861 ctgaccatgg ctccctcatt tacaccttct cggagtgtgt ttttgctgga cctctgcgac
     4921 ctttcttcaa tgttggtttc aattatagtg ggggaaatgc agcgcctcta aagctctgtc
     4981 cactaaagat gtgagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     5041 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5101 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5161 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5221 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5281 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5341 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5401 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5461 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5521 cccttcggcc ctcaatccag cggaccttcc ttcccatcaa ttctaccggg taggggaggc
     5581 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5641 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5701 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5761 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5821 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5881 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5941 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6001 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6061 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6121 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6181 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6241 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6301 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6361 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6421 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6481 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6541 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6601 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6661 tgcatgaccc gcaagcccgg tgcctgactc gagggaatta attcgagctc ggtaccttta
     6721 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6781 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     7021 tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     7081 ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat
     7141 ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg
     7201 ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac
     7261 caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
     7321 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     7381 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     7441 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     7501 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     7561 gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     7621 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7681 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7741 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7801 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7921 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8041 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8101 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8161 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8221 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8281 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8341 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8401 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8461 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8521 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8581 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8641 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8701 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8761 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8821 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8881 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8941 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9001 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9061 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9121 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9361 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9601 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9661 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9721 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     9781 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     9841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9961 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    10021 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10081 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10141 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
    10201 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
    10261 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
    10321 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
    10381 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
    10441 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
    10501 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
    10561 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
    10621 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
    10681 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
    10741 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
    10801 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
    10861 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
    10921 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
    10981 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
    11041 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
    11101 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
    11161 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
    11221 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
    11281 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    11341 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    11401 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    11461 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    11521 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    11581 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
    11641 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
    11701 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
    11761 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
    11821 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    11881 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    11941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    12061 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    12121 attacctgtg
//
