LOCUS       sequence-202100-       10019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Transfer Plasmid empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033."
COMMENT     Sequence Label: pSMPP
FEATURES             Location/Qualifiers
     source          1..10019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             141..774
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    821..946
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2190..2307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2460..2867
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3046..3066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3464..3475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3507..4006
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3542..3565)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3939..3958
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4027..4626
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4027..4046)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4523..4543
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4721..4954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4979..4997)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4980..4997)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5287..5306)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5520..5539)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5639..5661
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5699..5717)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5785..5889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5890..5958,5959..6750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6108..6127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6921..7509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7410..7429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7663..7680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7750..7770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7755..8084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7935..8070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9218..9283
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9413..9433
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9858..9992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9895..9914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9949..9968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
       61 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
      121 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
      181 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
      241 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
      301 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
      361 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
      421 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
      481 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
      541 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
      601 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      661 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      721 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      781 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      841 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      901 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      961 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1021 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1081 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1141 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1201 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1261 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2161 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2221 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2281 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2341 tggatcgata agcttgatat cgaattcctg cagccccgat aaaataaaag attttattta
     2401 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag
     2461 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
     2521 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     2581 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     2641 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     2701 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     2761 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     2821 acccctcact cggcgcgcca gtcctccgac agactgagtc gcccgggggg gatccactgt
     2881 gatcatcaga cgcgtacagc atatgtcagt taattaaact ggtttaaact caggcggccg
     2941 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     3001 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3061 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3121 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3181 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3241 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3301 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3361 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3421 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3481 ggaccttcct tcccatcaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     3541 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     3601 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     3661 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     3721 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     3781 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     3841 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     3901 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     3961 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     4021 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4081 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4141 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     4201 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     4261 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     4321 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     4381 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     4441 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     4501 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     4561 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     4621 gcctgactcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     4681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4741 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4921 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     4981 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     5041 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     5101 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     5161 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     5221 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     5281 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     5341 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     5401 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     5461 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     5521 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5581 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5641 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5701 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5761 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5821 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5881 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5941 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6001 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6061 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6121 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6181 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6241 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6301 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6361 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6421 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6481 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6541 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6601 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6661 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6721 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6841 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6901 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6961 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7021 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7081 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7141 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7321 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7381 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7501 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7561 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7621 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7681 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7741 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
     8101 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     8161 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     8221 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     8281 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     8341 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     8401 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     8461 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     8521 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
     8581 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     8641 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
     8701 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
     8761 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
     8821 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     8881 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     8941 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     9001 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     9061 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
     9121 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     9181 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     9241 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     9301 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     9361 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     9421 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     9481 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     9541 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     9601 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     9661 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     9721 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     9781 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     9841 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9961 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaat
//
