LOCUS       sequence-202107-       10245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A 
            mutation in human) in retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula 
            L, Hehnly H
  TITLE     Gravin regulates centrosome function through PLK1.
  JOURNAL   Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524.
  PUBMED    29282278
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524."
COMMENT     Sequence Label: pBABE-SSeCKs T751A
FEATURES             Location/Qualifiers
     source          1..10245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5089..5109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5094..5423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5274..5409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5433..6032
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5433..5452)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5929..5949
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6131..6724
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6925..6942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7096..7684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7176..7195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7862..8653,8654..8722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8485..8504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8723..8827)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8858..9327
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9392..9591
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9792..10208
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10138..10160
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10176..10192
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 caatgggtgc aggcagttcc accgagcagc ggagccccga gcagccggcg gagagcgaca
       61 cgccgagcga gctggagctc agtggccatg ggcccgcagc ggaagcgtcg ggagcagctg
      121 gagatcccgc tgacgcggac cccgccacca agctcccaca gaagaatggt cagctgtctg
      181 ccgtcaatgg tgtagctgaa caagaagatg tccacgtcca agaggaaagc caggatgggc
      241 aagaggaaga agtcactgtt gaagatgttg gacagagaga gtcagaagat gtgaaagaaa
      301 aagaccgagc taaagaaatg gcagccagtt ccacagttgt tgaagatatc acaaaggacg
      361 agcaggagga aacaccggaa ataatcgaac agatccctgc ttcagagagc aatgtggaag
      421 aaatggcgca ggctgctgag tcccaagcta atgacgtcgg cttcaagaag gtatttaaat
      481 ttgttggttt taaattcacg gtgaagaagg ataaaaacga aaagtcagat accgtccagc
      541 tactcactgt caagaaggat gaaggcgaag gggcagaagc ctccgtcgga gcaggagacc
      601 accaagagcc cggagtggag accgtcggcg aatcagcatc caaagaaagt gagctgaagc
      661 aatccacaga gaagcaagaa ggcaccctga agcaagcaca gagcagcaca gaaattcccc
      721 ttcaagccga atctggtcaa gggaccgagg aagaagcagc caaagatgga gaagaaaacc
      781 gagagaaaga acctaccaag cccctagaat ctccgaccag ccctgtcagc aatgagacaa
      841 catcttcctt caagaaattc ttcactcacg gctgggccgg ctggcgcaag aagaccagct
      901 tcaagaaacc aaaggaagat gatctggaaa cttccgagaa gagaaaggag caagaggctg
      961 aaaaagtaga cgaggaagaa ggggaaaaga cagagccagc cccagccgag gagcaggagc
     1021 ctgcagaagg cacagaccag gccaggttgt cagccgacta tgagaaggtg gagttgcctt
     1081 tggaagacca ggtcggtgac ctggaggcat tgtcggagaa gtgtgctcct ttggcaacgg
     1141 aagtgtttga tgagaagacg gaagcccacc aagaagttgt tgcagaggtc cacgtgagca
     1201 ccgtggagaa gatgacgaaa gggcaaggag gagcagaggt ggaaggggat gtggtggtgg
     1261 aaggatcggg agaatccttg ccccctgaga aactggctga gacccaggag gtcccccagg
     1321 aagctgagcc tgtggaggag ctgatgaaga ccaaagaagt atgcgtctct gggggtgacc
     1381 atactcagct gacagatcta agtcctgaag agaagatgct acccaaacac cccgaaggca
     1441 ttgtcagtga ggtggagatg ctgtcctctc aggagagaat caaggtacag ggaagtcccc
     1501 tgaagaagct cttcagcagt tcgggcttaa agaagctctc cgggaagaag cagaagggga
     1561 agagaggagg aggcggggga gatgaagagc caggagaata ccaacacatt caaaccgagt
     1621 ccccagagag tgctgacgag cagaagggag agagctctgc ctcttcccct gaagagcccg
     1681 aggagatcgc gtgtctggag aaggggccat cggaagcacc ccaggaagcg gaagctgagg
     1741 aaggagcgac ttccgacgga gagaagaaaa gggaagggat caccccctgg gcatccttca
     1801 aaaagatggt gacacccaag aaacgggtcc gaagaccttc tgagagcgac aaggaagaag
     1861 agctggataa ggtcaagagt gccaccttgt cctccacgga gagcacggcg tctggaatgc
     1921 aggatgaggt cagagcggtt ggcgaggagc aaaggtcaga ggagccaaag cgcagggtgg
     1981 atacttcagt gtcttgggag gcgttgattt gtgtcggatc gtccaagaag agagcgagga
     2041 aggcatcctc ttcagatgat gaaggagggc caagaacact gggaggggat ggccacagag
     2101 cggaggaggc tagcaaagac aaagaagcag atgctcttcc tgccagcacc caggaacaag
     2161 accaagcgca cggaagttcc tcacccgagc cagctggaag cccttctgaa ggggagggcg
     2221 tctccacctg ggagtcattt aagagattag tcgcaccacg aaaaaaatcc aagtcaaaac
     2281 tggaagagag agccgaagac tccggtgcag agcagttggc ctccgagatc gaaccaagta
     2341 gagaggaatc ttgggtttcc attaagaaat ttattcctgg acggcggaag aaaagggcag
     2401 atgggaagca agaacaggcc gccgttgaag actcggggcc aggagagatc aatgaggacg
     2461 accccgacgt cccagctgtt gtgcctctgt ctgagtacga tgcggtagag agagagaagc
     2521 tggaagcgca gcgagctcag gagaacgtgg agctgcccca gctgaagggg gctgtgtatg
     2581 tgtctgagga gcttagtaag actctggttc acaccgtgag tgtcgcggtc attgatggga
     2641 ccagggcagt caccagtgcc gaagagcggt ccccttcgtg gatatctgct tccatgacag
     2701 aacctcttga gcacgcagag ggagtggcca caccgcctgt tggagaggtc actgaaaaag
     2761 acatcactgc agaagcaact cctgcactcg cccagacttt accagggggc aaagatgccc
     2821 atgacgacat agtcaccagt gaagtggatt ttacctcaga agcagtgaca gccgcagaaa
     2881 ccacagaggc gctccgcgct gaagaactta ccgaagcatc aggggcagaa gagaccacag
     2941 acatggtgtc tgcagtttcc cagctgtccg actccccgga caccacagag gaagccaccc
     3001 cagttcagga ggtagagggt ggcatgctag atacggaaga acaggagcgc cagacgcagg
     3061 ccgtcctcca agccgttgca gacaaagtga aagaggactc ccaggtgcct gcaacccaga
     3121 ctctgcagag agcagggccg aaagcactgg agaaggtgga ggaggtagag gaggactccg
     3181 aggtgctggc taccgagaaa gagaaggatg ttgtgccgga aggacccgtg caggaagctg
     3241 aaactgagca tcttgcacag ggctccgaga ctgtacaggc taccccagag agccttgaag
     3301 ttcctgaagt cacagaggat gtagaccgtg ccaccacatg ccaggttatc aagcaccagc
     3361 agctgatgga acaggctgtg gcccctgagt catctgaaac cttgacagac agtgagacaa
     3421 atggaagtac tcccctcgca gattcagaca ctccaaacgg gacacagcaa gacgagaccg
     3481 ttgacagcca ggacagtaat gccattgccg ccgtcaagca gtcacaggtc actgaagagg
     3541 aggcagctgc tgctcagacg gaggggcctt caacaccatc tagttttcca gcccaggaag
     3601 aacacaggga aaaaccagga agggatgttc tagaacccac acaagcgctg gctgccgggg
     3661 cagtgcctat tctggcaaag gctgaggtgg gtcaagaggg tgaggctggc cagtttgatg
     3721 gagaaaaagt caaagacgga cagtgtgtta aagaactgga ggtgcctgtg cacactggac
     3781 ccaacagtca aaagactgct gacttgacac gtgacagtga agtaatggaa gtggccagat
     3841 gtcaggaaac tgagagtaat gaagaacaga gtattagccc ggagaaaaga gagatgggaa
     3901 ccgacgttga aaaggaggaa acagagacca agacagagca agccagtgaa gaacatgagc
     3961 aggaaacagc tgctcctgag catgaaggaa cccaccctaa gccagtcctg acagctgaca
     4021 tgcctcactc agagagggga aaggcactgg gcagccttga aggaagccct tctctcccag
     4081 accaagacaa agcagattgc atagaggttc aagttcaaag ctcagacaca ccagtcactc
     4141 aaacaaccga agctgtgaaa aaggtcgaag aaactgtggc aacttcagag atggatgaaa
     4201 gtttggagtg tgcaggtgcg caatcattac cagctgagaa gctctccgaa accggtggct
     4261 acgggactct tcagcatgga gaggacaccg tgccccaggg gcctgagtct caggcagagt
     4321 ccatcccaat aatagtaact cctgctcctg aaagcatcct acattctgac cttcaaagag
     4381 aagtgagcgc atcccagaaa cagagatcag atgaagataa caagccagat gctggtcctg
     4441 atgctgccgg caaggagagt gcagcaagag agaaaatcct cagggctgaa cctgagatct
     4501 tggaacttga gagtaagagc aataagattg tccagagtgt catccagaca gccgtcgacc
     4561 agtttgcacg tacagaaaca gcccccgaaa cccacgcttc tgatttacag aatcaggttc
     4621 ctgtgatgca ggctgacagc cagggagcac aacagatgct ggacaaagat gaaagcgacc
     4681 ttcaagtctc cccccaagat ggaacactca gtgccgtagc ccaggaagga cttgcggttt
     4741 ctgatagttc tgaaggcatg agcaaggctt cagaaatgat caccacgctt gcagttgaaa
     4801 gtgccagtgt caaagaaagt gttgagaagc tgcctcttca gtgcaaagat gaaaaggaac
     4861 atgctgctga cggcccccag caccaaagct tagccaaggc agaggcggat gcctctggaa
     4921 atctaaccaa agagtcccca gacaccaacg gaccaaagct aaccgaggaa ggagatgccc
     4981 tgaaagaaga aatgaacaag gcccagacag aagaggacga cctacaggag ccaaagggag
     5041 acctgacaga atcctaactc gagtcgccag cacagtggtc gaccctgtgg aatgtgtgtc
     5101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5161 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5221 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5281 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5341 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5401 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
     5461 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5521 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5581 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5641 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5701 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5761 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5821 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5881 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5941 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga
     6001 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     6061 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga
     6121 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     6181 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     6241 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     6301 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     6361 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     6421 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6481 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6541 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     6601 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     6661 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     6721 catttccgac ttgtggtctc gctgccttgg gagggtctcc tctgagtgat tgactacccg
     6781 tcagcggggg tcttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     6841 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6901 tgcgtattgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7801 aaaatgaagt ttgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7861 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7921 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7981 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8041 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8101 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8161 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8221 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8281 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8341 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8401 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8461 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8521 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8581 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8641 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8701 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8761 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8821 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
     8881 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     8941 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     9001 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     9061 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     9121 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     9181 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     9241 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     9301 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
     9361 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
     9421 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
     9481 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
     9541 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
     9601 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     9661 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     9721 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     9781 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     9841 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     9901 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     9961 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
    10021 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
    10081 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
    10141 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
    10201 taggcgccgg ccggatccca gtgtggtggt acgtaggaat gaatt
//
